Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads

English version

Autoři: Seung Joon Lee ^aff001; Tae Seok Sim ^aff003; Hyun Young Shin ^aff001; Jungmin Lee ^aff001; Min Young Kim ^aff001; Joseph Sunoo ^aff002; Jeong-Gun Lee ^aff003; Kyungmoo Yea ^aff001; Young Zoon Kim ^aff004; Danny van Noort ^aff001; Soo Kyung Park ^aff002; Woon-Hae Kim ^aff001; Kyun Woo Park ^aff006; Minseok S. Kim ^aff001
Působiště autorů: Department of New Biology, DGIST, Daegu, Republic of Korea ^aff001; CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea ^aff002; Samsung Electronics, Ltd., Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea ^aff003; Division of Neurooncology and Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Republic of Korea ^aff004; Division of Biotechnology, IFM, Linköping University, Linköping, Sweden ^aff005; Daejeon Wellness Hospital, Beon-gil, Dongseo-daero, Daedeok-gu, Daejeon, Republic of Korea ^aff006; Translational Responsive Medicine Center, DGIST, Daegu, Republic of Korea ^aff007
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0223193

Souhrn

Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs.

Klíčová slova:

Blood – Blood cells – Glass – Breast cancer – Fluid flow – Flow rate – Microfluidics – Microbeads

Zdroje

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Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads

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