Levels of serum eosinophil cationic protein are associated with hookworm infection and intensity in endemic communities in Ghana
Autoři:
Benjamin Amoani aff001; Bright Adu aff002; Margaret T. Frempong aff003; Tracy Sarkodie-Addo aff002; Samuel Victor Nuvor aff004; Michael D. Wilson aff005; Ben Gyan aff002
Působiště autorů:
Department of Biomedical Science, College of Health Sciences, University of Cape Coast, Cape Coast, Ghana
aff001; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana
aff002; Molecular Medicine Department, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Ghana
aff003; Department of Microbiology, College of Health Sciences, University of Cape Coast, Cape Coast, Ghana
aff004; Parasitology Department, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana
aff005
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222382
Souhrn
Background
The eosinophil cationic protein (ECP) is a cytotoxic protein mainly secreted by eosinophils granulocytes and plays a role in host defense against parasitic infections. Infection with Necator americanus (hookworm) is traditionally diagnosed by the Kato-Katz method which is inherently tedious, subjective and known to underestimate infection intensity. This study aimed to assess levels of serum ECP in relation to hookworm infection intensity.
Methods
Stool samples from 984 (aged 4 to 80 years) participants in a cross-sectional study conducted in the Kintampo North Municipality of Ghana were examined using the Kato-Katz and formol-ether concentration methods. Serum ECP levels were measured by ECP assay kit and compared between 40 individuals infected with hookworm only, 63 with hookworm- Plasmodium falciparum co-infection, 59 with P. falciparum infection and 36 with no infection.
Results
Hookworm infection prevalence was 18.1% (178/984). ECP levels were significantly higher in individuals infected with hookworm only (β = 2.96, 95%CI = 2.69, 3.23, p<0.001) or co-infected with P. falciparum (β = 3.15, 95%CI = 2.91, 3.39, p<0.001) compared to the negative control. Levels of ECP were similar between those with only P. falciparum infection and the uninfected control (p>0.05). Increased hookworm intensity was associated with a significant increase in ECP level (β = 4.45, 95%CI = 2.25, 9.11, rs = 0.193, n = 103, p<0.01). ECP threshold of 84.98ng/ml was associated with a positive predictive value (PPV) of 98% (95% CI = 92, 100), and negative predictive value (NPV) of 76% (95% CI = 62, 87) in classifying hookworm infection status with an AUROC of 96.3%.
Conclusion
Serum ECP level may be a good biomarker of hookworm infection and intensity and warrant further investigations to help improve current hookworm diagnosis.
Klíčová slova:
Biology and life sciences – Cell biology – Organisms – Eukaryota – Research and analysis methods – Animals – Invertebrates – Parasitology – Cellular types – Animal cells – Medicine and health sciences – Nematoda – Infectious diseases – Immunology – Blood cells – White blood cells – Immune cells – Parasitic diseases – Immunologic techniques – Immunoassays – Enzyme-linked immunoassays – Helminths – Hookworms – Necator americanus – Necator – Eosinophils – Parasite groups – Apicomplexa – Plasmodium – Helminth infections – Co-infections
Zdroje
1. De Silva NR, Brooker S, Hotez PJ, Montresor A, Engels D, Savioli L. Soil-transmitted helminth infections: updating the global picture. Trends in parasitology. 2003;19(12):547–51. 14642761
2. Humphries D, Mosites E, Otchere J, Twum WA, Woo L, Jones-Sanpei H, et al. Epidemiology of hookworm infection in Kintampo North Municipality, Ghana: patterns of malaria coinfection, anemia, and albendazole treatment failure. The American journal of tropical medicine and hygiene. 2011;84(5):792–800. doi: 10.4269/ajtmh.2011.11-0003 21540391
3. Loukas A, Prociv P. Immune responses in hookworm infections. Clinical microbiology reviews. 2001;14(4):689–703. doi: 10.1128/CMR.14.4.689-703.2001 11585781
4. Dickson R, Awasthi S, Williamson P, Demellweek C, Garner P. Effects of treatment for intestinal helminth infection on growth and cognitive performance in children: systematic review of randomised trials. Bmj. 2000;320(7251):1697–701. doi: 10.1136/bmj.320.7251.1697 10864543
5. Ndyomugyenyi R, Kabatereine N, Olsen A, Magnussen P. Efficacy of ivermectin and albendazole alone and in combination for treatment of soil-transmitted helminths in pregnancy and adverse events: a randomized open label controlled intervention trial in Masindi district, western Uganda. The American journal of tropical medicine and hygiene. 2008;79(6):856–63. 19052293
6. Stoltzfus RJ, Kvalsvig JD, Chwaya HM, Montresor A, Albonico M, Tielsch JM, et al. Effects of iron supplementation and anthelmintic treatment on motor and language development of preschool children in Zanzibar: double blind, placebo controlled study. Bmj. 2001;323(7326):1389. doi: 10.1136/bmj.323.7326.1389 11744561
7. Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, et al. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. The Lancet. 2006;367(9521):1521–32.
8. Crompton DWT, Nesheim M. Nutritional impact of intestinal helminthiasis during the human life cycle. Annual review of nutrition. 2002;22(1):35–59.
9. Humphries D, Nguyen S, Kumar S, Quagraine JE, Otchere J, Harrison LM, et al. Effectiveness of Albendazole for Hookworm Varies Widely by Community and Correlates with Nutritional Factors: A Cross-Sectional Study of School-Age Children in Ghana. The American journal of tropical medicine and hygiene. 2017;96(2):347–54. doi: 10.4269/ajtmh.16-0682 27895280
10. Bystrom J, Amin K, Bishop-Bailey D. Analysing the eosinophil cationic protein-a clue to the function of the eosinophil granulocyte. Respiratory research. 2011;12(1):1.
11. Booth M, Vounatsou P, N'goran E, Tanner M, Utzinger J. The influence of sampling effort and the performance of the Kato-Katz technique in diagnosing Schistosoma mansoni and hookworm co-infections in rural Côte d'Ivoire. Parasitology. 2003;127(06):525–31.
12. Reimert C, Venge P, Kharazmi A, Bendtzen K. Detection of eosinophil cationic protein (ECP) by an enzyme-linked immunosorbent assay. Journal of immunological methods. 1991;138(2):285–90. 2033280
13. Niedworok M, Sordyl B, Borecka A, Gawor J, Małecka-Panas E. Estimation of eosinophilia, immunoglobulin E and eosinophilic cationic protein concentration during the treatment of toxocariasis. Wiadomosci parazytologiczne. 2007;54(3):225–30.
14. Asuming-Brempong E, Gyan B, Amoah AS, Puije Wvd, Bimi L, Boakye D, et al. Relationship between eosinophil cationic protein and infection intensity in a Schistosomiasis endemic community in Ghana. Research and Reports in Tropical Medicine. 2015;6:1.
15. Parham. The Immune system (2nd Edition); Taylor and Francis;. Abingdon, England, UK. 2004:318–9.
16. WHO. 'Guidelines for the evaluation of soil-transmitted helminthiasis and schistosomiasis atcommunity level. World Health Organization, Geneva. 1998.
17. Amoani B, Gyan B, Armah FA, Otabil C, Tchum K, Frempong MT, et al. Comparative Analysis of Haematological Parameters in Hookworm and Plasmodium falciparum Co-Infected Individuals in Kintampo North Municipality, Ghana. International Journal of TROPICAL DISEASE & Health. 2018:1–9.
18. Shete PB. The immunoepidemiology of Hookworm infection in the Peruvian Amazon. elischolar.library.yale.edu. 2008.
19. Zhan B, Li T, Xiao S, Zheng F, Hawdon J. Species-specific identification of human hookworms by PCR of the mitochondrial cytochrome oxidase I gene. Journal of Parasitology. 2001;87(5):1227–9. 11695411
20. Tischendorf F, Brattig N, Büttner D, Pieper A, Lintzel M. Serum levels of eosinophil cationic protein, eosinophil-derived neurotoxin and myeloperoxidase in infections with filariea and schistosomes. Acta tropica. 1996;62(3):171–82. 9025985
21. Hassan M, El-Motaim M, Mattar M, Afify H, el-Badrawy e-S. Assessing the morbidity of schistosomiasis by measuring eosinophil cationic protein in serum. Journal of the Egyptian Society of Parasitology. 2002;32(2):517–24. 12214929
22. Sugai T, Sakiyama Y, Matumoto S. Eosinophil cationic protein in peripheral blood of pediatric patients with allergic diseases. Clinical & Experimental Allergy. 1992;22(2):275–81.
23. Slobodna MS, Jasna L, Vesna Ž, Karmela H, Branka M. Serum eosinophil cationic protein in children with atopic dermatitis. International journal of dermatology. 2006;45(10):1156–60. 17040428
24. Bethony J, Loukas A, Smout M, Brooker S, Mendez S, Plieskatt J, et al. Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. The FASEB journal. 2005;19(12):1743–5. 16037096
25. Reimert CM, Fitzsimmons CM, Joseph S, Mwatha JK, Jones FM, Kimani G, et al. Eosinophil activity in Schistosoma mansoni infections in vivo and in vitro in relation to plasma cytokine profile pre-and posttreatment with praziquantel. Clinical and Vaccine Immunology. 2006;13(5):584–93. doi: 10.1128/CVI.13.5.584-593.2006 16682480
Článok vyšiel v časopise
PLOS One
2019 Číslo 9
- Metamizol jako analgetikum první volby: kdy, pro koho, jak a proč?
- Nejasný stín na plicích – kazuistika
- Masturbační chování žen v ČR − dotazníková studie
- Těžké menstruační krvácení může značit poruchu krevní srážlivosti. Jaký management vyšetření a léčby je v takovém případě vhodný?
- Fixní kombinace paracetamol/kodein nabízí synergické analgetické účinky
Najčítanejšie v tomto čísle
- Graviola (Annona muricata) attenuates behavioural alterations and testicular oxidative stress induced by streptozotocin in diabetic rats
- CH(II), a cerebroprotein hydrolysate, exhibits potential neuro-protective effect on Alzheimer’s disease
- Comparison between Aptima Assays (Hologic) and the Allplex STI Essential Assay (Seegene) for the diagnosis of Sexually transmitted infections
- Assessment of glucose-6-phosphate dehydrogenase activity using CareStart G6PD rapid diagnostic test and associated genetic variants in Plasmodium vivax malaria endemic setting in Mauritania