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preLekára.sk / Odborné časopisy / PLOS One / 2019 - 10

Optimized bioluminescence analysis of adenosine triphosphate (ATP) released by platelets and its application in the high throughput screening of platelet inhibitors

Authors: Lili Wang aff001;  Yunqian Li aff001;  Ran Guo aff001;  Shanshan Li aff001;  Anqi Chang aff001;  Zhixiang Zhu aff001;  Pengfei Tu aff001
Authors place of work: Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China aff001
Published in the journal: PLoS ONE 14(10)
Category: Research Article
doi: https://doi.org/10.1371/journal.pone.0223096

Summary

Activated platelets release adenosine trisphosphate (ATP) and bioluminescence analysis of ATP release is usually used to monitor activation of platelets induced by various stimulants. However, bioluminescence analysis of ATP possesses poor linearity, the signal is quickly attenuated, and the accuracy of ATP release from platelets is hard to determine accurately enough to be used in a high throughput screening of platelet inhibitors. The present study was designed to optimize bioluminescence analysis of ATP released by platelets and expand its application in high throughput screening of platelet inhibitors. The results showed that accuracy of ATP analysis was significantly improved by adding coenzyme A (CoA) and signal attenuation of ATP analysis was greatly postponed by adding bovine serum albumin (BSA) both in Hank’s balanced salt solution (HBSS) and Tyrode’s buffer. Furthermore, ATP release of activated platelets and inhibitory effects of Ly294002 and Staurosporine on platelet activation were accurately determined by our optimized bioluminescence analysis of ATP. Thus, we have successfully constructed an optimized bioluminescence analysis of ATP which can be used in high throughput screening of platelet inhibitors.

Keywords:

Enzymes – Collagens – Luciferase – Platelets – Platelet activation – Bioluminescence – Thrombin – Reaction systems

Introduction

Platelets are a component of blood and play important roles in hemostasis. In platelets, there are two different types of releasable granules which are named as alpha granules and dense granules. Alpha granules contain various presynthesized thrombogenic proteins, such as von Willebrand factor (vWF), fibronectin, and P-selectin. Dense granules contain small molecules, especially adenosine diphosphate (ADP), adenosine triphosphate (ATP) and serotonin [1]. After circulating platelets are exposed to collagen-rich subendothelium at the site of vascular injury or thrombin, these granules are secreted, which is essential for the recruitment and activation of further platelets, thereby promoting platelet aggregation, thrombus formation, and wound repair [2, 3]. On the other hand, platelet activation in pathologic situations can lead to thrombosis, causing myocardial infarction or stroke. In order to develop novel inhibitors of platelet activation for the prevention and treatment of thrombotic diseases, it is necessary to analyze platelet activation accurately and conveniently.

As quantitative determination of ATP based on bioluminescence reaction catalyzed by firefly luciferase is a superior method with high sensitivity and specificity, platelet activation can be detected through analyzing ATP release of activated platelets [4]. Therefore, bioluminescence analysis of ATP can also be used to screen platelet inhibitors [5–7]. However, there are still some defects in the quantitative analysis of ATP released by platelets, including poor accuracy and excessive attenuation of bioluminescence.

Luciferase catalyzes the oxidation of D-luciferin (LH2) with molecular oxygen in the presence of ATP and Mg2+ with emission of yellow-green light in a highly efficient process. This reaction involves the formation of luciferyl adenylate intermediate (LH2-AMP) with the release of pyrophosphate. LH2-AMP not only can be oxidized into oxyluciferin accompanied by release of AMP, carbon dioxide, and light emission, but also be oxidized into dehydroluciferyl adenylate (L-AMP) which is a side product (about 20% of the reacted LH2) of the bioluminescence reaction. Undesirably, L-AMP has been identified as a powerful luciferase inhibitor with an IC50 of about 6 nM and significantly impacts bioluminescence reaction rate of ATP analysis catalyzed by luciferase, especially at high concentration of ATP [8–10]. The inhibitory effects of L-AMP on bioluminescence reaction result in unsatisfactory linearity and accuracy of ATP analysis. Furthermore, quick consumption of ATP in the bioluminescence reaction leads to excessive signal attenuation. These defects in the bioluminescence analysis of ATP seriously restricted its application in the high throughput screening of platelet inhibitors.

In the present study, we investigated the effects of coenzyme A (CoA) on linearity improvement and the effects of bovine serum albumin (BSA) on signal attenuation in bioluminescence analysis of ATP. Subsequently, we explored applicability of optimized bioluminescence analysis of ATP in platelet activation assay and high throughput screening of platelet inhibitors.

Materials and methods

Reagents

A stock solution of luciferase (product number: L9420), 1, 4-dithiothreitol (DTT), adenosine 5’-triphosphate disodium salt (ATP), coenzyme A sodium salt (CoA), BSA, ovalbumin (OVA), luminol sodium salt, 4-(imidazol-1-yl)phenol (4-IMP), hydrogen peroxide solution (H2O2), peroxidase from horseradish (HRP) and thrombin were purchased from Sigma-Aldrich (St. Louis, MO, USA). D-luciferin sodium salt was purchased from J&K Scientific (Beijing, China). Collagen was purchased from Hyphen Biomed (Neuville-sur-Oise, France). Hank’s balanced salt solution (HBSS) was obtained from ThermoFisher Scientific (Waltham, MA, USA). Ly294002 (phosphoinositite 3 kinase (PI3K) inhibitor) and Staurosporine (protein kinase C (PKC) inhibitor) were purchased from Abmole Bioscience (Houston, TX, USA). Prostaglandin E1 (PGE1) and other commonly used chemical reagents were purchased from Aladdin Industrial (Shanghai, China).

Animals

Twelve 3-month-old Male Sprague Dawley rats (body weight, 220 ± 10g) were purchased from SPF Biotechnology (Beijing, China). The rats were maintained at a constant temperature and humidity with a 12 h light/dark cycle, and free access to food and water. All protocols conformed to guidelines in Beijing University of Chinese Medicine (Beijing, China) and the study was approved by the Ethical Committee on Animal Research (2018-03-4328) in Beijing University of Chinese Medicine.

Preparation of washed platelets

Blood was withdrawn from abdominal aorta of anesthetized rat into evacuated tube for blood specimen collection containing 3.8% sodium citrate as anticoagulant. Platelet-rich plasma (PRP) and washed platelets were prepared as described previously [1–3]. In brief, immediately after blood collection, rat’s blood was centrifuged at 200 g for 10 minutes at room temperature and the PRP was carefully separated. Platelets were separated from PRP by centrifugation at 1000 g for 10 minutes and washed with CGS buffer (1.7 mM citric acid, 18.3 mM trisodium citrate, 10 mM glucose, 120 mM sodium chloride, PH 6.5) in the presence of PGE1 (2 μM) and resuspended in buffers of subsequent experiments.

Bioluminescence analysis of ATP by adding CoA

All the enzyme reactions were performed in white 96-well plates at ambient temperature (24–27°C). Firstly, a stock solution of ATP (10 mM) was serially diluted into 1 to 0.031 μM with buffer A (25 mM Tricine-HCl, 5 mM MgSO4, 0.1 mM EDTA, pH 7.8) and added into 96-well plate (100 μl). Then enzyme work solution (2 ×) containing DTT (2 mM), luciferin (1 mM) and luciferase (2.5 μg/ml) was also prepared by buffer A, and added into 96-well plate (100 μl) to initiate bioluminescence reaction. Unless otherwise stated, the plates of bioluminescence analysis were shaken for 10 s after all reagents were added. Relative light unit (RLU) was analyzed on EnSpire Multimode Plate Reader (PerkinElmer, Waltham, MA, USA) at different time points. Secondly, when the concentration of ATP in the enzyme reaction mixture was fixed at 1 μM, a series of concentrations of CoA were added in reaction system and the effects of CoA on bioluminescence assay were analyzed. At last, determination of standard curve of ATP was performed again in the presence of optimized concentration of CoA (15 μM).

Bioluminescence analysis of ATP in HBSS and Tyrode’s buffer

ATP was serially diluted into 1 to 0.031 μM with HBSS (138 mM NaCl, 5.33 mM KCl, 0.34 mM Na2HPO4, 0.44 mM KH2PO4, 4.17 mM NaHCO3, 5.56 mM glucose, 0.41 mM MgSO4, 0.49 mM MgCl2 and 1.26 mM CaCl2, pH 7.3) or modified Tyrode’s buffer (137 mM NaCl, 2.9 mM KCl, 0.34 mM Na2HPO4, 12 mM NaHCO3, 5 mM HEPES, 5 mM glucose, 1 mM MgCl2 and 1 mM CaCl2, pH 7.3) and added into 96-well plate (100 μl). In the presence or absence of CoA (30 μM), enzyme work solution (2 ×) containing DTT (2 mM), luciferin (1 mM) and luciferase (2.5 μg/ml) was also prepared with HBSS or Tyrode’s buffer, and added into 96-well plate (100 μl) to initiate bioluminescence reaction, then RLU was analyzed at different time points.

Bioluminescence analysis of ATP by adding BSA in HBSS and Tyrode’s buffer

ATP was diluted into 1 μM with HBSS or Tyrode’s buffer and added into 96-well plate (100 μl). A series of concentrations of BSA were added in reaction system (50 μl). Ultimately, enzyme work solution (4 ×) containing DTT (4 mM), luciferin (2 mM), luciferase (5 μg/ml) and CoA (60 μM) was added into 96-well plate (50 μl) to initiate bioluminescence reaction and RLU was analyzed at different time points. Subsequently, a series of concentrations of ATP were added into 96-well plate (100 μl). In the presence or absence of BSA (0.05%), the enzyme work solution (2 ×) containing DTT (2 mM), luciferin (1 mM), luciferase (2.5 μg/ml) and CoA (30 μM) was added into 96-well plate (100 μl) to initiate bioluminescence reaction and RLU was analyzed at different time points.

Chemiluminescence analysis of HRP by adding BSA and bioluminescence analysis of ATP by adding OVA

In the presence of absence of 0.05% BSA, enhanced chemiluminescence reaction of luminol and H2O2 was initiated by adding HRP with a final concentration of 0.001 U/ml into 200 μl reaction solution (0.1 M Tris-HCl, 1.25 mM luminol, 3 mM H2O2, 0.5 mM 4-IMP, pH 8.6). RLU was analyzed at different time points. In addition, bioluminescence analysis of ATP (1 μM) was performed in 200 μl reaction system (1 mM DTT, 1 mM luciferin, 1.25 μg/ml luciferase and 15 μM CoA) with or without 0.05% OVA at different time points.

Method validation of ATP analysis in the presence of platelets and bioluminescence analysis of ATP released by activated platelets

Washed platelets were suspended in HBSS or Tyrode’s buffer (2 × 107 platelets/ml) and seeded in white 96-well plate (100 μl). In the presence or absence of CoA (15μM), standard curves of ATP analyses in HBSS or Tyrode’s buffer containing platelets were determined again. In addition, in the presence or absence of BSA (0.05%), signal attenuations of ATP analyses in HBSS or Tyrode’s buffer containing platelets were evaluated. Recovery rates of ATP were investigated by adding standard ATP (0.1μM or 1 μM) into HBSS or Tyrode’s buffer containing platelets and performing quantitative analysis of ATP. At last, the platelet suspension was added into 96-well plate (100 μl) or 384-well plate (20 μl). Subsequently, the platelets were stimulated with thrombin (0.1 U/ml) or collagen (20 μg/ml) at 37°C for 20 min. Bioluminescence analysis of ATP released by activated platelets was initiated by adding enzyme work solution containing CoA and BSA into reaction system and RLU was determined at 5 min after the start of the reaction.

Screening of platelet inhibitors with optimized bioluminescence analysis of released ATP

Washed platelets were resuspended in HBSS or Tyrode’s buffer (2 × 107 platelets/ml) and seeded in white 96-well plate (100 μl). The platelets were pretreated with or without 25 μl compounds for 5 min and stimulated with 25 μl thrombin (0.6 U/ml) or collagen (120 μg/ml) at 37°C for 20 min. Bioluminescence analysis of ATP released by activated platelets was initiated by adding enzyme work solution (4 ×, 50 μl) into 96-well plate and RLU was determined at 5 min after the start of the reaction.

Statistical analysis

The results were expressed as mean ± standard deviation (SD) of triplicates from one representative experiment or three independent experiments as described. Z’ factor was calculated with the formula: Z’ factor = 1 - (3 × (SDagonist + SDvehicle) / (Meanagonist—Meanvehicle). For each condition, Z’ factor was acquired from 96 samples in 96-well plate or from 384 samples in 384-well plate. Half were stimulated platelets and half were non-stimulated platelets. Statistical analysis was performed using GraphPad Prism 5. Unpaired student’s t test or one-way ANOVA followed by Dunnett’s test was used to evaluate differences between experimental groups and P value less than 0.05 was considered to be statistically significant.

Results

Effects of CoA on the bioluminescence analysis of ATP in buffer A

In the bioluminescence analysis of ATP, we first performed standard curve assay in buffer A. As shown in Fig 1A, standard curve of ATP analysis exhibited unsatisfactory linearity and R2 of linear regression analysis was only 0.922. Obviously, RLU values were not proportional to the concentrations of ATP when ATP was at high concentrations. Because CoA can block inhibitory effects of enzyme reaction product on luciferase activity, we attempted to improve linearity of ATP analysis by adding CoA in the bioluminescence reaction system. The results (Fig 1B) showed that RLU values of ATP analysis were significantly affected by CoA and CoA in the concentration range of 7.8 to 31.2 μM enhanced RLU values of ATP analysis to the maximum extent. Thus, we carried out standard curve analysis of ATP again by adding 15 μM CoA in the reaction system. As shown in Fig 1C, the standard curve of ATP analysis was greatly improved and R2 was 0.998. However, the signal attenuation of ATP analysis in buffer A was quick even in the presence of CoA and attenuation rate reached more than 60% in 20min and 90% in 80 min (Fig 1D).

Fig. 1.
CoA improved linear range of bioluminescence analysis of ATP in buffer A. Bioluminescence analysis of ATP was performed in buffer A and standard curve was made (A). The effects of CoA on the signal intensity of ATP analysis were evaluated (B). Then standard curve analysis of ATP (C) was made again in the presence of CoA at optimized concentration (15 μM) and signal attenuation of ATP analysis (D) was also estimated by determining relative light unit (RLU) at different time points. Data are representative of three independent experiments. * P < 0.05 vs CoA (15.6 μM).

Bioluminescence analyses of ATP in HBSS and Tyrode’s buffer

Although luciferase can maintain high activity in buffer A, platelets cannot keep normal function to be activated by various stimulants, such as thrombin, collagen and ADP. So we further evaluated bioluminescence analyses of ATP in HBSS and Tyrode’s buffer. Consistent with the measurements in buffer A, standard curve linearity of ATP analyses in HBSS and Tyrode’s buffer was significantly improved by adding CoA in the bioluminescence reaction system (Fig 2A and 2B). However, signal attenuations of ATP analyses in HBSS and Tyrode’s buffer were still fast even though they were slower than that in buffer A. The attenuation rates reached approximately 30% in 20 min and 50% in 80 min (Fig 2C and 2D).

Fig. 2. Bioluminescence analyses of ATP in HBSS and Tyrode’s buffer have optimized linearity in the presence of CoA.
Bioluminescence analyses of ATP in HBSS and Tyrode’s buffer have optimized linearity in the presence of CoA.
Bioluminescence analysis of ATP was performed in HBSS and Tyrode’s buffer with or without CoA (15 μM) and standard curves were made (A and B). The signal attenuations of ATP analyses in the presence of CoA (C and D) were also estimated by determining RLU at different time points. Data are representative of three independent experiments.

Effects of BSA on the bioluminescence analyses of ATP in HBSS and Tyrode’s buffer

In view of quickly signal attenuations of ATP analyses in HBSS and Tyrode’s, we always tried to slow down signal attenuation for more accurate ATP analysis. In some experiments, we found that signal attenuation of ATP analysis was retarded by adding 0.35% BSA in the Tyrode’s buffer. So we investigated the effects of BSA on the signal attenuations of ATP analyses in HBSS and Tyrode’s buffer. The results showed that BSA not only enhanced RLU values (Fig 3A and 3B), but also slowed down signal attenuations of bioluminescence analyses of ATP in HBSS and Tyrode’s buffer (Fig 3C and 3D). When BSA was at the concentrations of 0.0312% to 0.125%, the RLU values of ATP analyses were enhanced to the maximum extent (Fig 3A and 3B). As for signal attenuation, BSA reduced signal attenuations at all concentrations, especially at the concentrations of 1% (Fig 3C and 3D). Thereafter, we determined standard curves of bioluminescence analyses of ATP in the presence or absence of 0.05% BSA at different time points. The results showed that signal attenuations of ATP analyses were improved by adding BSA both in HBSS (Fig 3E and 3G) and Tyrode’s buffer (Fig 3F and 3H).

Fig. 3. BSA slowed down signal attenuations of bioluminescence analyses of ATP in HBSS and Tyrode’s buffer.
BSA slowed down signal attenuations of bioluminescence analyses of ATP in HBSS and Tyrode’s buffer.
Bioluminescence analyses of ATP (1 μM) were performed in HBSS and Tyrode’s buffer in the presence of CoA (15 μM) and serial concentrations of BSA (A and B). The signal attenuations of ATP analyses in the presence of serial concentrations of BSA were also estimated by RLU80min/RLU5min (C and D). Then in the presence or absence of BSA (0.05%), standard curve analyses of ATP in HBSS (E and G) and Tyrode’s buffer (F and H) were performed at different time points. Data are representative of three independent experiments. * P < 0.05 vs BSA (0.0625%).

Effects of BSA on chemiluminescence analysis of HRP and effects of OVA on the bioluminescence analysis of ATP

In order to identify whether the postponed effects of BSA on signal attenuation was specific to bioluminescence analysis of ATP, we followed to evaluate the effects of BSA on chemiluminescence analysis of HRP activity. As shown in Fig 4A and 4B, signal attenuation of HRP analysis hadn’t been slowed down by adding 0.05% BSA in the reaction system and even the signal intensity of chemiluminescence was decreased by BSA. Furthermore, we also investigated the effects of OVA on the signal attenuation of bioluminescence analysis of ATP. Consistent to the effects of BSA, OVA significantly enhanced RLU values and slowed down signal attenuations of ATP analyses in HBSS (Fig 4C and 4D) and Tyrode’s buffer (Fig 4E and 4F).

Fig. 4. BSA didn’t improve signal attenuation of chemiluminescence analysis of HRP and OVA slowed down signal attenuation of bioluminescence analysis of ATP.
BSA didn’t improve signal attenuation of chemiluminescence analysis of HRP and OVA slowed down signal attenuation of bioluminescence analysis of ATP.
In the presence or absence of BSA (0.05%), chemiluminescence analysis of HRP activity (0.001 U/ml) was performed in enhanced chemiluminescence solution at different time points (A) and signal attenuation was evaluated by RLU20min/RLU5min (B). Bioluminescence analyses of ATP (1 μM) were carried out in HBSS and Tyrode’s buffer with or without OVA (0.05%) at different time points (C and E). The signal attenuations of ATP analyses were analyzed by RLU80min/RLU5min (D and F). Data are representative of three independent experiments. * P < 0.05 vs BSA (0.05%) or OVA (0.05%).

Method validation of ATP analysis in the presence of platelets and bioluminescence analysis of ATP released by activated platelets

After bioluminescence analysis of ATP was optimized by adding CoA and BSA in the reaction system, we subsequently validated their optimized effects in the presence of platelets. The results showed that quantitative linearity of ATP analysis was significantly improved through addition of CoA (Fig 5A and 5B) and signal attenuation of bioluminescence was intensively slowed down through addition of BSA (Fig 5C and 5D). In the presence of CoA and BSA, recovery rates of ATP analyses in HBSS and Tyrode’s buffer were also optimized (95% - 105%) compared to control (65% - 90%) (Fig 5E and 5F). Subsequently, the optimized bioluminescence analysis of ATP was used to monitor ATP release of activated platelets. The results (Fig 5G and 5H) showed that ATP releases of activated platelets induced by thrombin and collagen could be well monitored by the optimized bioluminescence analysis of ATP. The ATP releases of platelets in HBSS and Tyrode’s buffer exhibited similar magnitudes. Z’ factors of ATP releases induced by thrombin and collagen in HBSS and Tyrode’s were all over 0.5. Similar results were acquired in experiments of 96-well plate and 384-well plate.

Fig. 5. Method validation of ATP analysis in the presence of platelets and bioluminescence analysis of ATP released by activated platelets.
Method validation of ATP analysis in the presence of platelets and bioluminescence analysis of ATP released by activated platelets.
Washed platelets were suspended in HBSS or Tyrode’s buffer and seeded in white 96-well plate. In the presence of platelets, standard curves of ATP were performed by adding of CoA (15 μM) into reaction system (A and B) and signal attenuation curves were analyzed by adding of BSA (0.05%) into reaction system (C and D). Data are representative of three independent experiments. Recovery rates of ATP analyses in HBSS and Tyrode’s buffer were analyzed by adding standard ATP (0.1μM or 1μM) into reaction system (E and F). Data are from three independent experiments. At last, the platelets were added into 96-well plate or 384-well plate, and then stimulated with thrombin or collagen at 37°C for 20 min. Bioluminescence analysis of ATP released by activated platelets was initiated by adding luciferase-luciferin work solution containing CoA and BSA at optimized concentrations into 96-well plate and RLU values were determined at 5 min after the start of the reaction (G and H). Concentrations of released ATP and Z’ factors were calculated. Data are from five independent experiments. * P < 0.05 vs Control.

Screening of platelet inhibitors with optimized bioluminescence analysis of released ATP

On the basis of optimized bioluminescence analysis of released ATP, we subsequently analyzed the inhibitory effects of Ly294002 and Staurosporine on the ATP releases of activated platelets and evaluated the application values of bioluminescence analysis of ATP in screening of platelet inhibitors. As shown in Fig 6, both Ly294002 and Staurosporine exhibited inhibitory effects on ATP releases of activated platelets induced by thrombin and they showed similar inhibitory effects in HBSS and Tyrode’s buffer. In addition, it should be noted that the maximum concentration of DMSO in the assay was 0.5% and the effects of DMSO on the platelet analysis was negligible (S1 Fig).

Fig. 6. Screening of platelet inhibitors with optimized bioluminescence analysis of released ATP.
Screening of platelet inhibitors with optimized bioluminescence analysis of released ATP.
Washed platelets resuspended in HBSS or Tyrode’s buffer were seeded in white 96-well plate. Subsequently, the platelets were pretreated with Ly294002 or Staurosporine for 5 min and then stimulated with thrombin at 37°C for 20 min. Bioluminescence analyses of ATP released by activated platelets were performed. Inhibition rates and IC50 of Ly294002 (A and B) or Staurosporine (C and D) on ATP release of activated platelets were calculated. Data are representative of three independent experiments.

Discussion

In 1958, CoA was reported to be able to enhance bioluminescence catalyzed by luciferase and the underlying mechanism was considered to be that CoA could remove inhibitory product from enzyme surface and strengthen enzyme reaction [11]. Afterwards, the inhibitory side product in bioluminescence reaction catalyzed by luciferase was identified to be L-AMP and CoA was revealed to induce stabilization of light emission due to the thiolytic reaction between CoA and L-AMP, which give rise to dehydroluciferyl-CoA (L-CoA), a much less power luciferase inhibitor (IC50 = 5 μM) compared with L-AMP [12–14]. Thus, some commercial luciferase reporter assay kits have used CoA to enhance bioluminescence and stabilize light emission in quantitative analysis of luciferase. However, CoA hasn’t been used in bioluminescence analysis of ATP.

In the present study, we firstly performed standard curve assay of ATP in buffer A which contained tricine buffer of pH 7.8 and high concentration meganesium ions, and endowed luciferase suitable catalytic reaction environment. The linear regression analysis showed that standard curve of ATP assay had unsatisfactory linearity. This bad linearity was mainly resulted from low bioluminescence signals when ATP was at high concentrations. After probable effects of luciferase deficiency were eliminated, we proposed that this bad linearity of ATP analysis was originated by inhibitory effects of L-AMP on enzyme reaction. Thereafter, we investigated the effects of CoA on the bioluminescence analysis of ATP and significantly improved standard curve linearity by adding optimized concentration of CoA in the reaction system. However, signal attenuation of ATP analysis was still very fast even in the presence of CoA. This was because signal attenuation of ATP analysis was mainly resulted from consumption of ATP in the catalyzed reaction but not inhibitory effects of L-AMP on enzyme activity.

Generally, maintenance of platelet activity needs suitable buffer and Tyrode’s buffer is often used to determine activation, release, and aggregation of platelets [1–3]. Thus, we furtherly evaluated bioluminescence analysis of ATP in Tyrode’s buffer and HBSS which also possessed glucose and calcium for platelet activation and magnesium for bioluminescence reaction. The results showed that bioluminescence signals of ATP analyses in HBSS and Tyrode’s buffer were lower than that in buffer A, which suggesting that HBSS or Tyrode’s buffer doesn’t provide best reaction conditions for luciferase. The reasons may be relatively low pH values and magnesium ion concentrations in HBSS and Tyrode’s buffer. However, adding CoA in reaction system also significantly improved linearity of ATP analysis in HBSS and Tyrode’s buffer which is consistent with assay in buffer A. So HBSS and Tyrode’s buffer can be used to determine ATP release of platelets by bioluminescence analysis. Of course, there is still the problem of bioluminescence signal attenuation in HBSS and Tyrode’s buffer.

In some experiments, we occasionally found that signal attenuation of ATP analysis was retarded by adding 0.35% BSA in the Tyrode’s buffer for reducing liquid shear force on platelets. Meanwhile, signal intensity of bioluminescence was enhanced by 0.35% BSA. The underlying mechanisms of enhancing bioluminescence signal and postponing signal attenuation in ATP analysis are not clear. As a macromolecule biochemical reagent, BSA is often used to prevent denaturation of many enzymes and cytokines. In some luciferase reporter kit, BSA also used to hinder adsorption of luciferase on multi-well plate and thus keep activity of luciferase. However, we proposed that BSA could play much more meaningful roles in bioluminescence analysis of ATP by slowing down signal attenuation and improving accuracy. Through concentration optimization analysis, we furtherly revealed that mitigation effects of BSA on signal attenuation of bioluminescence were proportional to its concentration and the biggest enhancement effects of BSA on bioluminescence signal were achieved by 0.0312% to 0.125% BSA in HBSS and Tyrode’s buffer. So we used 0.05% BSA to slow down signal attenuation of ATP analysis in later experiments. Based on these results, we also propose a hypothesis that BSA can enhance luminous efficiency of bioluminescence reaction catalyzed by luciferase as done by some cationic liposomes [15, 16] and reduce reaction rate simultaneously. Reduction of bioluminescence reaction rate slows down consumption of ATP and corresponding signal attenuation of bioluminescence while signal intensity is maintained and even enhanced by higher luminous efficiency in the presence of BSA.

In order to investigate the specificity that BSA enhances luminous efficiency and slows down signal attenuation in bioluminescence analysis of ATP, we furtherly evaluated the effects of BSA on chemiluminescence reaction in HRP analysis [17] and the effects of OVA on bioluminescence reaction of ATP analysis. Interestingly, BSA does not slow down signal attenuation of chemiluminescence analysis of HRP and even lowered signal intensity. However, OVA enhanced bioluminescence intensity and postponed signal attenuation in bioluminescence analysis of ATP which was consistent with the effects of BSA. So BSA possesses high specificity in enhancing luminous efficiency and slowing down signal attenuation in bioluminescence analysis of ATP. This specificity is not resulted from preventing denaturation and inactivation of luciferase as usual, but from enhancing luminous efficiency and slowing down bioluminescence reaction rate simultaneously. Especially, the common enhancing effects of BSA and OVA on enhancing luminous efficiency may be contributed by some common amino acid residues. According to report made by Airth et al., cysteine can probably react with L-CoA to produce more stable L-cysteine and furtherly enhance bioluminescence of ATP analysis [11]. Thus the mitigation effects of BSA and OVA on signal attenuation in bioluminescence analysis of ATP may be resulted from united effects of enhancing luminous efficiency by cysteine residues and slowing down bioluminescence reaction rate by barrier effects of BSA and OVA as biological macromolecules. In subsequent platelet experiments, we used CoA to improve linearity and BSA to slow down signal attenuation simultaneously.

Platelet secretion occurs upon activation by specific agonists including thrombin, collagen, ADP, and thromboxane. Previous studies have fully demonstrated that ATP was released from dense granules after thrombin and collagen induced platelet activation [1–3]. In the present study, we further demonstrated that in the presence of platelets, bioluminescence analysis of ATP was significantly optimized by addition of CoA and BSA. Thus, the recovery rates of ATP analyses in HBSS and Tyrode’s buffer were significantly improved by optimized method. Subsequently, we validated that platelets could be activated to release ATP by thrombin and collagen, and bioluminescence analysis of ATP in the presences of CoA and BSA could accurately determine ATP release. No matter in HBSS or Tyrode’s buffer the assays were performed, the Z’ values of ATP analyses were all over 0.5. These results suggest that the optimized bioluminescence analysis of ATP might be potential to be used to screen platelet inhibitors with high throughput. It should be noted that this assay couldn’t be performed in PRP, because PRP would coagulate after stimulated by thrombin and platelets in PRP intensely aggregate after treated with collagen I, then interfering the analysis of ATP release.

Platelet agonists induce activation and aggregation of platelets and following thrombus formation through stimulating multiple signaling pathways [18–25]. After optimized bioluminescence analysis of ATP and evaluated its availability in ATP release analysis of platelets, we validated its application values in the high throughput screening of platelet inhibitors by analyzing inhibitory effects of PI3K inhibitor and PKC inhibitor on ATP release of platelets. Consistent with expectation, inhibitory effects of PI3K inhibitor and PKC inhibitor on ATP release of platelets induced by thrombin were perfectly determined by optimized bioluminescence analysis of ATP. PI3K inhibitor or PKC inhibitor had similar IC50 values on ATP releases of platelets in HBSS and Tyrode’s buffer.

Collectively, we have successfully optimized bioluminescence analysis of ATP by improving standard curve linearity with CoA and postponing signal attenuation with BSA. This optimized method can be used to accurately determine activation of platelets and screen platelet inhibitors with high throughput in HBSS or Tyrode’s buffer.

Supporting information

S1 Fig [tif]
The effects of DMSO on ATP release of platelets.

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Článek Multi-disciplinary interventions for chronic pain involving education: A systematic review
Článek Efficacy of Lactobacillus-supplemented triple therapy for H. pylori eradication: A meta-analysis of randomized controlled trials
Článek Testing gene by community disadvantage moderation of sexual health outcomes among urban women
Článek The cost of illness and economic burden of endometriosis and chronic pelvic pain in Australia: A national online survey
Článek A real-time gesture recognition system using near-infrared imagery
Článek Alcohol use and its association with sexual risk behaviors in the Armed Forces of the Republic of the Congo
Článek Improved survival rates in patients with H1N1 acute respiratory failure in Korea between 2009 and 2016
Článek Electroencephalographic features of discontinuous activity in anesthetized infants and children
Článek Tumor stromal type is associated with stromal PD-L1 expression and predicts outcomes in breast cancer
Článek Mutant prevention concentration of ozenoxacin for quinolone-susceptible or -resistant Staphylococcus aureus and Staphylococcus epidermidis
Článek The Echobot: An automated system for stimulus presentation in studies of human echolocation
Článek Magnetic resonance imaging presentation of deep infiltrating endometriosis nodules before and after pregnancy: A case series
Článek Utilizing multiplex fluor LAMPs to illuminate multiple gene expressions in situ
Článek Genetic relationships between the RACK1 homolog cpc-2 and heterotrimeric G protein subunit genes in Neurospora crassa
Článek Is latent tuberculosis infection challenging in Iranian health care workers? A systematic review and meta-analysis
Článek A comparison of the risk of congestive heart failure-related hospitalizations in patients receiving hemodialysis and peritoneal dialysis - A retrospective propensity score-matched study
Článek A tailored approach to fusion transcript identification increases diagnosis of rare inherited disease
Článek Experiences, needs, and preferences for follow-up after stroke perceived by people with stroke and healthcare professionals: A focus group study
Článek The Nordic Maintenance Care Program: Does psychological profile modify the treatment effect of a preventive manual therapy intervention? A secondary analysis of a pragmatic randomized controlled trial
Článek Motivation for alcohol consumption or abstinence during pregnancy: A clinical-qualitative study in Brazil
Článek Physical fitness and physical self-concept of male and female young adults in Qatar
Článek Improved semi-supervised autoencoder for deception detection
Článek ProphET, prophage estimation tool: A stand-alone prophage sequence prediction tool with self-updating reference database
Článek Time scale of resilience loss: Implications for managing critical transitions in water quality
Článek Effectiveness of physical and cognitive-behavioural intervention programmes for chronic musculoskeletal pain in adults: A systematic review and meta-analysis of randomised controlled trials
Článek Relationship between feed efficiency indexes and performance, body measurements, digestibility, energy partitioning, and nitrogen partitioning in pre-weaning dairy heifers
Článek Long-stay patients in pediatric intensive care unit: Diagnostic-specific definition and predictors
Článek Whole genome sequencing to identify predictive markers for the risk of drug-induced interstitial lung disease
Článek A systematic review and thematic synthesis of Canada’s LGBTQ2S+ employment, labour market and earnings literature
Článek Analysis of the protein composition of the spindle pole body during sporulation in Ashbya gossypii
Článek The views and experiences of general dental practitioners (GDP’s) in West Yorkshire who used the International Caries Detection and Assessment System (ICDAS) in research
Článek Compare HIV/syphilis infections between age groups and explore associated factors of HIV/syphilis co-infections among men who have sex with men in Shenzhen, China, from 2009 to 2017
Článek Ceftiofur formulation differentially affects the intestinal drug concentration, resistance of fecal Escherichia coli, and the microbiome of steers
Článek Effect of exclusive breastfeeding cessation time on childhood morbidity and adverse nutritional outcomes in Ethiopia: Analysis of the demographic and health surveys
Článek Dual antiplatelet therapy in patients with cirrhosis and acute myocardial infarction – A 13-year nationwide cohort study
Článek Relationships between electrolyte and amino acid compositions in sweat during exercise suggest a role for amino acids and K+ in reabsorption of Na+ and Cl- from sweat
Článek Management and glycemic control of patients with type 2 diabetes mellitus at primary care level in Kedah, Malaysia: A statewide evaluation
Článek Factors associated with unsafe abortion practices in Nepal: Pooled analysis of the 2011 and 2016 Nepal Demographic and Health Surveys
Článek What has reproductive health decision-making capacity got to do with unintended pregnancy? Evidence from the 2014 Ghana Demographic and Health Survey
Článek Circulating levels of CD34+ cells predict long-term cardiovascular outcomes in patients on maintenance hemodialysis
Článek Penetration of linezolid into bone tissue 24 h after administration in patients with multidrug-resistant spinal tuberculosis
Článek Prosthesis design of animal models of periprosthetic joint infection following total knee arthroplasty: A systematic review
Článek Coherent diversification in corporate technological portfolios
Článek Effect of tranexamic acid administration on acute traumatic coagulopathy in rats with polytrauma and hemorrhage
Článek Factors associated with the use of diet and the use of exercise for prostate cancer by long-term survivors
Článek Congenital Zika Syndrome in a Brazil-Paraguay-Bolivia border region: Clinical features of cases diagnosed between 2015 and 2018
Článek Trends and impact of antimicrobial resistance on older inpatients with urinary tract infections (UTIs): A national retrospective observational study
Článek Malawian children with uncomplicated and cerebral malaria have decreased activated Vγ9Vδ2 γδ T cells which increase in convalescence
Článek Predictors of mortality within the first year of initiating antiretroviral therapy in urban and rural Kenya: A prospective cohort study
Článek The investment case as a mechanism for addressing the NCD burden: Evaluating the NCD institutional context in Jamaica, and the return on investment of select interventions
Článek Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis
Článek Gravity influences bevacizumab distribution in an undisturbed balanced salt solution in vitro
Článek Proteomic changes of aryl hydrocarbon receptor (AhR)-silenced porcine granulosa cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
Článek Improving the forecasting performance of temporal hierarchies
Článek Prevalence of surgically correctable conditions among children in a mixed urban-rural community in Nigeria using the SOSAS survey tool: Implications for paediatric surgical capacity-building
Článek The impact of PEPFAR transition on HIV service delivery at health facilities in Uganda
Článek Impact of peri-intraventricular haemorrhage and periventricular leukomalacia in the neurodevelopment of preterms: A systematic review and meta-analysis
Článek Correction: Activation of aryl hydrocarbon receptor signaling by a novel agonist ameliorates autoimmune encephalomyelitis
Článek Macrophage PPAR-γ suppresses long-term lung fibrotic sequelae following acute influenza infection
Článek Determination of factors affecting medication adherence in type 2 diabetes mellitus patients using a nationwide claim-based database in Japan
Článek Clade II Candida auris possess genomic structural variations related to an ancestral strain
Článek Effect of aging on the association between ankle muscle strength and the control of bipedal stance
Článek Dietary polyphenols as a safe and novel intervention for modulating pain associated with intervertebral disc degeneration in an in-vivo rat model
Článek Comparison of transcriptomes of an orthotospovirus vector and non-vector thrips species
Článek Whole genome resequencing of watermelons to identify single nucleotide polymorphisms related to flesh color and lycopene content
Článek Religious practices and long-term survival after hospital discharge for an acute coronary syndrome
Článek Behavioral recovery and spinal motoneuron remodeling after polyethylene glycol fusion repair of singly cut and ablated sciatic nerves
Článek Platelets modulate multiple markers of neutrophil function in response to in vitro Toll-like receptor stimulation
Článek Temperature sensitivity patterns of carbon and nitrogen processes in decomposition of boreal organic soils – Quantification in different compounds and molecule sizes based on a multifactorial experiment
Článek Cows painted with zebra-like striping can avoid biting fly attack
Článek Botulinum toxin A injection into the entopeduncular nucleus improves dynamic locomotory parameters in hemiparkinsonian rats
Článek Influence of mydriasis on optical coherence tomography angiography imaging in patients with age-related macular degeneration
Článek Association between plural legal systems and sexual and reproductive health outcomes for women and girls in Nigeria: A state-level ecological study
Článek Utility of qSOFA and modified SOFA in severe malaria presenting as sepsis
Článek Correction: Perspectives of general dental practitioners on preventive, patient-centred, and evidence-based oral healthcare—A Q-methodology study
Článek No evidence of associations between ADHD and event-related brain potentials from a continuous performance task in a population-based sample of adolescent twins
Článek Antibiotic resistance of blood cultures in regional and tertiary hospital settings of Tyrol, Austria (2006-2015): Impacts & trends
Článek Cell-free DNA levels of twins and sibling pairs indicate individuality and possible use as a personalized biomarker
Článek Catastrophic factors involved in road accidents: Underlying causes and descriptive analysis
Článek Detection and determination of stability of the antibiotic residues in cow’s milk
Článek Neural minimization methods (NMM) for solving variable order fractional delay differential equations (FDDEs) with simulated annealing (SA)
Článek Maternal and perinatal outcomes among women with hypertensive disorders in pregnancy in Kumasi, Ghana
Článek Characteristics and costs in adults with acute poisoning admitted to the emergency department of a university hospital in Belgium
Článek War pact model of shrinking networks
Článek Treatment outcomes based on radiation therapy fields for bifocal germinoma: Synchronous or disseminated disease?
Článek Effect of an intensive 3-day social cognitive treatment (can do treatment) on control self-efficacy in patients with relapsing remitting multiple sclerosis and low disability: A single-centre randomized controlled trial
Článek Successful development of methodology for detection of hapten-specific contact hypersensitivity (CHS) memory in swine
Článek Postural changes in optic nerve and optic nerve sheath diameters in postural orthostatic tachycardia syndrome and spontaneous intracranial hypotension: A cohort study
Článek Attitudes and referral practices for pre-exposure prophylaxis (PrEP) among HIV rapid testers and case managers in Philadelphia: A mixed methods study
Článek The landscape for HIV pre-exposure prophylaxis during pregnancy and breastfeeding in Malawi and Zambia: A qualitative study
Článek Frozen by stress: Stress increases scope insensitivity
Článek Infant dietary intake of yogurt and cheese and gastroenteritis at 1 year of age: The Japan Environment and Children’s Study
Článek Attenuation of renal fibrosis after unilateral ureteral obstruction in mice lacking the N-type calcium channel
Článek Anesthesia during deployment of a military forward surgical unit in low income countries: A register study of 1547 anesthesia cases
Článek CsBZIP40, a BZIP transcription factor in sweet orange, plays a positive regulatory role in citrus bacterial canker response and tolerance
Článek Differences in perceived popularity and social preference between bullying roles and class norms
Článek Identity and pathogenicity of some fungi associated with hazelnut (Corylus avellana L.) trunk cankers in Oregon
Článek Associations between focus constructions and levels of exhaustivity: An experimental investigation of Chinese
Článek Give science and peace a chance: Speeches by Nobel laureates in the sciences, 1901-2018
Článek Adaptation of H3N2 canine influenza virus to feline cell culture
Článek Isoflurane mediated neuropathological and cognitive impairments in the triple transgenic Alzheimer’s mouse model are associated with hippocampal synaptic deficits in an age-dependent manner
Článek Does antibiotic use accelerate or retard cutaneous repair? A systematic review in animal models
Článek Diagnostic accuracy of midkine on hepatocellular carcinoma: A meta-analysis
Článek Grouping of complex substances using analytical chemistry data: A framework for quantitative evaluation and visualization
Článek A cross sectional comparison of drug use indicators using WHO methodology in primary level hospitals participating in an Auditable Pharmaceutical Transactions and Services program versus non-APTS primary hospitals in Southern Ethiopia
Článek Liquid biopsies for omics-based analysis in sentinel mussels
Článek Reporting biases in self-assessed physical and cognitive health status of older Europeans
Článek Trends in the characteristics of acute primary angle closure in Korea over the past 10-years
Článek Obesity-associated insulin resistance adversely affects skin function
Článek First-line treatments in EGFR-mutated advanced non-small cell lung cancer: A network meta-analysis
Článek Factors influencing the behaviour and perceptions of Australian veterinarians towards antibiotic use and antimicrobial resistance
Článek Cognitive, relational and task crafting: Spanish adaptation and analysis of psychometric properties of the Job Crafting Questionnaire
Článek Joint emulation of Earth System Model temperature-precipitation realizations with internal variability and space-time and cross-variable correlation: fldgen v2.0 software description
Článek Correction: Low dose naltrexone: Effects on medication in rheumatoid and seropositive arthritis. A nationwide register-based controlled quasi-experimental before-after study
Článek Correction: Human indole(ethyl)amine-N-methyltransferase (hINMT) catalyzed methylation of tryptamine, dimethylsulfide and dimethylselenide is enhanced under reducing conditions - A comparison between 254C and 254F, two common hINMT variants
Článek Myofibrillar function differs markedly between denervated and dexamethasone-treated rat skeletal muscles: Role of mechanical load
Článek A proteomic clock for malignant gliomas: The role of the environment in tumorigenesis at the presymptomatic stage
Článek Regional, institutional, and departmental factors associated with gender diversity among BS-level chemical and electrical engineering graduates
Článek Transepithelial transport of P-glycoprotein substrate by the Malpighian tubules of the desert locust
Článek Sex affects immunolabeling for histone 3 K27me3 in the trophectoderm of the bovine blastocyst but not labeling for histone 3 K18ac
Článek Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity
Článek The motility-based swim-up technique separates bull sperm based on differences in metabolic rates and tail length
Článek Synbiotic supplementation to decrease Salmonella colonization in the intestine and carcass contamination in broiler birds
Článek Bexarotene therapy ameliorates behavioral deficits and induces functional and molecular changes in very-old Triple Transgenic Mice model of Alzheimer´s disease
Článek States with higher minimum wages have lower STI rates among women: Results of an ecological study of 66 US metropolitan areas, 2003-2015
Článek Weakening the subjective sensation of own hand ownership does not interfere with rapid finger movements
Článek Identification of DNA methyltransferases and demethylases in Solanum melongena L., and their transcription dynamics during fruit development and after salt and drought stresses
Článek Environmental conditions for Jamestown Canyon virus correlated with population-level resource selection by white-tailed deer in a suburban landscape
Článek Particularly neglected in countries with other challenges: High Toxoplasma gondii seroprevalence in pregnant women in Kabul, Afghanistan, while a low proportion know about the parasite
Článek Vaccination status of patients using anti-TNF therapy and the physicians’ behavior shaping the phenomenon: Mixed-methods approach
Článek Antimicrobial resistance (AMR) and molecular characterization of Neisseria gonorrhoeae in Ghana, 2012-2015
Článek Suppression treatment differentially influences the microbial community and the occurrence of broad host range plasmids in the rhizosphere of the model cover crop Avena sativa L.
Článek Effects of dietary intake and nutritional status on cerebral oxygenation in patients with chronic kidney disease not undergoing dialysis: A cross-sectional study
Článek Micro-RNA 150-5p predicts overt heart failure in patients with univentricular hearts
Článek Flexible learning spaces facilitate interaction, collaboration and behavioural engagement in secondary school
Článek Clinical significance and prognostic role of hypoxia-induced microRNA 382 in gastric adenocarcinoma
Článek Genome-wide investigation of superoxide dismutase (SOD) gene family and their regulatory miRNAs reveal the involvement in abiotic stress and hormone response in tea plant (Camellia sinensis)
Článek Prevalence of Trypanosoma cruzi infection in active military population of The Colombian National Army gathered in five departments
Článek Increased aggression and reduced aversive learning in honey bees exposed to extremely low frequency electromagnetic fields
Článek Is summer food intake a limiting factor for boreal browsers? Diet, temperature, and reproduction as drivers of consumption in female moose
Článek eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization
Článek The interplay of personality and attitudes toward own aging across two decades of later life
Článek Reconstructing the population history of the sandy beach amphipod Haustorioides japonicus using the calibration of demographic transition (CDT) approach
Článek Correction: Blue light-dependent human magnetoreception in geomagnetic food orientation
Článek Re-evaluation of a Tn5::gacA mutant of Pseudomonas syringae pv. tomato DC3000 uncovers roles for uvrC and anmK in promoting virulence
Článek Sugar-sweetened beverages and colorectal cancer risk in the California Teachers Study
Článek Illuminating biological pathways for drug targeting in head and neck squamous cell carcinoma
Článek Effects of insecticides, fipronil and imidacloprid, on the growth, survival, and behavior of brown shrimp Farfantepenaeus aztecus
Článek Prevalence of pectus excavatum (PE), pectus carinatum (PC), tracheal hypoplasia, thoracic spine deformities and lateral heart displacement in thoracic radiographs of screw-tailed brachycephalic dogs
Článek Validation of the vignette-based German Exercise Causality Orientation Scale (G-ECOS)
Článek Hierarchical classification of snowmelt episodes in the Pyrenees using seismic data
Článek A national survey of videolaryngoscopes and alternative intubation devices in Hungary
Článek The relevance of U.S. Strategic Highway Safety Plans in a future context
Článek Anti-inflammatory effects induced by ultralow concentrations of bupivacaine in combination with ultralow concentrations of sildenafil (Viagra) and vitamin D3 on inflammatory reactive brain astrocytes
Článek The cost-effectiveness of controlling cervical cancer using a new 9-valent human papillomavirus vaccine among school-aged girls in Australia
Článek Inactivation of chronic wasting disease prions using sodium hypochlorite
Článek Short-term effects of video gaming on brain response during working memory performance
Článek Insights into the behavior of six rationally designed peptides based on Escherichia coli’s OmpA at the water-dodecane interface
Článek Identification of muscle-specific candidate genes in Simmental beef cattle using imputed next generation sequencing
Článek A game theoretic setting of capitation versus fee-for-service payment systems
Článek In utero exposure to economic fluctuations and birth outcomes: An analysis of the relevance of the local unemployment rate in Brazilian state capitals
Článek Evaluation of left ventricular dimension and systolic function by standard transthoracic echocardiography before and 24-hours after percutaneous closure of patent ductus arteriosus in 120 dogs
Článek Correction: Homology analysis of malware based on ensemble learning and multifeatures
Článek Extended-wavelength diffuse reflectance spectroscopy with a machine-learning method for in vivo tissue classification
Článek Development of a recombinant replication-deficient rabies virus-based bivalent-vaccine against MERS-CoV and rabies virus and its humoral immunogenicity in mice
Článek Educational achievement at age 9.5 years of children born to mothers maintained on methadone during pregnancy
Článek Correction: Functional atlases for analysis of motor and neuropsychological outcomes after medial globus pallidus and subthalamic stimulation
Článek Correction: Estimating Body Composition in Adolescent Sprint Athletes: Comparison of Different Methods in a 3 Years Longitudinal Design
Článek Intrabladder pressure as predictor of intra-abdominal pressure in horses
Článek Correction: Hafting of Middle Paleolithic tools in Latium (central Italy): New data from Fossellone and Sant’Agostino caves
Článek Isotopic compositions of ground ice in near-surface permafrost in relation to vegetation and microtopography at the Taiga–Tundra boundary in the Indigirka River lowlands, northeastern Siberia
Článek Single-cell qPCR demonstrates that Repsox treatment changes cell fate from endoderm to neuroectoderm and disrupts epithelial-mesenchymal transition
Článek Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
Článek Take one step backward to move forward: Assessment of genetic diversity and population structure of captive Asian woolly-necked storks (Ciconia episcopus)
Článek Inter-method and anatomical correlates of episodic memory tests in the Alzheimer’s Disease spectrum
Článek Correction: Neural computational model GrowthEstimate: A model for studying living resources through digestive efficiency
Článek Genetic diversity and population structure of the Mediterranean sesame core collection with use of genome-wide SNPs developed by double digest RAD-Seq
Článek Can work climate foster pro-environmental behavior inside and outside of the workplace?
Článek Life-course trajectories of body mass index and subsequent cardiovascular risk among Chinese population
Článek Visual inputs decrease brain activity in frontal areas during silent lipreading
Článek Expression of Concern: The Coordination of Cell Growth during Fission Yeast Mating Requires Ras1-GTP Hydrolysis
Článek Does intrauterine crowding affect the force generating capacity and muscle composition of the piglet front limb?
Článek Interactions of Streptococcus suis serotype 9 with host cells and role of the capsular polysaccharide: Comparison with serotypes 2 and 14
Článek A flexible formula for incorporating distributive concerns into cost-effectiveness analyses: Priority weights
Článek Correction: Lack of association between hypothyroxinemia of prematurity and transient thyroid abnormalities with adverse long term neurodevelopmental outcome in very low birth weight infants
Článek Vancomycin-resistance gene cluster, vanC, in the gut microbiome of acute leukemia patients undergoing intensive chemotherapy
Článek Additive effects of a small molecular PCNA inhibitor PCNA-I1S and DNA damaging agents on growth inhibition and DNA damage in prostate and lung cancer cells
Článek Correction: BCG vaccination and tuberculosis prevention: A forty years cohort study, Monastir, Tunisia
Článek Correction: Perceptions among transgender women of factors associated with the access to HIV/AIDS-related health services in Yogyakarta, Indonesia
Článek Correction: Silymarin in non-cirrhotics with non-alcoholic steatohepatitis: A randomized, double-blind, placebo controlled trial
Článek Correction: Anatomical analysis of antebrachial cutaneous nerve distribution pattern and its clinical implications for sensory reconstruction
Článek Correction: Comparison of risk models for mortality and cardiovascular events between machine learning and conventional logistic regression analysis
Článek Correction: Genetic variants linked to myopic macular degeneration in persons with high myopia: CREAM Consortium
Článek Correction: Increased proliferation and altered cell cycle regulation in pancreatic stem cells derived from patients with congenital hyperinsulinism
Článek Correction: Retraction: Stiffening-Induced High Pulsatility Flow Activates Endothelial Inflammation via a TLR2/NF-κB Pathway
Článek Development of a universal short patient satisfaction questionnaire on the basis of SERVQUAL: Psychometric analyses with data of diabetes and stroke patients from six different European countries
Článek The effect of heavy metal contamination on humans and animals in the vicinity of a zinc smelting facility
Článek Characterization and variation of the rhizosphere fungal community structure of cultivated tetraploid cotton
Článek Clinical experiences with the use of oxytocin injection by healthcare providers in a southwestern state of Nigeria: A cross-sectional study
Článek The psychometric validation of the Dutch version of the Rivermead Post-Concussion Symptoms Questionnaire (RPQ) after traumatic brain injury (TBI)
Článek Population extinctions driven by climate change, population size, and time since observation may make rare species databases inaccurate
Článek A configural model of expert judgement as a preliminary epidemiological study of injury problems: An application to drowning
Článek Economic burden of hypoglycemia for type II diabetes mellitus patients in Malaysia
Článek Phenotypic determinism and contingency in the evolution of hypothetical tree-like organisms
Článek Predicting diabetes second-line therapy initiation in the Australian population via time span-guided neural attention network
Článek Tobacco use and associated factors among Rwandan youth aged 15-34 years: Findings from a nationwide survey, 2013
Článek Disease spectrum and outcomes among elderly patients in two tertiary hospitals in Dar es Salaam, Tanzania
Článek LRRC33 is a novel binding and potential regulating protein of TGF-β1 function in human acute myeloid leukemia cells
Článek No evidence for kin recognition in a passerine bird
Článek The ineligibility of food products from across the EU for marketing to children according to two EU-level nutrient profile models
Článek The Ps and Qs of alarmone synthesis in Staphylococcus aureus
Článek Assessing introgressive hybridization in roan antelope (Hippotragus equinus): Lessons from South Africa
Článek Robust sound event detection in bioacoustic sensor networks
Článek DNA methylation, microRNA expression profiles and their relationships with transcriptome in grass-fed and grain-fed Angus cattle rumen tissue
Článek Facile assembly of an affordable miniature multicolor fluorescence microscope made of 3D-printed parts enables detection of single cells
Článek The inhibitory effects of butein on cell proliferation and TNF-α-induced CCL2 release in racially different triple negative breast cancer cells
Článek The long-run effects of secondary school track assignment
Článek Measuring the impact of an interdisciplinary learning project on nursing, architecture and landscape design students’ empathy
Článek Characterising the harmonic vocal repertoire of the Indian wolf (Canis lupus pallipes)
Článek Childhood conditions, pathways to entertainment work and current practices of female entertainment workers in Cambodia: Baseline findings from the Mobile Link trial
Článek Adoption and consequences of new light-fishing technology (LEDs) on Lake Tanganyika, East Africa
Článek An innovative non-invasive technique for subcutaneous tumour measurements
Článek Stabilization of α-synuclein oligomers using formaldehyde
Článek cgmanalysis: An R package for descriptive analysis of continuous glucose monitor data
Článek Simple methods to obtain food listing and portion size distribution estimates for use in semi-quantitative dietary assessment methods
Článek A multi-level society comprised of one-male and multi-male core units in an African colobine (Colobus angolensis ruwenzorii)
Článek Development of rice conidiation media for Ustilaginoidea virens
Článek Thymically-derived Foxp3+ regulatory T cells are the primary regulators of type 1 diabetes in the non-obese diabetic mouse model
Článek Algorithmic differentiation improves the computational efficiency of OpenSim-based trajectory optimization of human movement
Článek Male long-distance migrant turned sedentary; The West European pond bat (Myotis dasycneme) alters their migration and hibernation behaviour
Článek Spectral measure of color variation of black-orange-black (BOB) pattern in small parasitoid wasps (Hymenoptera: Scelionidae), a statistical approach
Článek RFQAmodel: Random Forest Quality Assessment to identify a predicted protein structure in the correct fold
Článek Perceived barriers in accessing sexual and reproductive health services for youth in Lao People’s Democratic Republic
Článek Armadillo repeat-containing protein 1 is a dual localization protein associated with mitochondrial intermembrane space bridging complex
Článek Cost utility analysis of end stage renal disease treatment in Ministry of Health dialysis centres, Malaysia: Hemodialysis versus continuous ambulatory peritoneal dialysis
Článek Diversity and life-cycle analysis of Pacific Ocean zooplankton by videomicroscopy and DNA barcoding: Hydrozoa
Článek Chronic stability of single-channel neurophysiological correlates of gross and fine reaching movements in the rat
Článek Neural, functional, and aesthetic impacts of spatially heterogeneous flicker: A potential role of natural flicker
Článek Clinical and laboratorial profiles of dengue virus infection in kidney transplant recipients: Report of a single center
Článek Bioaccumulation of heavy metals in some commercially important fishes from a tropical river estuary suggests higher potential health risk in children than adults
Článek Factors associated with unsuppressed viremia in women living with HIV on lifelong ART in the multi-country US-PEPFAR PROMOTE study: A cross-sectional analysis
Článek Comparing low-cost handheld autorefractors: A practical approach to measuring refraction in low-resource settings
Článek Tissue-specific changes in size and shape of the ligaments and tendons of the porcine knee during post-natal growth
Článek Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation
Článek Differentiating electrophysiological indices of internal and external performance monitoring: Relationship with perfectionism and locus of control
Článek The N125S polymorphism in the cathepsin G gene (rs45567233) is associated with susceptibility to osteomyelitis in a Spanish population
Článek Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39
Článek Bayesian inverse methods for spatiotemporal characterization of gastric electrical activity from cutaneous multi-electrode recordings
Článek Evolutionary history and classification of Micropia retroelements in Drosophilidae species
Článek Low-glutathione mutants are impaired in growth but do not show an increased sensitivity to moderate water deficit
Článek Exploring the basis of 2-propenyl and 3-butenyl glucosinolate synthesis by QTL mapping and RNA-sequencing in Brassica juncea
Článek Risk factor profiles and clinical outcomes for children and adults with pneumococcal infections in Singapore: A need to expand vaccination policy?
Článek The effect of yoga practice on glycemic control and other health parameters in the prediabetic state: A systematic review and meta-analysis
Článek Decreased breast cancer-specific mortality risk in patients with a history of thyroid cancer
Článek Intestinal parasitic infection among household contacts of primary cases, a comparative cross-sectional study
Článek An automatic approach for classification and categorisation of lip morphological traits
Článek Time series analysis and forecasting with ECOTOOL
Článek OCT-Angiography: Mydriatic phenylephrine and tropicamide do not influence retinal microvasculature in macula and peripapillary region
Článek Azithromycin non-susceptible Shigella circulating in Israel, 2014–2016
Článek The effectiveness of a community-based, type 2 diabetes prevention programme on health-related quality of life. The DE-PLAN study
Článek Brain metastasis-related microRNAs in patients with advanced breast cancer
Článek Empathy affects tradeoffs between life's quality and duration
Článek Novel approaches to model effects of subconjunctival blebs on flow pressure to improve clinical grading systems after glaucoma drainage surgery
Článek Global incidence of prostate cancer in developing and developed countries with changing age structures
Článek Variation in bone response to the placement of percutaneous osseointegrated endoprostheses: A 24-month follow-up in sheep
Článek Preventable pediatric hospitalizations and access to primary health care in Italy
Článek A system for bedside assistance that integrates a robotic bed and a mobile manipulator
Článek A global sampler of single particle tracking solutions for single molecule microscopy
Článek Multi-breed genome-wide association studies across countries for electronically recorded behavior traits in local dual-purpose cows
Článek Sensitivity to changes in dynamic affordances for walking on land and at sea
Článek Dombi power partitioned Heronian mean operators of q-rung orthopair fuzzy numbers for multiple attribute group decision making
Článek Impact of visual impairment on physical activity in early and late age-related macular degeneration
Článek A fuzzy logic decision support model for climate-driven biomass loss risk in western Oregon and Washington
Článek Investigating the potential of the secretome of mesenchymal stem cells derived from sickle cell disease patients
Článek Quantitative assessment of plant-arthropod interactions in forest canopies: A plot-based approach
Článek Cryptic diversity of limestone karst inhabiting land snails (Cyclophorus spp.) in northern Vietnam, their evolutionary history and the description of four new species
Článek Using AST-platelet ratio index and fibrosis 4 index for detecting chronic hepatitis C in a large-scale community screening
Článek Elderly HIV-positive women: A gender-based analysis from the Multicenter Italian “GEPPO” Cohort
Článek Attitudes and perceptions towards hypoglycaemia in patients with diabetes mellitus: A multinational cross-sectional study
Článek Incorporating evaporative water loss into bioenergetic models of hibernation to test for relative influence of host and pathogen traits on white-nose syndrome
Článek Relationship of the lobular branch of the great auricular nerve to the tympanoparotid fascia: Spatial anatomy for salvage during face and neck lift
Článek Systematic review and REMARK scoring of renal cell carcinoma prognostic circulating biomarker manuscripts
Článek Assessing the potential of plains zebra to maintain African horse sickness in the Western Cape Province, South Africa
Článek Age, body weight and ovarian function affect oocyte size and morphology in non-PCOS patients undergoing intracytoplasmic sperm injection (ICSI)
Článek Nutrigenomic effect of conjugated linoleic acid on growth and meat quality indices of growing rabbit
Článek Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences
Článek Assessment of community knowledge, practice, and determinants of malaria case households in the rural area of Raya Azebo district, Northern Ethiopia, 2017
Článek Leadership in informal stormwater governance networks
Článek Are dialects socially learned in marmoset monkeys? Evidence from translocation experiments
Článek Using remote sensing to detect whale strandings in remote areas: The case of sei whales mass mortality in Chilean Patagonia
Článek Class III β-tubulin expression as a predictor of docetaxel-resistance in metastatic castration-resistant prostate cancer
Článek Quantitative assessment of acetabular bone defects: A study of 50 computed tomography data sets
Článek HaTSPiL: A modular pipeline for high-throughput sequencing data analysis
Článek Pathogen surveillance in the informal settlement, Kibera, Kenya, using a metagenomics approach
Článek African-American and Caucasian participation in postmortem human brain donation for neuropsychiatric research
Článek Two-dimensional fruit quality factors and soil nutrients reveals more favorable topographic plantation of Xinjiang jujubes in China
Článek The burden of oral conditions among adolescents living with HIV at a clinic in Johannesburg, South Africa
Článek The impact of peer pressure on cigarette smoking among high school and university students in Ethiopia: A systemic review and meta-analysis
Článek Strategies for the hypothermic preservation of cell sheets of human adipose stem cells
Článek Social media as a data resource for #monkseal conservation
Článek Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains
Článek Reading-out task variables as a low-dimensional reconstruction of neural spike trains in single trials
Článek ‘Fat’s chances’: Loci for phenotypic dispersion in plasma leptin in mouse models of diabetes mellitus
Článek Functional models in genome-wide selection
Článek ESLI: Enhancing slope one recommendation through local information embedding
Článek Projected urban growth in the southeastern USA puts small streams at risk
Článek Adverse prognosis of glioblastoma contacting the subventricular zone: Biological correlates
Článek Widespread chytrid infection across frogs in the Peruvian Amazon suggests critical role for low elevation in pathogen spread and persistence
Článek Enriched taxa were found among the gut microbiota of centenarians in East China
Článek Differences in energy and nutritional content of menu items served by popular UK chain restaurants with versus without voluntary menu labelling: A cross-sectional study
Článek Evaluation of date palm pollen (Phoenix dactylifera L.) encapsulation, impact on the nutritional and functional properties of fortified yoghurt
Článek Subgenotyping and genetic variability of hepatitis C virus in Palestine
Článek Measuring sustainability of seed-funded earth science informatics projects
Článek Assessing the role of toll-like receptor in isolated, standard and enriched housing conditions
Článek Declared impact of the US President’s statements and campaign statements on Latino populations’ perceptions of safety and emergency care access
Článek Pathophysiological role of prostanoids in coagulation of the portal venous system in liver cirrhosis
Článek Macular thickness measurements of healthy, naïve cynomolgus monkeys assessed with spectral-domain optical coherence tomography (SD-OCT)
Článek Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
Článek Forma mentis networks quantify crucial differences in STEM perception between students and experts
Článek A population-based cohort study examining the association of documented bladder diverticulum and bladder cancer risk in urology patients
Článek Health assessment of Conolophus subcristatus, Conolophus pallidus, and C. subcristatus X Amblyrhynchus cristatus hybrid (Galápagos land iguanas)
Článek Prognostic value of post-induction chemotherapy 18F-FDG PET-CT in stage II/III non-small cell lung cancer before (chemo-) radiation
Článek Risk factor-based subphenotyping of heart failure in the community
Článek Higher underestimation of tumour size post-neoadjuvant chemotherapy with breast magnetic resonance imaging (MRI)—A concordance comparison cohort analysis
Článek Wealth accumulation in rotation forestry – Failure of the net present value optimization?
Článek Increased incidence of age-related macular degeneration in sensorineural hearing loss: A population-based cohort study
Článek Effects of a hot ambient operating theatre on manual dexterity, psychological and physiological parameters in staff during a simulated burn surgery
Článek Introduction of quality management in a National Reference Laboratory in Germany
Článek Common variants in glyoxalase I do not increase chronic pancreatitis risk
Článek Cognitive impairment in multiple sclerosis: An exploratory analysis of environmental and lifestyle risk factors
Článek Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop
Článek Development of an integrated fingerstick blood self-collection device for radiation countermeasures
Článek Concordance between gene expression in peripheral whole blood and colonic tissue in children with inflammatory bowel disease
Článek Effects of arthroscopic vs. mini-open rotator cuff repair on function, pain & range of motion. A systematic review and meta-analysis
Článek Engagement in care among women and their infants lost to follow-up under Option B+ in eSwatini
Článek The black sheep effect: The case of the deviant ingroup robot
Článek Implementation of an international standardized set of outcome indicators in pregnancy and childbirth in Kenya: Utilizing mobile technology to collect patient-reported outcomes
Článek The dynamics of Early Celtic consumption practices: A case study of the pottery from the Heuneburg
Článek Assessing hand dysfunction in cervical spondylotic myelopathy
Článek Variation in activity rates may explain sex-specific dorsal color patterns in Habronattus jumping spiders
Článek Acute sprint exercise transcriptome in human skeletal muscle
Článek Multiplex communities and the emergence of international conflict
Článek The impact of socioeconomic status on emergency department outcome in a low-income country setting: A registry-based analysis
Článek A cluster-randomized controlled trial of the effectiveness of the JUMP Math program of math instruction for improving elementary math achievement
Článek Transgenic mouse model for conditional expression of influenza hemagglutinin-tagged human SLC20A1/PIT1
Článek Fundraising and vote distribution: A non-equilibrium statistical approach
Článek Alteration of adipose tissue immune cell milieu towards the suppression of inflammation in high fat diet fed mice by flaxseed oil supplementation
Článek P16 methylation increases the sensitivity of cancer cells to the CDK4/6 inhibitor palbociclib
Článek Genetic mapping of distal femoral, stifle, and tibial radiographic morphology in dogs with cranial cruciate ligament disease
Článek Consumer beliefs about healthy foods and diets
Článek Is recent Afghanistan survey data suitable for fertility analysis? A regional investigation based on fertility inhibiting determinants
Článek Comparison of two swept-source optical coherence tomography biometers and a partial coherence interferometer
Článek Removal of anthracycline cytostatics from aquatic environment: Comparison of nanocrystalline titanium dioxide and decontamination agents
Článek A social media intervention to improve nutrition knowledge and behaviors of low income, pregnant adolescents and adult women
Článek Does self-reported pregnancy loss identify women at risk of an adverse cardiovascular phenotype in later life? Insights from UK Biobank
Článek Decision-based models of the implementation of interventions in systems of healthcare: Implementation outcomes and intervention effectiveness in complex service environments
Článek Dual number-based variational data assimilation: Constructing exact tangent linear and adjoint code from nonlinear model evaluations
Článek Use of IoT sensing and occupant surveys for determining the resilience of buildings to forest fire generated PM2.5
Článek Attenuation correction using 3D deep convolutional neural network for brain 18F-FDG PET/MR: Comparison with Atlas, ZTE and CT based attenuation correction
Článek Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface
Článek What is the optimum time for initiation of early mobilization in mechanically ventilated patients? A network meta-analysis
Článek Cognitive aptitude, peers, and trajectories of marijuana use from adolescence through young adulthood
Článek Does task delegation to non-physician health professionals improve quality of diabetes care? Results of a scoping review
Článek The Univariate Flagging Algorithm (UFA): An interpretable approach for predictive modeling
Článek Profiles and trajectories of impaired social cognition in people with Prader-Willi syndrome
Článek Engaging in prosocial behavior explains how high self-control relates to more life satisfaction: Evidence from three Chinese samples
Článek Prevalence and associated factors of depression among Korean adolescents
Článek Lipid profile changings after switching from rilpivirine/tenofovir disoproxil fumarate/emtricitabine to rilpivirine/tenofovir alafenamide/emtricitabine: Different effects in patients with or without baseline hypercholesterolemia
Článek Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
Článek Integrating continuous differential evolution with discrete local search for meander line RFID antenna design
Článek The impact of the French soda tax on prices and purchases. An ex post evaluation
Článek Potential biomarker identification for Friedreich’s ataxia using overlapping gene expression patterns in patient cells and mouse dorsal root ganglion
Článek Chile (Capsicum annuum) plants transformed with the RB gene from Solanum bulbocastanum are resistant to Phytophthora capsici
Článek Outcomes of acute coronary syndrome patients with concurrent extra-cardiac vascular disease in the era of transradial coronary intervention: A retrospective multicenter cohort study
Článek Myocardial function in patients with anomalous left coronary artery from the pulmonary artery syndrome: A long-term speckle tracking echocardiographic study
Článek Tidal inlet seafloor changes induced by recently built hard structures
Článek Extracellular matrix turnover and inflammation in chemically-induced TMJ arthritis mouse models
Článek Separating the effects of temperature and carbon allocation on the diel pattern of soil respiration in the different phenological stages in dry grasslands
Článek Sharing the filmic experience - The physiology of socio-emotional processes in the cinema
Článek A super-SILAC based proteomics analysis of diffuse large B-cell lymphoma-NOS patient samples to identify new proteins that discriminate GCB and non-GCB lymphomas
Článek Tissue-specific expression of IgG receptors by human macrophages ex vivo
Článek Clustering of the structures by using “snakes-&-dragons” approach, or correlation matrix as a signal
Článek Asymmetric valuation of gains and losses in effort-based decision making
Článek Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis
Článek Cell fishing: A similarity based approach and machine learning strategy for multiple cell lines-compound sensitivity prediction
Článek Machine learning prediction of emesis and gastrointestinal state in ferrets
Článek Geospatial correlates of early marriage and union formation in Ghana
Článek An alternative approach for estimating the number needed to treat for survival endpoints
Článek Impact of climate change on the integrity of the superstructure of deteriorated U.S. bridges
Článek Switching to bedaquiline for treatment of rifampicin-resistant tuberculosis in South Africa: A retrospective cohort analysis
Článek The impact of leishmaniasis on mental health and psychosocial well-being: A systematic review
Článek Evaluation of a screening algorithm using the Strengths and Difficulties Questionnaire to identify children with mental health problems: A five-year register-based follow-up on school performance and healthcare use
Článek Optimized fast GPU implementation of robust artificial-neural-networks for k-space interpolation (RAKI) reconstruction
Článek Refined distributed emotion vector representation for social media sentiment analysis
Článek Enhancing timeliness of drug overdose mortality surveillance: A machine learning approach
Článek Evaluation of clinical manifestations, health risks, and quality of life among women with polycystic ovary syndrome
Článek Agrobacterium tumefaciens-mediated transformation of a hevein-like gene into asparagus leads to stem wilt resistance
Článek Effect of a Mentor Mother Programme on retention of mother-baby pairs in HIV care: A secondary analysis of programme data in Uganda
Článek Iodine supplementation of lactating women and assessment of infant visual information processing and maternal and infant thyroid function: A randomized trial
Článek Within-subject variability in human retinal nerve fiber bundle width
Článek Comparison of conventional BLUP and single-step genomic BLUP evaluations for yearling weight and carcass traits in Hanwoo beef cattle using single trait and multi-trait models
Článek Do cognitive and non-cognitive abilities mediate the relationship between air pollution exposure and mental health?
Článek Molecular phylogeny and morphology reveal a new epiphytic species of Habenaria (Orchidaceae; Orchideae; Orchidinae) from Nepal
Článek Nitrogen nutrition is a key modulator of the sugar and organic acid content in citrus fruit
Článek Understanding variation in health service coverage and maternal health outcomes among districts in Rwanda – A qualitative study of local health workers’ perceptions
Článek Biased cognition in East Asian and Western cultures
Článek Robust policy evaluation from large-scale observational studies
Článek Delineation of high resolution climate regions over the Korean Peninsula using machine learning approaches
Článek Are spasticity, weakness, selectivity, and passive range of motion related to gait deviations in children with spastic cerebral palsy? A statistical parametric mapping study
Článek Conspicuous and cryptic reef fishes from a unique and economically important region in the northern Red Sea
Článek Evidence of ritual breakage of a ground stone tool at the Late Natufian site of Hilazon Tachtit cave (12,000 years ago)
Článek The occurrence of ‘Sleeping Beauty’ publications in medical research: Their scientific impact and technological relevance
Článek Assessing undergraduate student and faculty views on animal research: What do they know, whom do they trust, and how much do they care?
Článek A simplified vocal tract model for articulation of [s]: The effect of tongue tip elevation on [s]
Článek Anabolic steroids among resistance training practitioners
Článek New methods of removing debris and high-throughput counting of cyst nematode eggs extracted from field soil
Článek Vehicle configurations associated with anatomical-specific severe injuries resulting from traffic collisions
Článek Technology opportunity discovery by structuring user needs based on natural language processing and machine learning
Článek Design, synthesis, and structural elucidation of novel NmeNANAS inhibitors for the treatment of meningococcal infection
Článek Bringing fear into focus: The intersections of HIV and masculine gender norms in Côte d’Ivoire
Článek Scientific output scales with resources. A comparison of US and European universities
Článek Effect of host genotype and Eimeria acervulina infection on the metabolome of meat-type chickens
Článek Agrobacterium tumefaciens-mediated transformation and expression of GFP in Ascochyta lentis to characterize ascochyta blight disease progression in lentil
Článek Localization and dynamic change of saponins in Cyclocarya paliurus (Batal.) Iljinskaja
Článek Relatives’ attachment anxiety mediates the association between perceived loss and expressed emotion in early psychosis
Článek Beyond the metabolic syndrome: Visceral and marrow adipose tissues impair bone quantity and quality in Cushing’s disease
Článek Kinematic and electromyographic analysis of variations in Nordic hamstring exercise
Článek Trabeculectomy followed by phacoemulsification versus trabeculectomy alone: The Collaborative Bleb-Related Infection Incidence and Treatment Study
Článek Comparative characterization of bacterial communities in geese consuming of different proportions of ryegrass
Článek The intriguing effect of ethanol and nicotine on acetylcholine-sensitive potassium current IKAch: Insight from a quantitative model
Článek The accumulation of deficits approach to describe frailty
Článek Turing complete neural computation based on synaptic plasticity
Článek Specialized care improves outcomes for patients with cirrhosis who require general surgical operations
Článek 3D analysis of human islet amyloid polypeptide crystalline structures in Drosophila melanogaster
Článek Early detection and differential serodiagnosis of Mycoplasma hyorhinis and Mycoplasma hyosynoviae infections under experimental conditions
Článek Simultaneous cardiac and respiratory inhibition during seizure precedes death in the DBA/1 audiogenic mouse model of SUDEP
Článek Dopamine receptor antagonists effects on low-dimensional attractors of local field potentials in optogenetic mice
Článek Dinosaur ichnology and sedimentology of the Chignik Formation (Upper Cretaceous), Aniakchak National Monument, southwestern Alaska; Further insights on habitat preferences of high-latitude hadrosaurs
Článek Mechanical effects of MitraClip on leaflet stress and myocardial strain in functional mitral regurgitation – A finite element modeling study
Článek Seroprevalence of anti-Toxoplasma gondii antibodies in wild boars (Sus scrofa), hunting dogs, and hunters of Brazil
Článek Endovascular therapy of direct dural carotid cavernous fistulas – A therapy assessment study including long-term follow-up patient interviews
Článek An extinction event in planktonic Foraminifera preceded by stabilizing selection
Článek Optimal seismic retrofitting techniques for URM school buildings located in the southwestern Iberian peninsula
Článek The effect of a hiding box on stress levels and body weight in Dutch shelter cats; a randomized controlled trial
Článek Early versus late initiation of renal replacement therapy for acute kidney injury in critically ill patients: A systematic review and meta-analysis
Článek Deep brain activation patterns involved in virtual gait without and with a doorway: An fMRI study
Článek Fermented cereal-based Munkoyo beverage: Processing practices, microbial diversity and aroma compounds
Článek Measurement invariance tests of revisions to archaically worded items in the Mach IV scale
Článek The effect of food anticipation on cognitive function: An eye tracking study
Článek A meta-analysis of crop response patterns to nitrogen limitation for improved model representation
Článek Transforming growth factor beta 1 induces methylation changes in lung fibroblasts
Článek Hepatic disease and the risk of mortality of Vibrio vulnificus necrotizing skin and soft tissue infections: A systematic review and meta-analysis
Článek Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
Článek Management strategies for vaccinated animals after an outbreak of foot-and-mouth disease and the impact on return to trade
Článek RNA-Seq analysis reveals an essential role of tyrosine metabolism pathway in response to root-rot infection in Gerbera hybrida
Článek SpliceHetero: An information theoretic approach for measuring spliceomic intratumor heterogeneity from bulk tumor RNA-seq
Článek Large-scale phylogenomic analysis suggests three ancient superclades of the WUSCHEL-RELATED HOMEOBOX transcription factor family in plants
Článek Plasma sCD36 as non-circadian marker of chronic circadian disturbance in shift workers
Článek Trends in NBA and Euroleague basketball: Analysis and comparison of statistical data from 2000 to 2017
Článek In vivo human lower limb muscle architecture dataset obtained using diffusion tensor imaging
Článek Tobacco smoke exposure as a risk factor for human papillomavirus infections in women 18-26 years old in the United States
Článek The trigger-information-response model: Exploring health literacy during the first six months following a kidney transplantation
Článek The development of an evidence-based street food vending model within a socioecological framework: A guide for African countries
Článek Sociodemographic changes and trends in the rates of new perinatal HIV diagnoses and transmission in Spain from 1997 to 2015
Článek Patient navigation services for cancer care in low-and middle-income countries: A scoping review
Článek Weather extremes and perinatal mortality – Seasonal and ethnic differences in northern Sweden, 1800-1895
Článek Maternal and perinatal death surveillance and response in Ethiopia: Achievements, challenges and prospects
Článek Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis
Článek Extensive culturomics of 8 healthy samples enhances metagenomics efficiency
Článek Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen
Článek Quantitation of eumelanin and pheomelanin markers in diverse biological samples by HPLC-UV-MS following solid-phase extraction
Článek Kinetics of mean platelet volume predicts mortality in patients with septic shock
Článek An integrated epigenome and transcriptome analysis identifies PAX2 as a master regulator of drug resistance in high grade pancreatic ductal adenocarcinoma
Článek Combining CDK4/6 inhibitors ribociclib and palbociclib with cytotoxic agents does not enhance cytotoxicity
Článek Association of Medicaid expansion with health insurance coverage by marital status and sex
Článek Are current approaches for measuring access to clean water and sanitation inclusive of people with disabilities? Comparison of individual- and household-level access between people with and without disabilities in the Tanahun district of Nepal
Článek Monitoring Universal Health Coverage (UHC) in high Tuberculosis burden countries: Tuberculosis mortality an important tracer of UHC service coverage
Článek RCT evaluation of Skhokho: A holistic school intervention to prevent gender-based violence among South African Grade 8s
Článek On the use of Action Units and fuzzy explanatory models for facial expression recognition
Článek A systematic review of complementary and alternative medicine in oncology: Psychological and physical effects of manipulative and body-based practices
Článek What factors best explain attitudes to snow leopards in the Nepal Himalayas?
Článek Determinants of temperature sensitivity of soil respiration with the decline of a foundation species
Článek Mindfulness meditation, time judgment and time experience: Importance of the time scale considered (seconds or minutes)
Článek A survey of public attitudes toward uterus transplantation, surrogacy, and adoption in Japan
Článek Accurate dried blood spots collection in the community using non-medically trained personnel could support scaling up routine viral load testing in resource limited settings
Článek Genetic analysis of hsCRP in American Indians: The Strong Heart Family Study
Článek Antimicrobial efficacy of commercially available endodontic bioceramic root canal sealers: A systematic review
Článek The contribution of linear perspective cues and texture gradients in the perceptual rescaling of stimuli inside a Ponzo illusion corridor
Článek Cerebral blood flow imbalance is associated with motor outcome after pediatric arterial ischemic stroke
Článek Implementing advance care planning in routine nursing home care: The development of the theory-based ACP+ program
Článek Identifying genetic lineages through shape: An example in a cosmopolitan marine turtle species using geometric morphometrics
Článek The causality of borrowing: Lexical loans in Eurasian languages
Článek The uptake of the pharmacy-dispensed naloxone kit program in Ontario: A population-based study
Článek Gaze and body cues interplay during interactive requests
Článek Acetylcholine-mediated top-down attention improves the response to bottom-up inputs by deformation of the attractor landscape
Článek Deep architectures for long-term stock price prediction with a heuristic-based strategy for trading simulations
Článek Organizational justice and illness reporting among Japanese employees with chronic diseases
Článek CavBench: A benchmark for protein cavity detection methods
Článek Prevalence of methicillin-resistant Staphylococcus aureus in healthy Chinese population: A system review and meta-analysis
Článek Risk of pneumonia among residents living near goat and poultry farms during 2014-2016
Článek Lipid levels after childbirth and association with number of children: A population-based cohort study
Článek Birth outcomes in women who have taken adalimumab in pregnancy: A prospective cohort study
Článek ECMO with vasopressor use during early endotoxic shock: Can it improve circulatory support and regional microcirculatory blood flow?
Článek Classifying recurrent Mycobacterium tuberculosis cases in Georgia using MIRU-VNTR typing
Článek An observational study comparing HPV prevalence and type distribution between HPV-vaccinated and -unvaccinated girls after introduction of school-based HPV vaccination in Norway
Článek Sarcopenia as a prognostic factor for survival in patients with locally advanced gastroesophageal adenocarcinoma
Článek Attending to design when developing complex health interventions: A qualitative interview study with intervention developers and associated stakeholders
Článek Increased fecundity of Aphis fabae on Vicia faba plants following seed or leaf inoculation with the entomopathogenic fungus Beauveria bassiana
Článek Characterization of sound scattering layers in the Bay of Biscay using broadband acoustics, nets and video
Článek Conducting human challenge studies in LMICs: A survey of researchers and ethics committee members in Thailand
Článek Clinical use of SAND battery to evaluate language in patients with Progressive Supranuclear Palsy
Článek Robust automated reading of the skin prick test via 3D imaging and parametric surface fitting
Článek A psychoacoustic application for the adjustment of electrical hearing thresholds in cochlear implant patients
Článek The impact of shared knowledge on speakers’ prosody
Článek Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs
Článek Access to unauthorized hepatitis C generics: Perception and knowledge of physicians, pharmacists, patients and non-healthcare professionals
Článek Urban commuting dynamics in response to public transit upgrades: A big data approach
Článek Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
Článek When resolution does matter: Modelling indirect contacts in dairy farms at different levels of detail
Článek Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States
Článek Liquorice ingestion attenuates vasodilatation via exogenous nitric oxide donor but not via β2-adrenoceptor stimulation
Článek Adolescents living with HIV are at higher risk of death and loss to follow up from care: Analysis of cohort data from eight health facilities in Ethiopia
Článek Identification of social relation within pedestrian dyads
Článek Blood donation and health status based on SF-36: The mediating effect of cognition in blood donation
Článek Analysing linear multivariate pattern transformations in neuroimaging data
Článek Ecological aesthetic assessment of a rebuilt wetland restored from farmland and management implications for National Wetland Parks
Článek A method for predicting background advertisement exposure parameters in sporting events: Televised football game approach
Článek Self-reported and experimentally induced self-disgust is heightened in Parkinson’s disease: Contribution of behavioural symptoms
Článek Associations between nocturnal sleep duration, midday nap duration and body composition among adults in Southwest China
Článek Molecular detection and genetic characterization of Bartonella species from rodents and their associated ectoparasites from northern Tanzania
Článek Estimation of the number of HCV-positive patients in Italy
Článek Comparative cost analysis of point-of-care versus laboratory-based testing to initiate and monitor HIV treatment in South Africa
Článek QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
Článek Establishing an infrastructure for collaboration in primate cognition research
Článek Modeling environmental variability and network formation among pastoral nomadic households: Implications for the rise of the Mongol Empire
Článek Changes in real-life practice for hepatocellular carcinoma patients in the Republic of Korea over a 12-year period: A nationwide random sample study
Článek Illness beliefs and emotional responses in mildly disabled stroke survivors: A qualitative study
Článek Modeling the natural history of fatty liver using lifestyle–related risk factors: Effects of body mass index (BMI) on the life–course of fatty liver
Článek Comparative transcriptome analysis of galls from four different host plants suggests the molecular mechanism of gall development
Článek Experiences with peer support for breastfeeding in Beirut, Lebanon: A qualitative study
Článek Elucidating optical field directed hierarchical self-assembly of homogenous versus heterogeneous nanoclusters with femtosecond optical tweezers
Článek Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer
Článek Elevated serum alpha-1 antitrypsin is a major component of GlycA-associated risk for future morbidity and mortality
Článek Quality of labor and birth care in Sindh Province, Pakistan: Findings from direct observations at health facilities
Článek A comparison of sLASER and MEGA-sLASER using simultaneous interleaved acquisition for measuring GABA in the human brain at 7T
Článek Urinary Activin A is a novel biomarker reflecting renal inflammation and tubular damage in ANCA-associated vasculitis
Článek Pollock avoided hydrodynamic instabilities to paint with his dripping technique
Článek Metabolomic analysis of the occurrence of bitter fruits on grafted oriental melon plants
Článek Photo-degradation dynamics of five neonicotinoids: Bamboo vinegar as a synergistic agent for improved functional duration
Článek Use of acoustic emission to identify novel candidate biomarkers for knee osteoarthritis (OA)
Článek Cetacean biodiversity, spatial and temporal trends based on stranding records (1920-2016), Victoria, Australia
Článek Neutrophil elastase inhibitor purification strategy from cowpea seeds
Článek Coherent poly propagation materials with 3-dimensional photonic control over visible light
Článek Characterization of Worldwide Olive Germplasm Banks of Marrakech (Morocco) and Córdoba (Spain): Towards management and use of olive germplasm in breeding programs
Článek Can non-pharmacological interventions reduce hospital admissions in people with dementia? A systematic review
Článek Metarhizium robertsii ammonium permeases (MepC and Mep2) contribute to rhizoplane colonization and modulates the transfer of insect derived nitrogen to plants
Článek Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis
Článek Financial inclusion and intimate partner violence: What does the evidence suggest?
Článek Are intestinal helminths playing a positive role in tuberculosis risk? A systematic review and meta-analysis
Článek Money doesn’t matter! Householders’ intentions to reduce standby power are unaffected by personalised pecuniary feedback
Článek Validating a scale to measure engineers’ perceived self-efficacy for engineering education outreach
Článek Effect of d-cycloserine on fear extinction training in adults with social anxiety disorder
Článek Nursing students and nurses’ knowledge and attitudes regarding children’s pain: A comparative cross-sectional study
Článek Knowledge, attitudes and medical practice regarding hepatitis B prevention and management among healthcare workers in Northern Vietnam
Článek Development and validation of a symptom assessment tool for postmicturition dribble: A prospective, multicenter, observational study in Korea
Článek Identification of closely related Ixodes species by protein profiling with MALDI-TOF mass spectrometry
Článek When calculators lie: A demonstration of uncritical calculator usage among college students and factors that improve performance
Článek Socio-economic characteristics and career intentions of the WiSDOM health professional cohort in South Africa
Článek Social proximity in dairy calves is affected by differences in pessimism
Článek Amphibian skin-associated Pigmentiphaga: Genome sequence and occurrence across geography and hosts
Článek Expanded consumer niche widths may signal an early response to spatial protection
Článek Moral grandstanding in public discourse: Status-seeking motives as a potential explanatory mechanism in predicting conflict
Článek Adipose stem cells in reparative goat mastitis mammary gland
Článek Seventy-five mosses and liverworts found frozen with the late Neolithic Tyrolean Iceman: Origins, taphonomy and the Iceman’s last journey
Článek Hyaluronic acid and epidermal growth factor improved the bovine embryo quality by regulating the DNA methylation and expression patterns of the focal adhesion pathway
Článek Associations among knowledge, attitudes, and practices toward palliative care consultation service in healthcare staffs: A cross-sectional study
Článek A novel, wearable, electronic visual aid to assist those with reduced peripheral vision
Článek Celecoxib enhances the sensitivity of non-small-cell lung cancer cells to radiation-induced apoptosis through downregulation of the Akt/mTOR signaling pathway and COX-2 expression
Článek Mobile health-based physical activity intervention for individuals with spinal cord injury in the community: A pilot study
Článek Factors influencing cultivated ginseng (Panax ginseng C. A. Meyer) bioactive compounds
Článek Estimation of soil pH with geochemical indices in forest soils
Článek Multiplexed editing of a begomovirus genome restricts escape mutant formation and disease development
Článek Correlates of longitudinal leukocyte telomere length in the Costa Rican Longevity Study of Healthy Aging (CRELES): On the importance of DNA collection and storage procedures
Článek Collaboration and followership: A stochastic model for activities in social networks
Článek Evaluating access to oral anti-diabetic medicines: A cross-sectional survey of prices, availability and affordability in Shaanxi Province, Western China
Článek Comparison of corneal elevation and pachymetry measurements made by two state of the art corneal tomographers with different measurement principles
Článek Use of a pressure-sensing walkway system for biometric assessment of gait characteristics in goats
Článek Current status and direct medical cost of amyotrophic lateral sclerosis in the region of Catalonia: A population-based analysis
Článek Engineering a single-chain antibody against Trypanosoma cruzi metacyclic trypomastigotes to block cell invasion
Článek Gene editing of PKLR gene in human hematopoietic progenitors through 5’ and 3’ UTR modified TALEN mRNA
Článek Pelvic alignment changes during the perinatal period
Článek Interatrial blocks prevalence and risk factors for human immunodeficiency virus-infected persons
Článek Inhibitory interaction networks among coevolved Streptomyces populations from prairie soils
Článek Exploiting contextual information to improve call prediction
Článek The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development
Článek Live-attenuated H1N1 influenza vaccine candidate displays potent efficacy in mice and ferrets
Článek Robust, real-time generic detector based on a multi-feature probabilistic method
Článek Maize leaves drought-responsive genes revealed by comparative transcriptome of two cultivars during the filling stage
Článek Xenon lamps used for fruit surface sterilization can increase the content of total flavonols in leaves of Lactuca sativa L. without any negative effect on net photosynthesis
Článek An alternative procedure to obtain the mortality rate with non-linear functions: Application to the case of the Spanish population
Článek Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis
Článek Longitudinal assessment of post-surgical physical activity in endometrial and ovarian cancer patients
Článek THINGS: A database of 1,854 object concepts and more than 26,000 naturalistic object images
Článek Evaluation of ranibizumab and aflibercept for the treatment of diabetic macular edema in daily clinical practice
Článek Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM)
Článek Correction: Effect of curcuminoids and curcumin derivate products on thioredoxin-glutathione reductase from Taenia crassiceps cysticerci. Evidence suggesting a curcumin oxidation product as a suitable inhibitor
Článek Correction: Predicting childhood obesity using electronic health records and publicly available data
Článek Correction: Estimating the value of tropical coastal wetland habitats to fisheries: Caveats and assumptions
Článek Correction: The effects of sympathetic activity induced by ice water on blood flow and brachial artery flow-mediated dilatation response in healthy volunteers
Článek Determinants of frailty development and progression using a multidimensional frailty index: Evidence from the English Longitudinal Study of Ageing
Článek Supporting informed clinical trial decisions: Results from a randomized controlled trial evaluating a digital decision support tool for those with intellectual disability
Článek BMAL1 associates with chromosome ends to control rhythms in TERRA and telomeric heterochromatin
Článek Antimicrobial resistance genotypes and phenotypes of Campylobacter jejuni isolated in Italy from humans, birds from wild and urban habitats, and poultry
Článek Hepatopulmonary syndrome has low prevalence of pulmonary vascular abnormalities on chest computed tomography
Článek Early and long-term outcomes of coronary artery bypass surgery with and without use of heart-lung machine and with special respect to renal function - A retrospective study
Článek Analysis of determinants for in vitro resistance to the small molecule deubiquitinase inhibitor b-AP15
Článek The FTO genetic variants are associated with dietary intake and body mass index amongst Emirati population
Článek Correction: Lentivirus-Mediated Knockdown of Astrocyte Elevated Gene-1 Inhibits Growth and Induces Apoptosis through MAPK Pathways in Human Retinoblastoma Cells
Článek Relationship between self-disclosure to first acquaintances and subjective well-being in people with schizophrenia spectrum disorders living in the community
Článek Remarkable cell recovery from cerebral ischemia in rats using an adaptive escalator-based rehabilitation mechanism
Článek Integrating environmental and neighborhood factors in MaxEnt modeling to predict species distributions: A case study of Aedes albopictus in southeastern Pennsylvania
Článek Post-overdose interventions triggered by calling 911: Centering the perspectives of people who use drugs (PWUDs)
Článek The prevalence of intimate partner violence among couples signing up for universally offered parent preparation
Článek Analysis of gear surface morphology based on gray level co-occurrence matrix and fractal dimension
Článek Abiotic, present-day and historical effects on species, functional and phylogenetic diversity in dry grasslands of different age
Článek Clonality analysis of pulmonary tumors by genome-wide copy number profiling
Článek A relictual troglomorphic harvestman discovered in a volcanic cave of western Argentina: Otilioleptes marcelae, new genus, new species, and Otilioleptidae, new family (Arachnida, Opiliones, Gonyleptoidea)
Článek HIV drug resistance in persons who inject drugs enrolled in an HIV prevention trial in Indonesia, Ukraine, and Vietnam: HPTN 074
Článek Dietary acid load significantly predicts 10-years survival in patients underwent coronary artery bypass grafting (CABG) surgery
Článek Regulation of nitrogen availability results in changes in grain protein content and grain storage subproteomes in barley (Hordeum vulgare L.)
Článek Diagnostic test evaluation methodology: A systematic review of methods employed to evaluate diagnostic tests in the absence of gold standard – An update
Článek Longitudinal trends of and factors associated with inappropriate antibiotic prescribing for non-bacterial acute respiratory tract infection in Japan: A retrospective claims database study, 2012–2017
Článek Revealing users’ experience and social interaction outcomes following a web-based smoking prevention intervention for adolescents: A qualitative study
Článek Coordination of human movements resulting in motor strategies exploited by skilled players during a throwing task
Článek Impact of pericardiectomy on exercise capacity and sleep of patients with chronic constrictive pericarditis
Článek Blue-violet light decreases VEGFa production in an in vitro model of AMD
Článek Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
Článek Speed of processing training and depression in assisted and independent living: A randomized controlled trial
Článek Induced cardiomyocyte maturation: Cardiac transcription factors are necessary but not sufficient
Článek What happens in the brain of meditators when perception changes but not the stimulus?
Článek Comparison of HIV-1 Vif and Vpu accessory proteins for delivery of polyepitope constructs harboring Nef, Gp160 and P24 using various cell penetrating peptides
Článek Are degree of urbanisation and travel times to healthcare services associated with the processes of care and outcomes of heart failure? A retrospective cohort study based on administrative data
Článek A PDGFRβ-PI3K signaling axis mediates periosteal cell activation during fracture healing
Článek Influence of transcutaneous vagus nerve stimulation on cardiac vagal activity: Not different from sham stimulation and no effect of stimulation intensity
Článek Co-prescription network reveals social dynamics of opioid doctor shopping
Článek Contractile properties of superficial skeletal muscle affect postural control in healthy young adults: A test of the rambling and trembling hypothesis
Článek Correction: Measuring compassionate healthcare with the 12-item Schwartz Center Compassionate Care Scale
Článek Facilitators and barriers to traditional medicine use among cancer patients in Malawi
Článek Expression of CMV protein pp65 in cutaneous malignant melanoma
Článek The evolution of mental health outcomes across a combat deployment cycle: A longitudinal study of a Guam-based National Guard unit
Článek Caesarean section delivery and childhood obesity in a British longitudinal cohort study
Článek Incidence, characteristics, and mortality of infective endocarditis in France in 2011
Článek Pfcyp51 exclusively determines reduced sensitivity to 14α-demethylase inhibitor fungicides in the banana black Sigatoka pathogen Pseudocercospora fijiensis
Článek Evaluation of the bacterial ocular surface microbiome in clinically normal cats before and after treatment with topical erythromycin
Článek Histological and developmental insights into the herbivorous dentition of tapinocephalid therapsids
Článek Differential immunomodulation of T-cells by immunoglobulin replacement therapy in primary and secondary antibody deficiency
Článek A longitudinal study on perceived health in cardiovascular patients: The role of conscientiousness, subjective wellbeing and cardiac self-efficacy
Článek The effectiveness of interferon beta versus glatiramer acetate and natalizumab versus fingolimod in a Polish real-world population
Článek Honey bee and native solitary bee foraging behavior in a crop with dimorphic parental lines
Článek A reconnaissance survey of farmers’ awareness of hypomagnesaemic tetany in UK cattle and sheep farms
Článek Health workers’ perception of malaria rapid diagnostic test and factors influencing compliance with test results in Ebonyi state, Nigeria
Článek Conformational flexibility of fork-remodeling helicase Rad5 shown by full-ensemble hybrid methods
Článek The increasing importance of fellowships and career development awards in the careers of early-stage biomedical academic researchers
Článek Occurrence and stability of hetero-hexamer associations formed by β-carboxysome CcmK shell components
Článek Auxin driven indoleamine biosynthesis and the role of tryptophan as an inductive signal in Hypericum perforatum (L.)
Článek Dynamic activation of Wnt, Fgf, and Hh signaling during soft palate development
Článek Distance to large rivers affects fish diversity patterns in highly dynamic streams of Central Amazonia
Článek Real vs. immersive-virtual emotional experience: Analysis of psycho-physiological patterns in a free exploration of an art museum
Článek Modulation of brain tumor risk by genetic SNPs in PARP1gene: Hospital based case control study
Článek Processed meat intake and chronic disease morbidity and mortality: An overview of systematic reviews and meta-analyses
Článek Modeling ENSO impact on rice production in the Mekong River Delta
Článek The feasibility of a behavioral group intervention after weight-loss surgery: A randomized pilot trial
Článek EMR-integrated minimal core dataset for routine health care and multiple research settings: A case study for neuroinflammatory demyelinating diseases
Článek A review of a 13-month period of FilmArray Meningitis/Encephalitis panel implementation as a first-line diagnosis tool at a university hospital
Článek Increased BMI has a linear association with late-onset preeclampsia: A population-based study
Článek Autoantibody production significantly decreased with APRIL/BLyS blockade in murine chronic rejection kidney transplant model
Článek Telomere length in COPD: Relationships with physical activity, exercise capacity, and acute exacerbations
Článek Mutual role of ecto-5'-nucleotidase/CD73 and concentrative nucleoside transporter 3 in the intestinal uptake of dAMP
Článek Impact of faecal calprotectin measurement on clinical decision-making in patients with Crohn’s disease and ulcerative colitis
Článek Autophagy is important to the acidogenic metabolism of Aspergillus niger
Článek Continuous ratings of movie watching reveal idiosyncratic dynamics of aesthetic enjoyment
Článek Sensitivity and specificity of serum soluble interleukin-2 receptor for diagnosing sarcoidosis in a population of patients suspected of sarcoidosis
Článek Enhancing genomic selection by fitting large-effect SNPs as fixed effects and a genotype-by-environment effect using a maize BC1F3:4 population
Článek Quantitation and modeling of post-translational modifications in a therapeutic monoclonal antibody from single- and multiple-dose monkey pharmacokinetic studies using mass spectrometry
Článek Microbial induced solidification and stabilization of municipal solid waste incineration fly ash with high alkalinity and heavy metal toxicity
Článek Extracellular vesicles in human semen modulate antigen-presenting cell function and decrease downstream antiviral T cell responses
Článek A fault-tolerant aware scheduling method for fog-cloud environments
Článek Assessing the convergent validity between the automated emotion recognition software Noldus FaceReader 7 and Facial Action Coding System Scoring
Článek Deep learning based banana plant detection and counting using high-resolution red-green-blue (RGB) images collected from unmanned aerial vehicle (UAV)
Článek Comparison of cardiorespiratory fitness between preschool children with normal and excess body adipose ~ An observational study
Článek Intraregional differences in renal function in the Northern Netherlands: The Lifelines Cohort Study
Článek Effects of MCHM on yeast metabolism
Článek Signaling impacts of GMO labeling on fruit and vegetable demand
Článek Dietary specialization depending on ecological context and sexual differences in Asiatic black bears
Článek Electronic device use and beverage related sugar and caffeine intake in US adolescents
Článek Isolation and characterization of five novel probiotic strains from Korean infant and children faeces
Článek Endothelial nitric oxide synthase limits host immunity to control disseminated Candida albicans infections in mice
Článek Lack of detection of Candida nivariensis and Candida bracarensis among 440 clinical Candida glabrata sensu lato isolates in Kuwait
Článek Nonlinear dynamics captures brain states at different levels of consciousness in patients anesthetized with propofol
Článek Visual encounters on line transect surveys under-detect carnivore species: Implications for assessing distribution and conservation status
Článek Menstrual health communication among Indian adolescents: A mixed-methods study
Článek Fast fluorescence in situ hybridisation for the enhanced detection of MET in non-small cell lung cancer
Článek The influence of personality on the effect of iTBS after being stressed on cortisol secretion
Článek Parental and peer support and modelling in relation to domain-specific physical activity participation in boys and girls from Germany
Článek Oral delivery of water-soluble compounds to the phytoseiid mite Neoseiulus californicus (Acari: Phytoseiidae)
Článek Force perceptual bias caused by muscle activity in unimanual steering
Článek Providencia entomophila sp. nov., a new bacterial species associated with major olive pests in Tunisia
Článek Safety of intravitreal ziv-aflibercept in choroido-retinal vascular diseases: A randomised double-blind intervention study
Článek A novel framework for evaluating the impact of individual decision-making on public health outcomes and its potential application to study antiviral treatment collection during an influenza pandemic
Článek Reduction of the rocuronium-induced withdrawal reflex by MR13A10A, a generic rocuronium with a novel solution: A randomized, controlled study
Článek Emotionally expressed voices are retained in memory following a single exposure
Článek Megabenthic communities of the Ligurian deep continental shelf and shelf break (NW Mediterranean Sea)
Článek Election forensics: Using machine learning and synthetic data for possible election anomaly detection
Článek A randomized crossover trial comparing the Nifty cup to a medicine cup in preterm infants who have difficulty breastfeeding at Komfo Anokye Teaching Hospital (KATH) in Kumasi, Ghana
Článek The respiratory virome and exacerbations in patients with chronic obstructive pulmonary disease
Článek Genomic impact of severe population decline in a nomadic songbird
Článek Evolution of high-sensitivity troponin-T and echocardiography parameters in patients undergoing high efficiency on-line hemodiafiltration versus conventional low-flux hemodialysis
Článek Full genome characterization of 12 citrus tatter leaf virus isolates for the development of a detection assay
Článek Intake of myo-inositol hexaphosphate and urinary excretion of inositol phosphates in Wistar rats: Gavage vs. oral administration with sugar
Článek Enhancing financial security of female sex workers through a community-led intervention in India: Evidence from a longitudinal survey
Článek In-silico prediction of novel genes responsive to drought and salinity stress tolerance in bread wheat (Triticum aestivum)
Článek Genetic diversification of Panstrongylus geniculatus (Reduviidae: Triatominae) in northern South America
Článek Partitioning the risk of tuberculosis transmission in household contact studies
Článek Recommendation system in social networks with topical attention and probabilistic matrix factorization
Článek Using satellite-measured relative humidity for prediction of Metisa plana’s population in oil palm plantations: A comparative assessment of regression and artificial neural network models
Článek A drug interaction study investigating the effect of Rifabutin on the pharmacokinetics of Maraviroc in healthy subjects
Článek Emotional and behavioral problems, social competence and risk factors in 6–16-year-old students in Beijing, China
Článek Clinicopathologic and prognostic significance of tumor-associated macrophages in patients with hepatocellular carcinoma: A meta-analysis
Článek Involution of retinopathy of prematurity and neurodevelopmental outcomes after intravitreal bevacizumab treatment
Článek Travel demand and distance analysis for free-floating car sharing based on deep learning method
Článek Genome-wide identification, characterization, expression and enzyme activity analysis of coniferyl alcohol acetyltransferase genes involved in eugenol biosynthesis in Prunus mume
Článek Population analysis of D6-like plasmid prophage variants associated with specific IncC plasmid types in the emerging Salmonella Typhimurium ST213 genotype
Článek The high resource impact of reformatting requirements for scientific papers
Článek Egg yolk-free cryopreservation of bull semen
Článek Fine epitope mapping of glycoprotein Gn in Guertu virus
Článek Are hospital nurse staffing practices associated with postoperative cardiac events and death? A systematic review
Článek Dysregulation of macrophage development and phenotype in diabetic human macrophages can be rescued by Hoxa3 protein transduction
Článek Aging and the wandering brain: Age-related differences in the neural correlates of stimulus-independent thoughts
Článek Elucidating stygofaunal trophic web interactions via isotopic ecology
Článek Experimental characterization of two archaeal inosine 5'-monophosphate cyclohydrolases
Článek Scientist and data architect collaborate to curate and archive an inner ear electrophysiology data collection
Článek No effect of triple-pulse TMS medial to intraparietal sulcus on online correction for target perturbations during goal-directed hand and foot reaches
Článek Correction: Controlling Social Stress in Virtual Reality Environments
Článek TNF-α is responsible for the contribution of stromal cells to osteoclast and odontoclast formation during orthodontic tooth movement
Článek Viral respiratory infections and the oropharyngeal bacterial microbiota in acutely wheezing children
Článek Alteration of humoral, cellular and cytokine immune response to inactivated influenza vaccine in patients with Sickle Cell Disease
Článek To tweet or not to tweet, that is the question: A randomized trial of Twitter effects in medical education
Článek Visualizing a field of research: A methodology of systematic scientometric reviews
Článek True Colors: Commercially-acquired morphological genotypes reveal hidden allele variation among dog breeds, informing both trait ancestry and breed potential
Článek Impact of the detection of ζ-globin chains and hemoglobin Bart’s using immunochromatographic strip tests for α0-thalassemia (--SEA) differential diagnosis
Článek Cognitive impairment and medication adherence post-stroke: A five-year follow-up of the ASPIRE-S cohort
Článek Correction: Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana
Článek Correction: Precision public health: Mapping socioeconomic disparities in opioid dispensations at Swedish pharmacies by Multilevel Analysis of Individual Heterogeneity and Discriminatory Accuracy (MAIHDA)
Článek Epidemiological, clinical and laboratory characteristics of the measles resurgence in the Republic of Serbia in 2014-2015
Článek Macro-charcoal accumulation in floodplain wetlands: Problems and prospects for reconstruction of fire regimes and environmental conditions
Článek Stage at diagnosis and stage-specific survival of breast cancer in Latin America and the Caribbean: A systematic review and meta-analysis
Článek TEM8/ANTXR1-specific CAR T cells mediate toxicity in vivo
Článek Clinical impact of intraoperative hyperlactatemia during craniotomy
Článek Intubation with channeled versus non-channeled video laryngoscopes in simulated difficult airway by junior doctors in an out-of-hospital setting: A crossover manikin study
Článek Age and growth of stocked juvenile Shoal Bass in a tailwater: Environmental variation and accuracy of daily age estimates
Článek Sex-specific differences in risk factors of lymph node metastasis in patients with early gastric cancer
Článek The impact of electroconvulsive therapy on the spatial QRS-T angle and cardiac troponin T concentration in psychiatric patients
Článek BDNF, NT-3 and Trk receptor agonist monoclonal antibodies promote neuron survival, neurite extension, and synapse restoration in rat cochlea ex vivo models relevant for hidden hearing loss
Článek Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
Článek Comparison of cancer prevalence between patients with glomerulonephritis and the general population at the time of kidney biopsy
Článek Predictors of time to unfavorable treatment outcomes among patients with multidrug resistant tuberculosis in Oromia region, Ethiopia
Článek Experimentally validated simulation of coronary stents considering different dogboning ratios and asymmetric stent positioning
Článek The membrane effects of melittin on gastric and colorectal cancer
Článek Exclusive breastfeeding policy, practice and influences in South Africa, 1980 to 2018: A mixed-methods systematic review
Článek Hippocampal subfield volumes and pre-clinical Alzheimer’s disease in 408 cognitively normal adults born in 1946
Článek Uncorrected low hyperopia in young subjects induces postural instability even in those with clear visual acuity
Článek Membrane associated proteins of two Trichomonas gallinae clones vary with the virulence
Článek Awareness, current use of electronic cigarettes and associated smoking factors in Zhejiang Chinese adolescents
Článek Associations between pre-pregnancy BMI, gestational weight gain, and prenatal diet quality in a national sample
Článek Effects of continuous ketamine infusion on hemodynamics and mortality in critically ill children
Článek Optofluidic laser speckle image decorrelation analysis for the assessment of red blood cell storage
Článek Complete chloroplast genome sequence and phylogenetic analysis of Spathiphyllum 'Parrish'
Článek Strategic science communication as planned behavior: Understanding scientists’ willingness to choose specific tactics
Článek Understanding spatial patterns of soils for sustainable agriculture in northern Ethiopia’s tropical mountains
Článek Does Japan’s national nutrient-based dietary guideline improve lifestyle-related disease outcomes? A retrospective observational cross-sectional study
Článek Agitation near the end of life with dementia: An ethnographic study of care
Článek Tick-borne encephalitis virus infections in Germany. Seasonality and in-year patterns. A retrospective analysis from 2001-2018
Článek Encouraging perspective taking: Using narrative writing to induce empathy for others engaging in negative health behaviors
Článek Considerations of target surface area and the risk of radiosurgical toxicity
Článek Correction: A comparative study of RNA-Seq and microarray data analysis on the two examples of rectal-cancer patients and Burkitt Lymphoma cells
Článek Effect of using immobilization device in fluoroscopic study in pediatric patient: Focused on radiation dose reduction in voiding cystourethrogram
Článek Temporal trends in breast cancer survival by race and ethnicity: A population-based cohort study
Článek Contamination of hospital surfaces with respiratory pathogens in Bangladesh
Článek The impact on the bioenergetic status and oxidative-mediated tissue injury of a combined protocol of hypothermic and normothermic machine perfusion using an acellular haemoglobin-based oxygen carrier: The cold-to-warm machine perfusion of the liver
Článek Association between hospital interval and survival in patients with oral cancer: A waiting time paradox
Článek Translating a stress management intervention for rural Latina breast cancer survivors: The Nuevo Amanecer-II
Článek Comprehensive management of obstructive sleep apnea by telemedicine: Clinical improvement and cost-effectiveness of a Virtual Sleep Unit. A randomized controlled trial
Článek Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning
Článek Harnessing therapeutic viruses as a delivery vehicle for RNA-based therapy
Článek Korean soybean core collection: Genotypic and phenotypic diversity population structure and genome-wide association study
Článek Resistance profile of the HIV-1 maturation inhibitor GSK3532795 in vitro and in a clinical study
Článek Measurement of structural integrity of the spinal cord in patients with amyotrophic lateral sclerosis using diffusion tensor magnetic resonance imaging
Článek Typological analysis of public-private partnerships in the veterinary domain
Článek Improved clinical outcome after medial open-wedge osteotomy despite cartilage lesions in the lateral compartment
Článek Rapid and structure-specific cellular uptake of selected steroids
Článek Avoiding gambling harm: An evidence-based set of safe gambling practices for consumers
Článek Epidemiology of chronic pain in Ukraine: Findings from the World Mental Health Survey
Článek PIAS1 is not suitable as a urothelial carcinoma biomarker protein and pharmacological target
Článek Double triage to identify poorly annotated genes in maize: The missing link in community curation
Článek N-acetyl cysteine restores the fertility of vitrified–warmed mouse oocytes derived through ultrasuperovulation
Článek Architecture of population-differentiated polymorphisms in the human genome
Článek Linguistic neutrosophic power Muirhead mean operators for safety evaluation of mines
Článek Extracellular vesicles nanoarray technology: Immobilization of individual extracellular vesicles on nanopatterned polyethylene glycol-lipid conjugate brushes
Článek Exercise intensity-dependent effects of arm and leg-cycling on cognitive performance
Článek How information about perpetrators’ nature and nurture influences assessments of their character, mental states, and deserved punishment
Článek Stringent response governs the oxidative stress resistance and virulence of Francisella tularensis
Článek In silico assessment of human Calprotectin subunits (S100A8/A9) in presence of sodium and calcium ions using Molecular Dynamics simulation approach
Článek Sustained activity of novel THIOMAB antibody-antibiotic conjugate against Staphylococcus aureus in a mouse model: Longitudinal pharmacodynamic assessment by bioluminescence imaging
Článek Measurable residual disease monitoring for patients with acute myeloid leukemia following hematopoietic cell transplantation using error corrected hybrid capture next generation sequencing
Článek Characteristics of complementary medicine therapists in Switzerland: A cross-sectional study
Článek Lack of generalization between explicit and implicit visuomotor learning
Článek Diet modulates cecum bacterial diversity and physiological phenotypes across the BXD mouse genetic reference population
Článek Effects of temperature and salinity on respiratory losses and the ratio of photosynthesis to respiration in representative Antarctic phytoplankton species
Článek Self-reported non-receipt of HIV test results: A silent barrier to HIV epidemic control in Mozambique
Článek Plasma midkine concentrations in healthy children, children with increased and decreased adiposity, and children with short stature
Článek Echocardiography overestimates LV mass in the elderly as compared to cardiac CT
Článek Efficacy of cotrimoxazole (Sulfamethoxazole-Trimethoprim) as a salvage therapy for the treatment of bone and joint infections (BJIs)
Článek Molecular mechanism of antimicrobial activity of chlorhexidine against carbapenem-resistant Acinetobacter baumannii
Článek First identification of the benzimidazole resistance-associated F200Y SNP in the beta-tubulin gene in Ascaris lumbricoides
Článek Green synthesis of silver nanoparticles using Solanum mammosum L. (Solanaceae) fruit extract and their larvicidal activity against Aedes aegypti L. (Diptera: Culicidae)
Článek Enterovirus 71 seroepidemiology in Taiwan in 2017 and comparison of those rates in 1997, 1999 and 2007
Článek Effect of statin on life prognosis in Japanese patients undergoing hemodialysis
Článek 13C and 15N NMR identification of product compound classes from aqueous and solid phase photodegradation of 2,4,6-trinitrotoluene
Článek Off-target effects of CRISPRa on interleukin-6 expression
Článek Exploring the antimicrobial properties of dark-operating ceramic-based nanocomposite materials for the disinfection of indoor air
Článek Experienced males recognise and avoid mating with non-virgin females in the western flower thrips
Článek Liver transplantation in patients with a history of migration—A German single center comparative analysis
Článek Retraction: The Different Role of Notch1 and Notch2 in Astrocytic Gliomas
Článek Correction: Understanding climate change from a global analysis of city analogues
Článek Comparison of 4D Flow MRI to 2D Flow MRI in the pulmonary arteries in healthy volunteers and patients with pulmonary hypertension
Článek The relationship between sleep quality, inappropriate medication use and frailty among older adults in aged care homes in Malaysia
Článek Clonal diversity and spatial genetic structure in the long-lived herb, Prairie trillium
Článek Medication histories documentation at the community pharmacy setting: A study from Jordan
Článek Population dynamics of an invasive bird parasite, Philornis downsi (Diptera: Muscidae), in the Galapagos Islands
Článek Because you had a bad day: General and daily relations between reactive temperament, emotion regulation, and depressive symptoms in youth
Článek Socioeconomic status and alcohol use disorders across the lifespan: A co-relative control study
Článek Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase
Článek Polymorphisms in vasoactive eicosanoid genes of kidney donors affect biopsy scores and clinical outcomes in renal transplantation
Článek Adjuvant therapeutic potential of tonabersat in the standard treatment of glioblastoma: A preclinical F98 glioblastoma rat model study
Článek Validation and perception of a key feature problem examination in neurology
Článek Gender affirmative HIV care framework: Decisions on feminizing hormone therapy (FHT) and antiretroviral therapy (ART) among transgender women
Článek Quantifying international human mobility patterns using Facebook Network data
Článek Localization and characterization of thyroid microcalcifications: A histopathological study
Článek Young children are the main victims of fast food induced obesity in Brazil
Článek Understanding self-reported importance of religion/spirituality in a North American sample of individuals at risk for familial depression: A principal component analysis
Článek Standing balance of professional ballet dancers and non-dancers under different conditions
Článek 30-day unplanned readmission rate in otolaryngology patients: A population-based study in Thuringia, Germany
Článek Physical exercise stimulates salivary secretion of cystatins
Článek Vintage electronics for trusted radiation measurements and verified dismantlement of nuclear weapons
Článek Keepin’ it real: Linguistic models of authenticity judgments for artificially generated rap lyrics
Článek Carbon emission reduction and profit distribution mechanism of construction supply chain with fairness concern and cap-and-trade
Článek Modulations of microbehaviour by associative memory strength in Drosophila larvae
Článek Changes in peak expiratory flow rates using two head-tilt/chin-lift maneuver angles in young healthy conscious volunteers
Článek 3D image analysis reveals differences of CD30 positive cells and network formation in reactive and malignant human lymphoid tissue (classical Hodgkin Lymphoma)
Článek Change in larval fish assemblage in a USA east coast estuary estimated from twenty-six years of fixed weekly sampling
Článek 2-methylquinazoline derivative F7 as a potent and selective HDAC6 inhibitor protected against rhabdomyolysis-induced acute kidney injury
Článek Correction: Reducing growth and developmental problems in children: Development of an innovative postnatal risk assessment
Článek Comparative analysis of the vertebral pneumatization in pterosaurs (Reptilia: Pterosauria) and extant birds (Avialae: Neornithes)
Článek Associations between cognitive performance and sigma power during sleep in children with attention-deficit/hyperactivity disorder, healthy children, and healthy adults
Článek Retraction: MiR-375 Is Epigenetically Downregulated by HPV-16 E6 Mediated DNMT1 Upregulation and Modulates EMT of Cervical Cancer Cells by Suppressing lncRNA MALAT1
Článek Comparing growth patterns of three species: Similarities and differences
Článek Iron is increased in the brains of ageing mice lacking the neurofilament light gene
Článek Economic evaluation of community acquired pneumonia management strategies: A systematic review of literature
Článek A participatory community case study of periurban coastal flood vulnerability in southern Ecuador
Článek Chemotherapy-induced cellular senescence suppresses progression of Notch-driven T-ALL
Článek Mitochondrial dysfunctions in leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL)
Článek MRI-based visualization of rTMS-induced cortical plasticity in the primary motor cortex
Článek Knowledge, attitudes, and practices related to anthrax and animal care: A case-control study in Georgia
Článek Information diffusion in signed networks
Článek Nurses’ perspectives on their communication with patients in busy oncology wards: A qualitative study
Článek Inflammation in acute coronary syndrome: Expression of TLR2 mRNA is increased in platelets of patients with ACS
Článek ART is key to clearing oncogenic HPV genotypes (HR-HPV) in anal mucosa of HIV-positive MSM
Článek Retraction: Fluid–Structure Interaction-Based Biomechanical Perception Model for Tactile Sensing
Článek Correction: Role of Caveolin-1 in Atrial Fibrillation as an Anti-Fibrotic Signaling Molecule in Human Atrial Fibroblasts
Článek The French adaptation and validation of the Partners in Health (PIH) scale among patients with chronic conditions seen in primary care
Článek Depth inversion with a 3D structure influences brightness perception
Článek Analysis of group evolution prediction in complex networks
Článek Effect of aridity and dune type on rhizosphere soil bacterial communities of Caragana microphylla in desert regions of northern China
Článek Knowledge, attitudes, and practices related to TB among the general population of Ethiopia: Findings from a national cross-sectional survey
Článek Correction: Personality traits in companion dogs—Results from the VIDOPET
Článek MANET 3.0: Hierarchy and modularity in evolving metabolic networks
Článek Agreement study on gait assessment using a video-assisted rating method in patients with idiopathic normal-pressure hydrocephalus
Článek Single fixed low-dose rituximab as induction therapy suppresses de novo donor-specific anti-HLA antibody production in ABO compatible living kidney transplant recipients
Článek Myomesin is part of an integrity pathway that responds to sarcomere damage and disease
Článek Acute normobaric hypoxia does not affect the simultaneous exercise-induced increase in circulating BDNF and GDNF in young healthy men: A feasibility study
Článek Systematic investigation on quad-metallic AgAuPdPt and tri-metallic AuPdPt NPs through the solid-state dewetting of quad-layer Ag/Au/Pd/Pt thin films on c-plane sapphire
Článek Video lecturing in Clicker-assisted English flipped class
Článek Significant changes of the choroid in patients with ocular ischemic syndrome and symptomatic carotid artery stenosis
Článek Skewed T cell responses to Epstein-Barr virus in long-term asymptomatic kidney transplant recipients
Článek Candidate genes expression profiling during wilting in chickpea caused by Fusarium oxysporum f. sp. ciceris race 5
Článek Host plants influence the composition of the gut bacteria in Henosepilachna vigintioctopunctata
Článek ‘We are all serving the same Ugandans’: A nationwide mixed-methods evaluation of private sector surgical capacity in Uganda
Článek The association between flavor capsule cigarette use and sociodemographic variables: Evidence from Chile
Článek Relations between gross motor skills and executive functions, controlling for the role of information processing and lapses of attention in 8-10 year old children
Článek Linalool acts as a fast and reversible anesthetic in Hydra
Článek Double burden of maternal and child malnutrition and socioeconomic status in urban Sri Lanka
Článek Using isotemporal substitution to predict the effects of changing physical behaviour on older adults’ cardio-metabolic profiles
Článek Adherence to general medical checkup and cancer screening guidelines according to self-reported smoking status: Korea National Health and Nutrition Examination Survey (KNHANES) 2010–2012
Článek Do older English adults exhibit day-to-day compensation in sedentary time and in prolonged sedentary bouts? An EPIC-Norfolk cohort analysis
Článek Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue
Článek Correction: Factors associated with hospitalization and death among TB/HIV co-infected persons in Porto Alegre, Brazil
Článek An ex vivo tissue model of cartilage degradation suggests that cartilage state can be determined from secreted key protein patterns
Článek Reporting adverse events related to medical devices: A single center experience from a tertiary academic hospital
Článek Enterohemorrhagic Escherichia coli infection inhibits colonic thiamin pyrophosphate uptake via transcriptional mechanism
Článek Estimating biodiversity changes in the Camargue wetlands: An expert knowledge approach
Článek Decreased risk of Parkinson’s disease in diabetic patients with thiazolidinediones therapy: An exploratory meta-analysis
Článek Retraction: Carboxyamidotriazole-Orotate Inhibits the Growth of Imatinib-Resistant Chronic Myeloid Leukaemia Cells and Modulates Exosomes-Stimulated Angiogenesis
Článek Lipoarabinomannan from Mycobacterium indicus pranii shows immunostimulatory activity and induces autophagy in macrophages
Článek A measure of inconsistencies in intertemporal choice
Článek A novel radiopaque tissue marker for soft tissue localization and in vivo length and area measurements
Článek Anatomical, histological and computed tomography comparisons of the eye and adnexa of crab-eating fox (Cerdocyon thous) to domestic dogs
Článek Subpopulation augmentation among habitat patches as a tool to manage an endangered Mojave Desert wetlands-dependent rodent during anthropogenic restricted water climate regimes
Článek Characteristics of CD5-positive diffuse large B-cell lymphoma among Koreans: High incidence of BCL2 and MYC double-expressors
Článek Fossil tabulate corals reveal outcrops of Paleozoic sandstones in the Atlantic Coastal Plain Province, Southeastern USA
Článek Coming and going – Historical distributions of the European oyster Ostrea edulis Linnaeus, 1758 and the introduced slipper limpet Crepidula fornicata Linnaeus, 1758 in the North Sea
Článek Behavioral predictors of subsequent respiratory illness signs in dogs admitted to an animal shelter
Článek Novel chemotherapeutic agent, FND-4b, activates AMPK and inhibits colorectal cancer cell proliferation
Článek Can emotional intelligence be improved? A randomized experimental study of a business-oriented EI training program for senior managers
Článek Genetic variability in landraces populations and the risk to lose genetic variation. The example of landrace ‘Kyperounda’ and its implications for ex situ conservation
Článek Sleep problems are a strong predictor of stress-related metabolic changes in police officers. A prospective study
Článek Age, sex and primary care setting differences in patients’ perception of community healthcare seeking behaviour towards health services
Článek Kinematic analyses including finite helical axes of drop jump landings demonstrate decreased knee control long after anterior cruciate ligament injury
Článek Effects of model inaccuracies on reaching movements with intermittent control
Článek C-reactive protein to albumin ratio predicts the outcome in renal cell carcinoma: A meta-analysis
Článek A new synthetic lure for management of the invasive giant African snail, Lissachatina fulica
Článek Bridging solvent molecules mediate RNase A – Ligand binding
Článek Variation in methods, results and reporting in electronic health record-based studies evaluating routine care in gout: A systematic review
Článek The use of prophylactic intra-aortic balloon pump in high-risk patients undergoing coronary artery bypass grafting
Článek Compliance with clinical guidelines for breast cancer management: A population-based study of quality-of-care indicators in France
Článek Dysregulated T helper type 1 (Th1) and Th17 responses in elderly hospitalised patients with infection and sepsis
Článek Acute effects of differential learning on football kicking performance and in countermovement jump
Článek Escherichia coli and Salmonella spp. isolated from Australian meat chickens remain susceptible to critically important antimicrobial agents
Článek Play like me: Similarity in playfulness promotes social play
Článek Awareness of family health history in a predominantly young adult population
Článek Care pathways during a child’s final illness in rural South Africa: Findings from a social autopsy study
Článek Intranasal lidocaine for acute migraine: A meta-analysis of randomized controlled trials
Článek Evaluating case management as a complex intervention: Lessons for the future
Článek Allele-specific enhancer interaction at the Peg3 imprinted domain
Článek Has the “M” word been framed? Marijuana, cannabis, and public opinion
Článek Is spontaneous normalization of systolic blood pressure within 24 hours after ischemic stroke onset related with favorable outcomes?
Článek Mixotrophic cultivation of Spirulina platensis in dairy wastewater: Effects on the production of biomass, biochemical composition and antioxidant capacity
Článek Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis
Článek Stable carbon isotope diagnostics of mammalian metabolism, a high-resolution isotomics approach using amino acid carboxyl groups
Článek Factors associated with depressive symptoms in patients with ankylosing spondylitis in Northern Taiwan
Článek A multicenter prospective study of 515 febrile neutropenia episodes in Argentina during a 5-year period
Článek Retraction: Reversal Effect of ST6GAL 1 on Multidrug Resistance in Human Leukemia by Regulating the PI3K/Akt Pathway and the Expression of P-gp and MRP1
Článek Potential role for microRNA-16 (miR-16) and microRNA-93 (miR-93) in diagnosis and prediction of disease progression in mycosis fungoides in Egyptian patients
Článek Rapid visual categorization is not guided by early salience-based selection
Článek Relative contribution of climate and non-climate drivers in determining dynamic rates of boreal birds at the edge of their range
Článek A simple-to-use nomogram to predict long term survival of patients undergoing coronary artery bypass grafting (CABG) using bilateral internal thoracic artery grafting technique
Článek Effects of sonication parameters on transcranial focused ultrasound brain stimulation in an ovine model
Článek Antenatal and intrapartum interventions for reducing caesarean section, promoting vaginal birth, and reducing fear of childbirth: An overview of systematic reviews
Článek Relative species abundance successfully predicts nestedness and interaction frequency of monthly pollination networks in an alpine meadow
Článek Correction: RCER: Reliable Cluster-based Energy-aware Routing protocol for heterogeneous Wireless Sensor Networks
Článek Amphibians on the hotspot: Molecular biology and conservation in the South American Atlantic Rainforest
Článek Role of psychosocial status in predicting health-related quality of life at 1-year follow-up among newly diagnosed people living with HIV
Článek Application of national pollutant inventories for monitoring trends on dioxin emissions from stationary industrial sources in Australia, Canada and European Union
Článek The association between vaccination confidence, vaccination behavior, and willingness to recommend vaccines among Finnish healthcare workers
Článek Retraction: Interleukin-1β-Induced Autophagy-Related Gene 5 Regulates Proliferation of Embryonic Stem Cell-Derived Odontoblastic Cells
Článek A localized sanitation status index as a proxy for fecal contamination in urban Maputo, Mozambique
Článek E2 protein is the major determinant of specificity at the human papillomavirus origin of replication
Článek “You’re kind of at war with yourself as a nurse”: Perspectives of inpatient nurses on treating people who present with a comorbid opioid use disorder
Článek A refinement approach in a mouse model of rehabilitation research. Analgesia strategy, reduction approach and infrared thermography in spinal cord injury
Článek Circulating cell-free DNA from plasma undergoes less fragmentation during bisulfite treatment than genomic DNA due to low molecular weight
Článek Understanding fatigue in adults with visual impairment: A path analysis study of sociodemographic, psychological and health-related factors
Článek Retraction: Mouse-Induced Pluripotent Stem Cells Differentiate into Odontoblast-Like Cells with Induction of Altered Adhesive and Migratory Phenotype of Integrin
Článek A novel analytical method to assess the effect of imipenem/cilastatin on liver function laboratory indexes in Chinese underage inpatients: Probability distribution curve
Článek The cause of anorexia and proportion of its recovery in older adults without underlying disease: Results of a retrospective study
Článek Referral and admission to intensive care: A qualitative study of doctors’ practices in a Tanzanian university hospital
Článek Agreement between clinical and non-clinical digital manometer for assessing maximal respiratory pressures in healthy subjects
Článek Heads or tails? Differential translational regulation in cercarial heads and tails of schistosome worms
Článek Horizontal seed dispersal by dung beetles reduced seed and seedling clumping, but did not increase short-term seedling establishment
Článek The coupling of the M2 muscarinic receptor to its G protein is voltage dependent
Článek Subsidy strategy of pharmaceutical e-commerce platform based on two-sided market theory
Článek Shoulder girdle injuries involving the medial clavicle differ from lateral clavicle injuries with a focus on concomitant injuries and management strategies: A retrospective study based on nationwide routine data
Článek Unraveling middle childhood attachment-related behavior sequences using a micro-coding approach
Článek Divergence history and hydrothermal vent adaptation of decapod crustaceans: A mitogenomic perspective
Článek Duration of solid fuel cookstove use is associated with increased risk of acute lower respiratory infection among children under six months in rural central India
Článek Evolving flocking in embodied agents based on local and global application of Reynolds’ rules
Článek Non-invasive contrast enhanced ultrasound molecular imaging of inflammation in autoimmune myocarditis for prediction of left ventricular fibrosis and remodeling
Článek Care coordination for chronic and complex health conditions: An experienced based co-design study engaging consumer and clinician groups for service improvement
Článek Association between socioeconomic status and diet quality in Mexican men and women: A cross-sectional study
Článek Surveillance of panicle positions by unmanned aerial vehicle to reveal morphological features of rice
Článek Is Betula carpatica genetically distinctive? A morphometric, cytometric and molecular study of birches in the Bohemian Massif with a focus on Carpathian birch
Článek Correction: Sequential analysis of myocardial gene expression with phenotypic change: Use of cross-platform concordance to strengthen biologic relevance
Článek The genome of the migratory nematode, Radopholus similis, reveals signatures of close association to the sedentary cyst nematodes
Článek Factors associated with pneumococcal carriage and density in infants and young children in Laos PDR
Článek Overlap between telangiectasia and photoreceptor loss increases with progression of macular telangiectasia type 2
Článek The role of hope for health professionals in rehabilitation: A qualitative study on unfavorable prognosis communication
Článek Expression of miR-34a-5p is up-regulated in human colorectal cancer and correlates with survival and clock gene PER2 expression
Článek Molecular characterization and In Vitro synthesis of infectious RNA of a Turnip vein-clearing virus isolated from Alliaria petiolata in Hungary
Článek Assessment of client satisfaction with pharmacist services at outpatient pharmacy of Tikur Anbessa Specialized Hospital
Článek Thermogravimetric analysis of the co-combustion of coal and polyvinyl chloride
Článek Roles of leptin in the recovery of muscle and bone by reloading after mechanical unloading in high fat diet-fed obese mice
Článek Unexpected high seroprevalence of hepatitis E virus in patients with alcohol-related cirrhosis
Článek APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy
Článek Integrating a gait analysis test in hospital rehabilitation: A service design approach
Článek Correction: Bioactivity-guided isolation of rosmarinic acid as the principle bioactive compound from the butanol extract of Isodon rugosus against the pea aphid, Acyrthosiphon pisum
Článek Soil-borne fungi influence seed germination and mortality, with implications for coexistence of desert winter annual plants
Článek Applying Corrigan’s progressive model of self-stigma to people with depression
Článek Do psychosocial factors modify the negative association between disability and life satisfaction in old age?
Článek Public perception of coastal habitat loss and habitat creation using artificial floating islands in the UK
Článek Transcriptional foliar profile of the C3-CAM bromeliad Guzmania monostachia
Článek Application of tuned liquid column ball damper (TLCBD) for improved vibration control performance of multi-storey structure
Článek Do protection gradients explain patterns in herbivore densities? An example with ungulates in Zambia’s Luangwa Valley
Článek Consequences of chronic bacterial infection in Drosophila melanogaster
Článek The effects of environmental parameters on the microbial activity in peat-bog lakes
Článek Differences in protein structural regions that impact functional specificity in GT2 family β-glucan synthases
Článek Correction: Identification of lysine methylation in the core GTPase domain by GoMADScan
Článek Changes of absorbed dose rate in air in metropolitan Tokyo relating to radiocesium released from the Fukushima Daiichi Nuclear Power Plant accident: Results of a five-year study
Článek Histological chorioamnionitis, antenatal steroids, and neonatal outcomes in very low birth weight infants: A nationwide study
Článek A machine learning approach for the prediction of pulmonary hypertension
Článek Altered microbiota, fecal lactate, and fecal bile acids in dogs with gastrointestinal disease
Článek Impaired processing of threat in psychopathy: A systematic review and meta-analysis of factorial data in male offender populations
Článek Dimethyl disulfide (DMDS) as an effective soil fumigant against nematodes in China
Článek Risk factors for hepatitis C infection among adult patients in Kedah state, Malaysia: A case–control study
Článek Factoring a 2 x 2 contingency table
Článek The development of connectives in three to five-year-old monolingual Spanish-speaking children
Článek Foliar plasticity related to gradients of heat and drought stress across crown orientations in three Mediterranean Quercus species
Článek The evolution of haploid chromosome numbers in Meliponini
Článek Bloodstream infections caused by Escherichia coli in onco-haematological patients: Risk factors and mortality in an Italian prospective survey
Článek Facial features and unethical behavior – Doped athletes show higher facial width-to-height ratios than non-doping sanctioned athletes
Článek Non-resolution of non-alcoholic fatty liver disease (NAFLD) among urban, adult Sri Lankans in the general population: A prospective, cohort follow-up study
Článek Left ventricular simulation of cardiac compression: Hemodynamics and regional mechanics
Článek Are we ready for scaling up restoration actions? An insight from Mediterranean macroalgal canopies
Článek Age-related changes in size, bone microarchitecture and volumetric bone mineral density of the mandible in the harbor seal (Phoca vitulina)
Článek Parasitism modifies the direct effects of warming on a hemiparasite and its host
Článek Analytic performance of PANArray HPV and HPV 9G DNA chip tests for genotyping of high-risk human papillomavirus in cervical ThinPrep PreservCyt samples
Článek Transcript profile of skeletal muscle lipid metabolism genes affected by diet in a piglet model of low birth weight
Článek Differential impact of smoking on cardiac or non-cardiac death according to age
Článek Influence of injectate volume on paravertebral spread in erector spinae plane block: An endoscopic and anatomical evaluation
Článek Correction: Nitrogen starvation induces distinct photosynthetic responses and recovery dynamics in diatoms and prasinophytes
Článek Healthcare needs and programmatic gaps in transition from pediatric to adult care of vertically transmitted HIV infected adolescents in India
Článek Retraction: Neuronal Differentiation Dictates Estrogen-Dependent Survival and ERK1/2 Kinetic by Means of Caveolin-1
Článek Correction: Disability and its association with sociodemographic factors among elderly persons residing in an urban resettlement colony, New Delhi, India
Článek Antibacterial activity of varying UMF-graded Manuka honeys
Článek Correction: Synthesis of IL-6 by Hepatocytes Is a Normal Response to Common Hepatic Stimuli
Článek Detection and genetic characterization of Echinococcus granulosus mitochondrial DNA in serum and formalin-fixed paraffin embedded cyst tissue samples of cystic echinococcosis patients
Článek Deep-learning-based risk stratification for mortality of patients with acute myocardial infarction
Článek Links between meaning in life and physical quality of life after rehabilitation: Mediating effects of positive experiences with physical exercises and mobility
Článek The estrogen effect; clinical and histopathological evidence of dichotomous influences in dogs with spontaneous mammary carcinomas
Článek The association between socioeconomic status, psychopathological symptom burden in mothers, and early childhood caries of their children
Článek Increased rate of FEV1 decline in HIV patients despite effective treatment with HAART
Článek Parallels between stream and coastal water quality associated with groundwater discharge
Článek Determinants of anemia among pregnant women attending antenatal care in Horo Guduru Wollega Zone, West Ethiopia: Unmatched case-control study
Článek Depression and anxiety among pregnant women living with HIV in Kilimanjaro region, Tanzania
Článek Shift in trophic niches of soil microarthropods with conversion of tropical rainforest into plantations as indicated by stable isotopes (15N, 13C)
Článek On the development of sleep states in the first weeks of life
Článek CCR5 receptor antagonism inhibits hepatitis C virus (HCV) replication in vitro
Článek Investigation into pathophysiology of naturally occurring palatal instability and intermittent dorsal displacement of the soft palate (DDSP) in racehorses: Thyro-hyoid muscles fatigue during exercise
Článek Correction: Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts
Článek MitoToxy assay: A novel cell-based method for the assessment of metabolic toxicity in a multiwell plate format using a lactate FRET nanosensor, Laconic
Článek Correction: Evidence for plant-derived xenomiRs based on a large-scale analysis of public small RNA sequencing data from human samples
Článek Correction: Prognostic impact of systolic blood pressure variability in people with diabetes
Článek Retraction: Sialoglycosylation of RBC in Visceral Leishmaniasis Leads to Enhanced Oxidative Stress, Calpain-Induced Fragmentation of Spectrin and Hemolysis
Článek Too much? Mortality and health service utilisation among Danish children 1999-2016: A register-based study
Článek Effects of sodium-glucose cotransporter-2 inhibitors and dipeptidyl peptidase-4 inhibitors on diabetic retinopathy and its progression: A real-world Korean study
Článek Shipworm bioerosion of lithic substrates in a freshwater setting, Abatan River, Philippines: Ichnologic, paleoenvironmental and biogeomorphical implications
Článek Genetic codes optimized as a traveling salesman problem
Článek Effect of antenatal detection of small-for-gestational-age newborns in a risk stratified retrospective cohort
Článek Using family network data in child protection services
Článek Transcriptome sequencing reveals the effect of biochar improvement on the development of tobacco plants before and after topping
Článek Volatile anesthetics versus total intravenous anesthesia in patients undergoing coronary artery bypass grafting: An updated meta-analysis and trial sequential analysis of randomized controlled trials
Článek Cumulative fluid balance predicts mortality and increases time on mechanical ventilation in ARDS patients: An observational cohort study
Článek Stated-preference research in HIV: A scoping review
Článek Correction: Survival of infants ≤24 months of age with brain tumors: A population-based study using the SEER database
Článek Antinociceptive compounds and LC-DAD-ESIMSn profile from Dictyoloma vandellianum leaves
Článek Time series experimental design under one-shot sampling: The importance of condition diversity
Článek Evaluation of protocols for rRNA depletion-based RNA sequencing of nanogram inputs of mammalian total RNA
Článek Prevalence and predictors of primary postpartum hemorrhage: An implication for designing effective intervention at selected hospitals, Southern Ethiopia
Článek Correction: Stillbirths in urban Guinea-Bissau: A hospital- and community-based study
Článek Correction: Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging
Článek Correction: Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
Článek Correction: Novel site-specific PEGylated L-asparaginase
Článek Effect of caffeine ingestion on competitive rifle shooting performance
Článek Correction: Color adaptation induced from linguistic description of color
Článek MEK inhibition suppresses B regulatory cells and augments anti-tumor immunity
Článek Correction: A biface production older than 600 ka ago at Notarchirico (Southern Italy) contribution to understanding early Acheulean cognition and skills in Europe
Článek Correction: A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated extracellular vesicle enrichment by acoustic trapping to microRNA sequencing
Článek Measurement of horizontal ocular deviation on magnetic resonance imaging in various disease with acute vertigo
Článek Clinical outcomes of early gastric cancer with non-curative resection after pathological evaluation based on the expanded criteria
Článek Detection of quantitative trait loci associated with drought tolerance in St. Augustinegrass
Článek An examination of the Kuleshov effect using still photographs
Článek Correction: Relationship between non-communicable diseases and background characteristics among homeless people in Nagoya City, Japan
Článek An exploration of automated narrative analysis via machine learning
Článek Correction: Frozen by stress: Stress increases scope insensitivity
Článek The pragmatic role of trust in young children’s interpretation of unfamiliar signals
Článek Measuring egocentric distance perception in virtual reality: Influence of methodologies, locomotion and translation gains
Článek Mathematical modeling of cardiac function to evaluate clinical cases in adults and children
Článek Correction: Forecasting the impact of population ageing on tuberculosis incidence
Článek “The car is my extra legs” – Experiences of outdoor mobility amongst immigrants in Sweden with late effects of polio
Článek Correction: Multilevel analyses of on-demand medication data, with an application to the treatment of Female Sexual Interest/Arousal Disorder
Článek Correction: The world’s user-generated road map is more than 80% complete
Článek Correction: Predictors of opioid efficacy in patients with chronic pain: A prospective multicenter observational cohort study
Článek Correction: Comparative prognostic accuracy of sepsis scores for hospital mortality in adults with suspected infection in non-ICU and ICU at an academic public hospital
Článek Correction: Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant Isodon rubescens is catalyzed by a pair of diterpene synthases
Článek Correction: Effect of early measles vaccine on pneumococcal colonization: A randomized trial from Guinea-Bissau
Článek Correction: Tuberculosis patients in an Indian mega-city: Where do they live and where are they diagnosed?
Článek Correction: Priority Queue Based Reactive Buffer Management Policy for Delay Tolerant Network under City Based Environments
Článek Correction: Hedgehog inhibition mediates radiation sensitivity in mouse xenograft models of human esophageal adenocarcinoma
Článek Correction: A specific expression profile of LC3B and p62 is associated with nonresponse to neoadjuvant chemotherapy in esophageal adenocarcinomas
Článek Correction: Evolution and study of a copycat effect in intimate partner homicides: A lesson from Spanish femicides
Článek Correction: Transforming growth factor beta 1 induces methylation changes in lung fibroblasts
Článek Correction: Congenital Zika Syndrome in a Brazil-Paraguay-Bolivia border region: Clinical features of cases diagnosed between 2015 and 2018
Článek Correction: Celecoxib enhances the sensitivity of non-small-cell lung cancer cells to radiation-induced apoptosis through downregulation of the Akt/mTOR signaling pathway and COX-2 expression
Článek Correction: Factors influencing the behaviour and perceptions of Australian veterinarians towards antibiotic use and antimicrobial resistance
Článek Correction: Nonalcoholic fatty liver disease is a risk factor for large-for-gestational-age birthweight
Článek Retraction: Butyrate Attenuates Lipopolysaccharide-Induced Inflammation in Intestinal Cells and Crohn's Mucosa through Modulation of Antioxidant Defense Machinery
Článek Retraction: Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-κB and ERK Signaling Pathways in BV2 Cells
Článek Correction: Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
Článek Correction: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
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PLOS One

2019 Číslo 10
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Aktuální možnosti diagnostiky a léčby litiáz
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Autori: MUDr. Tomáš Ürge, PhD.
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