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Additive and heterozygous (dis)advantage GWAS models reveal candidate genes involved in the genotypic variation of maize hybrids to Azospirillum brasilense


Autoři: Miriam Suzane Vidotti aff001;  Danilo Hottis Lyra aff002;  Júlia Silva Morosini aff001;  Ítalo Stefanine Correia Granato aff003;  Maria Carolina Quecine aff001;  João Lúcio de Azevedo aff001;  Roberto Fritsche-Neto aff001
Působiště autorů: Department of Genetics, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Piracicaba, São Paulo, Brazil aff001;  Rothamsted Research, Harpenden, Hertfordshire, England, United Kingdom aff002;  French National Institute for Agricultural Research, Montpellier, France aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0222788

Souhrn

Maize genotypes can show different responsiveness to inoculation with Azospirillum brasilense and an intriguing issue is which genes of the plant are involved in the recognition and growth promotion by these Plant Growth-Promoting Bacteria (PGPB). We conducted Genome-Wide Association Studies (GWAS) using additive and heterozygous (dis)advantage models to find candidate genes for root and shoot traits under nitrogen (N) stress and N stress plus A. brasilense. A total of 52,215 Single Nucleotide Polymorphism (SNP) markers were used for GWAS analyses. For the six root traits with significant inoculation effect, the GWAS analyses revealed 25 significant SNPs for the N stress plus A. brasilense treatment, in which only two were overlapped with the 22 found for N stress only. Most were found by the heterozygous (dis)advantage model and were more related to exclusive gene ontology terms. Interestingly, the candidate genes around the significant SNPs found for the maize–A. brasilense association were involved in different functions previously described for PGPB in plants (e.g. signaling pathways of the plant's defense system and phytohormone biosynthesis). Our findings are a benchmark in the understanding of the genetic variation among maize hybrids for the association with A. brasilense and reveal the potential for further enhancement of maize through this association.

Klíčová slova:

Biology and life sciences – Genetics – Gene expression – Genomics – Genome analysis – Heredity – Genetic mapping – Biochemistry – Plant science – Organisms – Eukaryota – Plants – Grasses – Maize – Computational biology – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Plant and algal models – Proteins – DNA-binding proteins – Molecular biology – Plant pathology – Gene regulation – Phenotypes – Molecular genetics – Genome-wide association studies – Human genetics – Transcription factors – Regulatory proteins – Hormones – Ecology and environmental sciences – Plant ecology – Plant-environment interactions – Ecology – Plant physiology – Plant defenses – Plant resistance to abiotic stress – Plant biochemistry – Variant genotypes – Plant hormones


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