Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis

English version

Autoři: Maximilianos Kotsias ^aff001; Athanasios Blanas ^aff002; Sandra J. van Vliet ^aff002; Martina Pirro ^aff003; Daniel I. R. Spencer ^aff001; Radoslaw P. Kozak ^aff001
Působiště autorů: Ludger Ltd., Culham Science Centre, Abingdon, Oxfordshire, England, United Kingdom ^aff001; Amsterdam UMC, Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam, The Netherlands ^aff002; Leiden University Medical Centre, Centre for Proteomics and Metabolomics, Leiden, The Netherlands ^aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0223270

Souhrn

The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.

Klíčová slova:

Blood plasma – Colorectal cancer – Glycosylation – Consortia – Galactose – HT29 cells – Hexoses – Mannose

Zdroje

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Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis

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Zdroje

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