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Successful development of methodology for detection of hapten-specific contact hypersensitivity (CHS) memory in swine


Autoři: E. J. Putz aff001;  A. M. Putz aff001;  A. Boettcher aff001;  S. Charley aff001;  M. Sauer aff001;  M. Palmer aff003;  R. Phillips aff002;  J. Hostetter aff002;  C. L. Loving aff004;  J. E. Cunnick aff001;  C. K. Tuggle aff001
Působiště autorů: Iowa State University, Department of Animal Science, Ames, IA, United States of America aff001;  Iowa State University College of Veterinary Medicine, Department of Veterinary Pathology Science, Ames, IA, United States of America aff002;  USDA-ARS-National Animal Disease Center, Infectious Bacterial Diseases of Livestock Research Unit, Ames, IA, United States of America aff003;  USDA-ARS-National Animal Disease Center, Food Safety and Enteric Pathogens Research Unit, Ames, IA, United States of America aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0223483

Souhrn

Hapten contact hypersensitivity (CHS) elicits a well-documented inflammation response that can be used to illustrate training of immune cells through hapten-specific CHS memory. The education of hapten-specific memory T cells has been well-established, recent research in mice has expanded the “adaptive” characteristic of a memory response from solely a function of the adaptive immune system, to innate cells as well. To test whether similar responses are seen in a non-rodent model, we used hapten-specific CHS to measure the ear inflammation response of outbred pigs to dinitrofluorobenzene (DNFB), oxazolone (OXA), or vehicle controls. We adapted mouse innate memory literature protocols to the domestic pig model. Animals were challenged up to 32 days post initial sensitization exposure to the hapten, and specific ear swelling responses to this challenge were significant for 7, 21, and 32 days post-sensitization. We established hapten-specific CHS memory exists in a non-rodent model. We also developed a successful protocol for demonstrating these CHS responses in a porcine system.

Klíčová slova:

Inflammation – Necrosis – Ears – Swine – Pig models – Haptens – Intradermal injections – NK cells


Zdroje

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