Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue

English version

Autoři: Marie-Ève Ouellette ^aff001; Jean-Christophe Bérubé ^aff003; Jean-Michel Bourget ^aff001; Maud Vallée ^aff001; Yohan Bossé ^aff003; Julie Fradette ^aff001
Působiště autorů: Centre de Recherche en Organogenèse Expérimentale de l'Université Laval/LOEX, Division of Regenerative Medicine, CHU de Québec -Université Laval Research Center, Québec, QC, Canada ^aff001; Department of Surgery, Faculty of Medicine, Université Laval, Québec, Canada ^aff002; Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada ^aff003; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Québec, Canada ^aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0224228

Souhrn

Reconstructed human adipose tissues represent novel tools available to perform in vitro pharmaco-toxicological studies. We used adipose-derived human stromal/stem cells to reconstruct, using tissue engineering techniques, such an adipose tridimensional model. To determine to what extent the in vitro model is representative of its native counterpart, adipogenic differentiation, triglycerides accumulation and phospholipids profiles were analysed. Ingenuity Pathway Analysis software revealed pathways enriched with differentially-expressed genes between native and reconstructed human adipose tissues. Interestingly, genes related to fatty acid metabolism were downregulated in vitro, which could be explained in part by the insufficient amount of essential fatty acids provided by the fetal calf serum used for the culture. Indeed, the lipid profile of the reconstructed human adipose tissues indicated a particular lack of linoleic acid, which could interfere with physiological cell processes such as membrane trafficking, signaling and inflammatory responses. Supplementation in the culture medium was able to influence the lipid profile of the reconstructed human adipose tissues. This study demonstrates the possibility to directly modulate the phospholipid profile of reconstructed human adipose tissues. This reinforces its use as a relevant physiological or pathological model for further pharmacological and metabolic studies of human adipose tissue functions.

Klíčová slova:

Lipids – Phospholipids – Fatty acids – Fats – Adipose tissue – Lipid analysis – Lipid metabolism – Adipocytes

Zdroje

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Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue

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