Characterization of melanin and optimal conditions for pigment production by an endophytic fungus, Spissiomyces endophytica SDBR-CMU319

English version

Autoři: Nakarin Suwannarach ^aff001; Jaturong Kumla ^aff001; Bunta Watanabe ^aff003; Kenji Matsui ^aff004; Saisamorn Lumyong ^aff001
Působiště autorů: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand ^aff001; Center of Excellence in Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai, Thailand ^aff002; Institute for Chemical Research, Kyoto University, Kyoto, Japan ^aff003; Graduate School of Sciences and Technology for Innovation (Agriculture), Yamaguchi University, Yamaguchi, Japan ^aff004; Academy of Science, The Royal Society of Thailand, Bangkok, Thailand ^aff005
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0222187

Souhrn

Melanin is a natural pigment that is produced by filamentous fungi. In this study, the endophytic species, Spissiomyces endophytica (strain SDBR-CMU319), produced a brown-black pigment in the mycelia. Consequently, the pigment was extracted from the dried fungal biomass. This was followed by pigment purification, characterization and identification. Physical and chemical characteristics of the pigment showed acid precipitation, alkali solubilization, decolorization with oxidizing agents, and insolubility in most organic solvents and water. The pigment was confirmed as melanin based on ultraviolet-visible spectroscopy, Fourier-transform infrared, and electron paramagnetic resonance spectra analyses. The analyses of the elemental composition indicated that the pigment possessed a low percentage of nitrogen, and therefore, was not 3,4-dihydroxyphenylalanine melanin. Inhibition studies involving specific inhibitors, both tricyclazole and phthalide, and suggest that fungal melanin could be synthesized through the 1,8-dihydroxynaphthalene pathway. The optimum conditions for fungal pigment production from this species were investigated. The highest fungal pigment yield was observed in glucose yeast extract peptone medium at an initial pH value of 6.0 and at 25°C over three weeks of cultivation. This is the first report on the production and characterization of melanin obtained from the genus Spissiomyces.

Klíčová slova:

Biology and life sciences – Plant science – Organisms – Eukaryota – Physical sciences – Chemistry – Chemical reactions – Research and analysis methods – Fungi – Plant pathology – Plant pathogens – Plant fungal pathogens – Rice blast fungus – Chemical compounds – Materials science – Materials – Organic compounds – Carbohydrates – Monosaccharides – Organic chemistry – Agriculture – Spectrum analysis techniques – Glucose – Fungiculture – Pigments – Organic pigments – Melanin – Chemical precipitation – Electron spin resonance spectroscopy

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Characterization of melanin and optimal conditions for pigment production by an endophytic fungus, Spissiomyces endophytica SDBR-CMU319

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