Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)

English version

Autoři: Daniel J. Sargent ^aff001; Matteo Buti ^aff003; Nada Šurbanovski ^aff001; May Bente Brurberg ^aff004; Muath Alsheikh ^aff005; Matthew P. Kent ^aff007; Jahn Davik ^aff004
Působiště autorů: PlantSci Consulting Ltd. Kent, United Kingdom ^aff001; Fondazione Edmund Mach, San Michele all’Adige, Trentino, Italy ^aff002; Department of Agriculture, Food, Environment and Forestry, University of Florence, Florence, Italy ^aff003; Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, Norway ^aff004; Department of Plant Sciences, Norwegian University of Life Sciences, Ås, Norway ^aff005; Graminor Breeding Ltd., Ridabu, Norway ^aff006; Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway ^aff007
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0222829

Souhrn

Strawberry powdery mildew (Podosphaera aphanis Wallr.) is a pathogen which infects the leaves, fruit, stolon and flowers of the cultivated strawberry (Fragaria ×ananassa), causing major yield losses, primarily through unmarketable fruit. The primary commercial control of the disease is the application of fungicidal sprays. However, as the use of key active ingredients of commercial fungicides is becoming increasingly restricted, interest in developing novel strawberry cultivars exhibiting resistance to the pathogen is growing rapidly. In this study, a mapping population derived from a cross between two commercial strawberry cultivars (‘Sonata’ and ‘Babette’) was genotyped with single nucleotide polymorphism (SNP) markers from the Axiom iStraw90k genotyping array and phenotyped for powdery mildew susceptibility in both glasshouse and field environments. Three distinct, significant QTLs for powdery mildew resistance were identified across the two experiments. Through comparison with previous studies and scrutiny of the F. vesca genome sequence, candidate genes underlying the genetic control of this trait were identified.

Klíčová slova:

Biology and life sciences – Genetics – Genomics – Heredity – Plant science – Research and analysis methods – Molecular biology – Plant pathology – Plant pathogens – Plant fungal pathogens – Database and informatics methods – Bioinformatics – Sequence analysis – Genetic loci – Quantitative trait loci – Molecular biology techniques – Gene mapping – Plant anatomy – Leaves – Genetic linkage – Powdery mildew – Linkage mapping

Zdroje

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Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)

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