Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
Autoři:
Seung Joon Lee aff001; Tae Seok Sim aff003; Hyun Young Shin aff001; Jungmin Lee aff001; Min Young Kim aff001; Joseph Sunoo aff002; Jeong-Gun Lee aff003; Kyungmoo Yea aff001; Young Zoon Kim aff004; Danny van Noort aff001; Soo Kyung Park aff002; Woon-Hae Kim aff001; Kyun Woo Park aff006; Minseok S. Kim aff001
Působiště autorů:
Department of New Biology, DGIST, Daegu, Republic of Korea
aff001; CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
aff002; Samsung Electronics, Ltd., Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea
aff003; Division of Neurooncology and Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Republic of Korea
aff004; Division of Biotechnology, IFM, Linköping University, Linköping, Sweden
aff005; Daejeon Wellness Hospital, Beon-gil, Dongseo-daero, Daedeok-gu, Daejeon, Republic of Korea
aff006; Translational Responsive Medicine Center, DGIST, Daegu, Republic of Korea
aff007
Vyšlo v časopise:
PLoS ONE 14(10)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0223193
Souhrn
Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs.
Klíčová slova:
Blood – Blood cells – Glass – Breast cancer – Fluid flow – Flow rate – Microfluidics – Microbeads
Zdroje
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