Exposure to lipopolysaccharide (LPS) reduces contractile response of small airways from GSTCD-/- mice

English version

Autoři: Bo Liu ^aff001; Amanda P. Henry ^aff001; Sheyda Azimi ^aff001; Suzanne Miller ^aff001; Frank K. Lee ^aff002; Jane C. Lee ^aff002; Kelly Probert ^aff001; Michael I. Kotlikoff ^aff002; Ian Sayers ^aff001; Ian P. Hall ^aff001
Působiště autorů: Division of Respiratory Medicine, & National Institute for Health Medicine, Nottingham Biomedical Research Centre, University of Nottingham, Nottingham, England, United Kingdom ^aff001; Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America ^aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0221899

Souhrn

Introduction

Genome-Wide Association Studies suggest glutathione S transferase C terminal domain (GSTCD) may play a role in development of Chronic Obstructive Pulmonary Disease. We aimed to define the potential role of GSTCD in airway inflammation and contraction using precision cut lung slice (PCLS) from wild-type (GSTCD^+/+) and GSTCD knockout mice (GSTCD^-/-).

Methods

PCLS from age and gender matched GSTCD^+/+ and GSTCD^-/- mice were prepared using a microtome. Contraction was studied after applying either a single dose of Methacholine (Mch) (1 μM) or different doses of Mch (0.001 to 100 μM). Each slice was then treated with lipopolysaccharide (LPS) or vehicle (PBS) for 24 hours. PCLS contraction in the same airway was repeated before and after stimulation. Levels of TNFα production was also measured.

Results

There were no differences in contraction of PCLS from GSTCD^+/+ and GSTCD^-/- mice in response to Mch (EC₅₀ of GSTCD^+/+ vs GSTCD^-/- animals: 100.0±20.7 vs 107.7±24.5 nM, p = 0.855, n = 6 animals/group). However, after LPS treatment, there was a 31.6% reduction in contraction in the GSTCD^-/- group (p = 0.023, n = 6 animals). There was no significant difference between PBS and LPS treatment groups in GSTCD^+/+ animals. We observed a significant increase in TNFα production induced by LPS in GSTCD^-/- lung slices compared to the GSTCD^+/+ LPS treated slices.

Conclusion

GSTCD knockout mice showed an increased responsiveness to LPS (as determined by TNFα production) that was accompanied by a reduced contraction of small airways in PCLS. These data highlight an unrecognised potential function of GSTCD in mediating inflammatory signals that affect airway responses.

Klíčová slova:

Biology and life sciences – Organisms – Eukaryota – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Animals – Animal models – Medicine and health sciences – Pathology and laboratory medicine – Vertebrates – Amniotes – Mammals – Diagnostic medicine – Signs and symptoms – Immunology – Immune response – Inflammation – Pulmonology – Imaging techniques – Mouse models – Immunologic techniques – Rodents – Mice – Diagnostic radiology – Radiology and imaging – Pulmonary function – Pulmonary imaging – Chronic obstructive pulmonary disease – Specimen preparation and treatment – Staining – Cell staining – Histochemistry and cytochemistry techniques – Immunohistochemistry techniques

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