Detection and determination of stability of the antibiotic residues in cow’s milk
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Mahantesh Kurjogi aff001; Yasser Hussein Issa Mohammad aff002; Saad Alghamdi aff003; Mostafa Abdelrahman aff004; Praveen Satapute aff001; Sudisha Jogaiah aff001
Působiště autorů:
Laboratory of Plant Healthcare and Diagnostics, Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India
aff001; Department of Biochemistry, Applied Science college, Hajjah University, Hajjah, Yemen
aff002; Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia
aff003; Arid Land Research Center, Tottori University, Tottori, Japan
aff004
Vyšlo v časopise:
PLoS ONE 14(10)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0223475
Souhrn
In the present study, antibiotic residues were detected in milk samples collected from the dairy herds located in Karnataka, India, by microbiological assay. Subsequently, the detected antibiotics were identified as azithromycin and tetracycline, by high-performance liquid chromatography, further both the antibiotics detected in the cow milk samples were found to be at high concentration (9708.7 and 5460 μg kg-1, respectively). We then investigated the effects of temperature and pH on the stabilities of azithromycin and tetracycline to determine the degradation rate constant k using first-order kinetic equation. Results indicated that significant reduction in stability and antibacterial activity of azithromycin solution when subjected to 70 and 100°C for 24 h. While stability of tetracycline was significantly reduced when subjected to 70 and 100°C for 24 h. However no significant reduction in antibacterial activity of tetracycline was observed at respective temperatures when compared with that of control. In addition, the stabilities of azithromycin and tetracycline were found to be decreased in acidic pH 4–5. The results of the present study revealed the high risk of contamination of milk sample with veterinary antibiotics and also demonstrated the effect of temperature and pH on stability of antibiotics. Therefore the study suggest that the qualitative and quantitative screening of milk for the presence of antibiotics need to be strictly performed to ensure safe drinking milk for consumers.
Klíčová slova:
Antimicrobials – Antibiotics – Milk – High performance liquid chromatography – Antibacterials – Tetracyclines – Bacillus subtilis – Penicillin
Zdroje
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