Acute ex vivo changes in brain white matter diffusion tensor metrics

English version

Autoři: Matthew R. Walker ^aff001; Jidan Zhong ^aff002; Adam C. Waspe ^aff003; Thomas Looi ^aff003; Karolina Piorkowska ^aff003; James M. Drake ^aff001; Mojgan Hodaie ^aff001
Působiště autorů: Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada ^aff001; Division of Brain, Imaging and Behaviour - Systems Neuroscience, Krembil Research Institute, University Health Network, Toronto, Ontario, Canada ^aff002; Centre for Image Guided Innovation and Therapeutic Intervention, Hospital for Sick Children, Toronto, Ontario, Canada ^aff003; Department of Medical Imaging, University of Toronto, Toronto, Ontario, Canada ^aff004; Division of Neurosurgery, Hospital for Sick Children, Toronto, Ontario, Canada ^aff005; Division of Neurosurgery, Toronto Western Hospital, University Health Network, Toronto, Ontario, Canada ^aff006
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0223211

Souhrn

Purpose

Diffusion magnetic resonance imaging and tractography has an important role in the visualization of brain white matter and assessment of tissue microstructure. There is a lack of correspondence between diffusion metrics of live tissue, ex vivo tissue, and histological findings. The objective of this study is to elucidate this connection by determining the specific diffusion alterations between live and ex vivo brain tissue. This may have an important role in the incorporation of diffusion imaging in ex vivo studies as a complement to histological sectioning as well as investigations of novel neurosurgical techniques.

Methods

This study presents a method of high angular resolution diffusion imaging and tractography of intact and non-fixed ex vivo piglet brains. Most studies involving ex vivo brain specimens have been formalin-fixed or excised from their original biological environment, processes both of which are known to affect diffusion parameters. Thus, non-fixed ex vivo tissue is used. A region-of-interest based analysis of diffusion tensor metrics are compared to in vivo subjects in a selection of major white matter bundles in order to assess the translatability of ex vivo diffusion measurements.

Results

Tractography was successfully achieved in both in vivo and ex vivo groups. No significant differences were found in tract connectivity, average streamline length, or apparent fiber density. Significantly decreased diffusivity (mean, axial, and radial; p<0.0005) in the non-fixed ex vivo group and unaltered fractional anisotropy (p>0.059) between groups were observed.

Conclusion

This study validates the extrapolation of non-fixed fractional anisotropy measurements to live tissue and the potential use of ex vivo tissue for methodological development.

Klíčová slova:

corpus callosum – Diffusion tensor imaging – Tractography – Central nervous system – Nerve fibers – Swine – Diffusion magnetic resonance imaging – Anisotropy

Zdroje

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