Expression of Concern: The Coordination of Cell Growth during Fission Yeast Mating Requires Ras1-GTP Hydrolysis
Authors:
Published in the journal:
PLoS ONE 14(10)
Category:
Expression of Concern
doi:
https://doi.org/10.1371/journal.pone.0223817
After publication of this article [1], the following concerns were raised:
In Fig 8A, abrupt discontinuities are visible when the image for the strain Ras1G17V at 0 μM is adjusted for brightness.
In Fig 8C, abrupt discontinuities are visible in the following panels when adjusted for brightness/contrast. The discontinuities suggest that the image in each panel of concern may not represent continuous data from the same photograph.
◦ wild type transformed with vector
◦ wild type transformed with pCdc42
◦ Δgap1 transformed with pCdc42
◦ ras1Q66L transformed with vector
◦ ras1Q66L transformed with pPob1
Similarities were noted between the images in the following panels:
◦ Fig 6C Δgap1Δbyr2/0 μM, Fig 6C Δgap1+pScd1/0 μM, and Fig 7C Ras1G17V/0 μM
◦ Fig 6C Δgap1/0 μM and Δgap1Δbyr2/10 μM
◦ Fig 6A Δbyr2/0 μM (right) and Fig 7C Ras1Q66L/0 μM
The authors claimed that Fig 8A and 8C panels each show a composite montage of cell images captured from nonadjacent areas of the same slide, similar to Figure 9. They provided an updated version of Fig 8C in which image splice lines are indicated.
They also noted that errors were made in preparing figures which led to the duplications in Figs 6 and 7. In the published figures, the incorrect images are shown in Fig 6C for Δgap1/0 μM and Δgap1+pScd1/0 μM; and in Fig 7C for Ras1G17V/0 μM and Ras1Q66L/0 μM. The authors provided revised figures in which the errant panels in Figs 6 and 7 have been replaced with the correct data from the original experiments.
Please see the updated Figs 6–8 and their accompanying captions here. Note, legends for Figs 6 and 7 are unchanged from the published article.
The underlying raw image data supporting the figures discussed above are no longer available.
Due to the nature and extent of image issues and lack of underlying image data for the figures in question, the PLOS ONE Editors issue this Expression of Concern.
Zdroje
1. Weston C, Bond M, Croft W, Ladds G (2013) The Coordination of Cell Growth during Fission Yeast Mating Requires Ras1-GTP Hydrolysis. PLoS ONE 8(10): e77487. https://doi.org/10.1371/journal.pone.0077487 24147005
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