Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data

English version

Autoři: Tea Arabuli ^aff001; Mohamed Waleed Negm ^aff001; Tomoko Matsuda ^aff001; Yasuki Kitashima ^aff001; Tea Abramishvili ^aff006; Igor Andrijovych Akimov ^aff007; Olga Valentynivna Zhovnerchuk ^aff007; Sergei Yakovlevich Popov ^aff008; Tetsuo Gotoh ^aff001
Působiště autorů: Laboratory of Applied Entomology and Zoology, Faculty of Agriculture, Ibaraki University, Ami, Ibaraki, Japan ^aff001; Institute of Zoology, Ilia State University, Kakutsa Cholokashvilli Ave, Tbilisi, Georgia ^aff002; Institute of Entomology, Agricultural University of Georgia, Kakha Bendukidze Campus, David Aghamashenebeli Alley, Tbilisi, Georgia ^aff003; Department of Plant Protection, Faculty of Agriculture, Assiut University, Assiut, Egypt ^aff004; Japan Society for the Promotion of Science, Chiyoda, Tokyo, Japan ^aff005; Department of Plant Pest Diagnostic, Laboratory of the Ministry of Agriculture, Vasil Godziashvili, Tbilisi, Georgia ^aff006; Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Vul. B. Khmelnytskogo, Kyiv, Ukraine ^aff007; Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya, Moscow, Russia ^aff008; Faculty of Economics, Ryutsu Keizai University, Ryugasaki, Ibaraki, Japan ^aff009
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0221951

Souhrn

The genus Amphitetranychus Oudemans (Tetranychidae) consists of only three species, A. quercivorus (Ehara & Gotoh), A. savenkoae (Reck) and A. viennensis (Zacher). The original description of A. savenkoae was extremely simple and had no drawing of the aedeagus; however, a subsequent study described only the aedeagus. The present study investigated all three species in detail using a combination of morphological traits, crossbreeding experiments, esterase zymograms and the mitochondrial cytochrome c oxidase subunit I (COI) gene. Morphological differences in the peritremes and male aedeagi were observed among the three species. Complete reproductive isolation was confirmed in the reciprocal crosses between the morphologically similar A. savenkoae and A. quercivorus (no female offspring were produced). Esterase zymograms differed interspecifically, but not intraspecifically (among individuals in a given species). All three species formed clearly separate clades with 100% bootstrap values in the COI tree, and A. savenkoae was more closely related to A. quercivorus than to A. viennensis, which corresponded to the morphological similarity of their aedeagi and setal counts on tarsi IV. A key to Amphitetranychus species is provided.

Klíčová slova:

Biology and life sciences – Cell biology – Biochemistry – Plant science – Organisms – Eukaryota – Research and analysis methods – Enzymology – Enzymes – Proteins – Database and informatics methods – Bioinformatics – Sequence analysis – Animals – Invertebrates – Arthropoda – People and places – Computer and information sciences – Taxonomy – Data management – Geographical locations – Anatomy – Medicine and health sciences – Cellular structures and organelles – Plant anatomy – Leaves – Bioenergetics – Energy-producing organelles – Mitochondria – Musculoskeletal system – Body limbs – Sequence databases – Biological databases – Asia – Legs – Hydrolases – Esterases – Mites – Japan

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Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data

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