Beta-defensins and analogs in Helicobacter pylori infections: mRNA expression levels, DNA methylation, and antibacterial activity

English version

Autoři: Raffaela Pero ^aff001; Tiziana Angrisano ^aff003; Mariarita Brancaccio ^aff004; Annarita Falanga ^aff005; Lucia Lombardi ^aff006; Francesco Natale ^aff003; Sonia Laneri ^aff005; Barbara Lombardo ^aff001; Stefania Galdiero ^aff005; Olga Scudiero ^aff001
Působiště autorů: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli “Federico II”, Napoli, Italy ^aff001; Task Force sugli Studi del Microbioma, Università degli Studi di Napoli “Federico II”, Napoli, Italy ^aff002; Dipartimento di Biologia, Università degli Studi di Napoli “Federico II”, Napoli, Italy ^aff003; Dipartimento di Biologia ed Evoluzione degli Organismi Marini, Stazione Zoologica Anton Dohrn, Napoli, Italy ^aff004; Dipartimento di Farmacia, Università degli Studi di Napoli “Federico II”, Napoli, Italy ^aff005; Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Napoli, Italy ^aff006; CEINGE-Biotecnologie Avanzate Scarl, Napoli, Italy ^aff007
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0222295

Souhrn

Antimicrobial peptides can protect the gastric mucosa from bacteria, but Helicobacter pylori (H. pylori) can equally colonize the gastric apparatus. To understand beta-defensin function in H. pylori-associated chronic gastritis, we investigated susceptibility, human beta-defensin mRNA expression, and DNA methylation changes to promoters in the gastric mucosa with or without H. pylori infection. We studied the expression of HBD2 (gene name DEFB4A), HBD3 (DEFB103A), and HBD4 (DEFB104) using real-time PCR in 15 control and 10 H. pylori infection patient gastric specimens. This study demonstrates that H. pylori infection is related to gastric enhancement of inducible HBD2, but inducible HBD3 and HBD4 expression levels remained unchanged. HBD2 gene methylation levels were overall higher in H. pylori-negative samples than in H. pylori-positive samples. We also assessed antimicrobial susceptibility using growth on blood agar. The H. pylori strain Tox+ was susceptible to all defensins tested and their analogs (3N, 3NI). These results show that HBD2 is involved in gastritis development driven by H. pylori, which facilitates the creation of an epigenetic field during H. pylori-associated gastric tumorigenesis.

Klíčová slova:

DNA – Helicobacter pylori – Biology and life sciences – Cell biology – Chromosome biology – Chromatin – Chromatin modification – DNA methylation – Genetics – Epigenetics – DNA modification – Gene expression – Biochemistry – Nucleic acids – Organisms – Research and analysis methods – Database and informatics methods – Bioinformatics – Sequence analysis – Medicine and health sciences – Microbiology – Medical microbiology – Microbial pathogens – Bacterial pathogens – Bacteria – Pathology and laboratory medicine – Pathogens – Diagnostic medicine – Signs and symptoms – Pharmacology – Immunology – Immune response – Inflammation – Gastroenterology and hepatology – Microbial control – Antimicrobials – Drugs – DNA sequence analysis – Helicobacter – Gastritis – Gastrointestinal infections

Zdroje

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Beta-defensins and analogs in Helicobacter pylori infections: mRNA expression levels, DNA methylation, and antibacterial activity

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