Genome-wide analysis of DNA methylation profile identifies differentially methylated loci associated with human intervertebral disc degeneration
Autoři:
Akihiro Ikuno aff001; Koji Akeda aff002; Shin-ichiro Takebayashi aff001; Motomu Shimaoka aff003; Katsuzumi Okumura aff001; Akihiro Sudo aff002
Působiště autorů:
Laboratory of Molecular & Cellular Biology, Graduate School of Bioresources, Mie University, Tsu, Japan
aff001; Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
aff002; Department of Molecular Pathobiology3, Mie University Graduate School of Medicine, Tsu, Japan
aff003
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222188
Souhrn
Background
Environmental and endogenous factors under genetic predisposition are considered to initiate the human intervertebral disc (IVD) degeneration. DNA methylation is an essential mechanism to ensure cell-specific gene expression for normal development and tissue stability. Aberrant epigenetic alterations play a pivotal role in several diseases, including osteoarthritis. However, epigenetic alternations, including DNA methylation, in IVD degeneration have not been evaluated. The purpose of this study was to comprehensively compare the genome-wide DNA methylation profiles of human IVD tissues, specifically nucleus pulpous (NP) tissues, with early and advanced stages of disc degeneration.
Methods
Human NP tissues were used in this study. The samples were divided into two groups: early stage degeneration (n = 8, Pfirrmann’s MRI grade: I-III) and advanced stage degeneration (n = 8, grade: IV). Genomic DNA was processed for genome-wide DNA methylation profiling using the Infinium MethylationEPIC BeadChip array. Extraction of raw methylation data, clustering and scatter plot of each group values of each sample were performed using a methylation module in GenomeStudio software. The identification of differentially methylated loci (DMLs) and the Gene Ontology (GO) analysis were performed using R software with the ChAMP package.
Results
Unsupervised hierarchical clustering revealed that early and advanced stage degenerated IVD samples segregated into two main clusters by their DNA methylome. A total of 220 DMLs were identified between early and advanced disc degeneration stages. Among these, four loci were hypomethylated and 216 loci were hypermethylated in the advanced disc degeneration stage. The GO enrichment analysis of genes containing DMLs identified two significant GO terms for biological processes, hemophilic cell adhesion and cell-cell adhesion.
Conclusions
We conducted a genome-wide DNA methylation profile comparative study and observed significant differences in DNA methylation profiles between early and advanced stages of human IVD degeneration. These results implicate DNA methylation in the process of human IVD degeneration.
Klíčová slova:
DNA – Biology and life sciences – Cell biology – Chromosome biology – Chromatin – Chromatin modification – DNA methylation – Genetics – Epigenetics – DNA modification – Gene expression – Genomics – Genome analysis – Biochemistry – Nucleic acids – Computational biology – Research and analysis methods – Genetic loci – Medicine and health sciences – Cellular structures and organelles – Diagnostic medicine – Rheumatology – Arthritis – Imaging techniques – Diagnostic radiology – Magnetic resonance imaging – Radiology and imaging – Signal transduction – Cell signaling – Signaling cascades – Wnt signaling cascade – Hedgehog signaling – Extracellular matrix – Gene ontologies – Osteoarthritis
Zdroje
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