Molecular phylogeny and morphology reveal a new epiphytic species of Habenaria (Orchidaceae; Orchideae; Orchidinae) from Nepal
Autoři:
Bhakta Bahadur Raskoti aff001; Rita Ale aff001
Působiště autorů:
Nature Research and Conservation Initiatives, Pokharathok, Arghakhanchi, Nepal
aff001; Nepal Bioscience Research Laboratory, Banasthali, Kathmandu, Nepal
aff002
Vyšlo v časopise:
PLoS ONE 14(10)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0223355
Souhrn
Habenaria is almost cosmopolitan in distribution having predominantly terrestrial orchids, however; a remarkable epiphytic species with some unique morphological characters was collected from Nepal. We conducted a molecular phylogeny of this unusual Habenaria species using nuclear (ITS) and chloroplast (matK, rbcl) DNA sequence regions to infer its systematic position. Our molecular analyses and morphological treatment recognized this newly collected plant as an undescribed species. This species is described here which is closely related to Habenaria plurifoliata but can be distinguished by having its multiple growing callus-shaped tuber, smaller stature with short stem, longer and wider oblanceolate leaves, peduncle with a foliaceous bract and only one sterile bract, lateral sepals oblong, obtuse, petal apex obtuse, lateral lobes of lip spreading upwards, spur not exceeding the ovary and pedicel.
Klíčová slova:
Phylogenetics – Phylogenetic analysis – Chloroplasts – Sepals – Petals – New species reports – Tubers – Plant ovary
Zdroje
1. Govaerts R, Bernet P, Kratochvil K, Gerlach G, Carr G, Alrich P, et al. World Checklist of Orchidaceae. Richmond, U. K.: The Board of Trustees of the Royal Botanic Gardens, Kew. 2019. http://apps.kew.org/wcsp/ (accessed: 2 July 2019).
2. Pridgeon AM, Cribb PJ, Chase MW. Genera Orchidacearum: Vol 2. Orchidoideae: Oxford University Press; 2001.
3. King G, Pantling R. The Orchids of the Sikkim-Himalaya Vol VIII: Dehra Dun: Bishen Singh Mahendra Pal Singh; 1898.
4. Seidenfaden G. Orchid genera in Thailand, Vol. 5, Orchidoideae. Copenhagen: Dansk Botanisk Forening. 1977.
5. Chen X, Cribb PJ. Habenaria. In: Wu ZG, Raven PH & Hong DY. Editors. Flora of China. Vol. 25. Science Press Beijing and Missouri Botanical Garden (St.Louis); 2009. pp. 144–160.
6. Jin WT, Jin XH, Schuiteman A, Li DZ, Xiang XG, Huang WC, et al. Molecular systematics of subtribe Orchidinae and Asian taxa of Habenariinae (Orchideae, Orchidaceae) based on plastid matK, rbcL and nuclear ITS. Mol. Phylogenet. Evol. 2014; 77:41–53. https://doi.org/10.1016/j.ympev.2014.04.004 24747003
7. Jin WT, Schuiteman A, Chase MW, Li JW, Chung SW, Hsu TC, et al. Phylogenetics of subtribe Orchidinae s.l. (Orchidaceae; Orchidoideae) based on seven markers (plastid matK, psaB, rbcL, trnL-F, trnH-psba, and nuclear nrITS, Xdh): implications for generic delimitation. BMC Plant Biol. 2017; 17(1):222. https://doi.org/10.1186/s12870-017-1160-x 29178835
8. Batista JA, Borges KS, de Faria MW, Proite K, Ramalho AJ, Salazar GA, et al. Molecular phylogenetics of the species-rich genus Habenaria (Orchidaceae) in the New World based on nuclear and plastid DNA sequences. Mol. Phylogenet. Evol. 2013; 67(1):95–109. https://doi.org/10.1016/j.ympev.2013.01.008 23337176
9. Pedron M, Buzatto CR, Ramalho AJ, Carvalho BM, Radins JA, Singer RB, et al. Molecular phylogenetics and taxonomic revision of Habenaria section Pentadactylae (Orchidaceae, Orchidinae). Bot. J. Linn. Soc. 2014; 175(1):47–73. https://doi.org/10.1111/boj.12161
10. Long B, Hu GU, Kamande EM, Mutele NV, Wei N. Habenaria luquanensis (Orchidaceae), a new species from Yunnan, China. Phytotaxa. 2015; 238(2):190–195. http://dx.doi.org/10.11646/phytotaxa.238.2.7
11. Kolanowska M. Habenaria fimbriatiloba (Orchidaceae), a new orchid species from China. Phytotaxa. 2015; 201(1):96–99. http://dx.doi.org/10.11646/phytotaxa.201.1.8
12. Kolanowska M, Szlachetko DL, Kras M. Habenaria pseudorostellifera, a new orchid species from China. Ann. Bot. Fennici 2015; 52(5–6), pp. 365–369. https://doi.org/10.5735/085.052.0516
13. Batista JA, Vale AA, Carvalho BM, Proite K, Ramalho AJ, Munhoz AC, et al. Four new species in Habenaria (Orchidaceae) from the Espinhaço Range, Brazil. Syst. Bot. 2016; 41(2):275–92. https://doi.org/10.1600/036364416X691858
14. Lin TP, Huang DM. Newly discovered native orchids of Taiwan (X). Taiwania. 2017; 62(4):349–355. doi: 10.6165/tai.2017.62.349
15. Batista JA, Proite K, de Bem Bianchetti L. Descriptions and phylogenetic relationships of four new species and a new name of Habenaria (Orchidaceae) from the cerrado and campos rupestres of Brazil. Plant Syst. Evol. 2017; 303(7):873–899. https://doi.org/10.1007/s00606-017-1415-x
16. Mejia-Marin MI, Espejo-Serna A, Lopez-Ferral AR Jimenez-Machorro R. Habenaria yookuaaensis (Orchidaceae: Orchidioideae), a new species from Oaxaca, Mexico. Phytotaxa. 2017; 292(1):74–78. http://dx.doi.org/10.11646/phytotaxa.292.1.7
17. Batista JA, Reis AF, JUNIOR JL, Bianchetti LD. Phylogeny and taxonomy of Habenaria leprieurii, H. alpestris and H. sect. Microdactylae (Orchidaceae), with descriptions of two new taxa. Phytotaxa. 2018; 373(4):241–71. http://dx.doi.org/10.11646/phytotaxa.373.4.1
18. Peinado-Arellanes JM. Habenaria ocadiziana (Habenariinae: Orchidaceae) a new and unusual species from western Mexico. Phytotaxa. 2018; 334(3):241–247. http://dx.doi.org/10.11646/phytotaxa.334.3.5
19. Zhang W, Gao JY, Pan B, Liu Q. Habenaria malipoensis (Orchidaceae: Orchidoideae: Orchidinae), a new orchid species from Yunnan, China. Phytotaxa. 2017; 332(1):93–97. https://doi.org/10.11646/phytotaxa.332.1.11
20. Raskoti BB, Ale R. New species of orchids and notes on Orchidaceae of Nepal. Phytotaxa. 2019; 394(4):257–266. https://doi.org/10.11646/phytotaxa.394.4.3
21. Doyle JJ, Doyle JL. A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem. Bull. 1987; 19:11–15.
22. Raskoti BB, Jin WT, Xiang XG, Schuiteman A, Li DZ, Li JW, et al. A phylogenetic analysis of molecular and morphological characters of Herminium (Orchidaceae, Orchideae): evolutionary relationships, taxonomy, and patterns of character evolution. Cladistics. 2016; 32(2):198–210. https://doi.org/10.1111/cla.12125
23. Pridgeon AM, Bateman RM, Cox AV, Hapeman JR, Chase MW. Phylogenetics of subtribe Orchidinae (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Intergeneric relationships and polyphyly of Orchis sensu lato. Lindleyana. 1997; 12:89–109.
24. Bateman RM, Hollingsworth PM, Preston J, Yi-Bo L, Pridgeon AM, Chase MW. Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidaceae). Bot. J. Linn. Soc. 2003; 142(1):1–40. https://doi.org/10.1046/j.1095-8339.2003.00157.x
25. Inda LA, Pimentel M, Chase MW. Phylogenetics of tribe Orchideae (Orchidaceae: Orchidoideae) based on combined DNA matrices: inferences regarding timing of diversification and evolution of pollination syndromes. Ann. Bot. 2012; 110(1):71–90. https://doi.org/10.1093/aob/mcs083 22539542
26. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997; 25(24):4876–4882. https://doi.org/10.1093/nar/25.24.4876 9396791
27. Rambaut A. Se-Al sequence alignment editor, version 2.0 a11. University of Oxford. Oxford, UK; 2002.
28. Farris JS, Källersjö M, Kluge AG, Bult C. Testing significance of incongruence. Cladistics. 1994; 10(3):315–319. https://doi.org/10.1111/j.1096-0031.1994.tb00181.x
29. Swofford DL. Phylogenetic analysis using parsimony (* and other methods). Version 4.0b10 [computer program]. Sunderland, Massachusetts (USA): Sinauer Associates; 2002.
30. Darlu P, Lecointre G. When does the incongruence length difference test fail? Mol. Biol. Evol. 2002; 19(4):432–437. https://doi.org/10.1093/oxfordjournals.molbev.a004098 11919284
31. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985; 39(4):783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x 28561359
32. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, et al. MrBayes 3.2: efficient Bayesian Phylogenetic inference and model choice across a large model space. Syst. Biol. 2012; 61(3):539–42. https://doi.org/10.1093/sysbio/sys029 22357727
33. Posada D, Crandall KA. Modeltest: testing the model of DNA substitution. Bioinformatics. 1998; 14(9):817–818. https://doi.org/10.1093/bioinformatics/14.9.817 9918953
34. Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshop (GCE), New Orleans; 2010. pp. 1–8.
35. IUCN Standard and Petitions Subcommittee. Guidelines for Using the IUCN Red List Categories and Criteria, version 13. Prepared by the Standards and Petitions Subcommittee. 2017. Downloadable from http://www.iucnredlist.org/documents/RedListGuidelines.pdf.
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