Influence of the cultivation medium and pH on the pigmentation of Trichophyton rubrum
Autoři:
Oliver Blechert aff001; Hailin Zheng aff001; Xiaohui Zang aff001; Qiong Wang aff001; Weida Liu aff001
Působiště autorů:
Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People’s Republic of China
aff001; Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, Jiangsu, People's Republic of China
aff002; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China
aff003
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222333
Souhrn
Trichophyton rubrum is a human pathogenic fungus. As a dermatophyte it causes athlete's foot, fungal infection of nails, jock itch and ringworm. The pigmentation of T. rubrum is variable and can range from white or yellow to wine-red. We demonstrate that the pigmentation is strongly influenced by pH. Under alkaline conditions, T. rubrum has a red pigmentation, whereas at acid conditions, T. rubrum has a yellow pigmentation. Moreover, the color change immediately from yellow to red by adding NaOH and reverse immediately from red to yellow by adding HCl. We suggest that the chemical compound Xanthomegnin is responsible for red as well for yellow pigmentation in T. rubrum. To figure out, why T. rubrum has red pigmentation on Trichophyton medium, adjust to alkaline, but not on Synthetic-Complete medium, also adjusted to alkaline, we measure the pH of liquid media, adjusted to pH 3.5, 6 and 8, over a period of four weeks. The pH of both cultivation media changes significantly, with a maximum of five pH levels. Whereas the Trichophyton medium, initially adjusted to pH 8, stays alkaline, the pH of the Synthetic-Complete medium drops to acid conditions. The acidification of the SC medium and the alkalization of the Trichophyton medium explains the different pigment color of the T. rubrum colonies.
Klíčová slova:
Biology and life sciences – Plant science – Organisms – Eukaryota – Physical sciences – Chemistry – Fungi – Plant pathology – Plant pathogens – Plant fungal pathogens – Mycology – Medicine and health sciences – Chemical compounds – Microbiology – Medical microbiology – Microbial pathogens – Pathology and laboratory medicine – Pathogens – Materials science – Materials – Fungal pathogens – Infectious diseases – Agriculture – Fungal diseases – Fungiculture – Acids – Pigments
Zdroje
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