Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop
Autoři:
Philippe Champeil aff001; Béatrice de Foresta aff001; Martin Picard aff001; Carole Gauron aff001; Dominique Georgin aff002; Marc le Maire aff001; Jesper V. Møller aff003; Guillaume Lenoir aff001; Cédric Montigny aff001
Působiště autorů:
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France
aff001; CEA, DSV, Institut des Sciences du Vivant Frédéric Joliot, Service de Chimie Biorganique et de Marquage, Gif-sur-Yvette, France
aff002; Center for Membrane Pumps in Cells and Disease, PUMPKIN, Danish National Research Foundation, Aarhus University, Aarhus, Denmark
aff003; Department of Biomedicine, Aarhus University, Aarhus, Denmark
aff004
Vyšlo v časopise:
PLoS ONE 14(10)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222932
Souhrn
The present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of C12E8 and now stopped-flow fluorescence instead of rapid filtration, we found that the rate of dissociation of this detergent from these membranes, merely perturbed with non-solubilizing concentrations of detergent, was significantly faster (t1/2 < 10 ms) than what had previously been determined (t1/2 ~300–400 ms) from experiments based on a rapid filtration protocol using 14C-labeled C12E8 and glass fiber filters (Binding of a non-ionic detergent to membranes: flip-flop rate and location on the bilayer, by Marc le Maire, Jesper Møller and Philippe Champeil, Biochemistry (1987) Vol 26, pages 4803–4810). We here pinpoint a methodological problem of the earlier rapid filtration experiments, and we suggest that the true overall dissociation rate of C12E8 is indeed much faster than previously thought. We also exemplify the case of brominated dodecyl-maltoside, whose kinetics for overall binding to and dissociation from membranes comprise both a rapid and a sower phase, the latter being presumably due to flip-flop between the two leaflets of the membrane. Consequently, equilibrium is reached only after a few seconds for DDM. This work thereby emphasizes the interest of using the fluorescence quenching associated with brominated detergents for studying the kinetics of detergent/membrane interactions, namely association, dissociation and flip-flop rates.
Klíčová slova:
Lipids – Vesicles – Equipment – Detergents – Membrane electrophysiology – Chemical dissociation – Filtration – Light scattering
Zdroje
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