Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice
Autoři:
Franz Marielle Nogoy aff001; Yu Jin Jung aff002; Kwon-Kyoo Kang aff002; Yong-Gu Cho aff001
Působiště autorů:
Department of Crop Science, Chungbuk National University, Cheongju, Korea
aff001; Department of Horticulture, Hankyong National University, Ansung, Korea
aff002
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222262
Souhrn
Mutation breeding has brought significant contributions to the development of high value crops. It steered the first studies to generate plants with desired mutations of genes encoding key enzymes involved in important metabolic pathways. Molecular characterization of 5-methyl tryptophan (5-MT) resistant plants has revealed different base changes in alpha unit of anthranilate synthase (OsASA) gene that can lead to insensitivity to feedback inhibition of anthranilate synthase. The objective of this study was to perform in silico analysis of microarray data from five progressing time points during grain filling of rice. Results showed various differentially expressed genes. Enrichment of these genes revealed their roles in amino acid transportation during grain filling. Surprisingly, among all DEGs, only LOC_Os06g42560, a tryptophan synthase beta chain, was found to be directly related to tryptophan biosynthesis. It might affect amino acid content during grain filling. For physico-chemical analysis, different grain and eating qualities parameters were measured using mutant rice lines. Evaluation results showed that 5MT resistant-lines (5MT R-lines) showed approximately 60% chalkiness after milling although it had 20 times higher tryptophan content measured in μg/100 mg seeds. Taste quality of these 5MT R-lines in general was not affected significantly. However, other parameters such as peak time of viscosity and gelatinization temperature showed different results compared to the wildtype. Mutant lines generated in this study are important resources for high tryptophan content, although they have lower grain quality than the wildtype. They might be useful for developing new high nutrient rice varieties.
Klíčová slova:
Biology and life sciences – Genetics – Gene expression – Biochemistry – Organisms – Eukaryota – Plants – Grasses – Physical sciences – Chemistry – Research and analysis methods – Animal studies – Experimental organism systems – Plant and algal models – Proteins – Molecular biology – Rice – Molecular biology techniques – Mutation – Point mutation – Chemical compounds – Materials science – Amino acids – Molecular biology assays and analysis techniques – Organic compounds – Carbohydrates – Organic chemistry – Physics – Biosynthesis – Physical chemistry – Chemical properties – Materials physics – Viscosity – Amino acid analysis – Aromatic amino acids – Tryptophan – Starches
Zdroje
1. Kubo M, Purevdorj M (2004) The future of rice production and consumption. Journal of Food Distribution Research. 35(1): 128–142.
2. Moffett JR, Namboodiri MA (2003) Tryptophan and the immune response. Immunol Cell Biol. 81(4): 247–65. 12848846
3. Ishihara A, Matsuda F, Miyagawa H, Wakasa K (2007) Metabolomics for metabolically manipulated plants: effects of tryptophan overproduction. Metabolomics. 3: 319–334.
4. Ishikawa Y, Park J, Kisaka H, Lee H, Kanno A, Kameya T (2003) A 5-methyltryptophan resistant mutant of rice has an altered regulation of anthranilate synthase gene expression. Plant Science. 164(6): 1037–1045.
5. Ranch JP, Rick S, Brotherton JE, Widholm JM (1983) Expression of 5-Methyltryptophan resistance in plants regenerated from resistant cell lines of Datura innoxia. Plant Physiol. 71: 136–140. 16662772
6. Niyogi KK, Fink GR (1992) Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway. Plant Cell. 4: 721–733. doi: 10.1105/tpc.4.6.721 1392592
7. Kang KK, Kameya T (1993) Selection and characterization of a 5-methyltryptophan resistant mutant in Zea mays L. Euphytica. 69: 95.
8. Tam YY, Slovin JP, Cohen JD (1995) Selection and characterization of α-methyltryptophan-resistant lines of Lemna gibba showing a rapid rate of Indole-3-acetic acid turnover. Plant Physiol. 107: 77–85. 12228344
9. Cho HJ, Brotherton JE, Song HS, Widholm JM (2000) Increasing tryptophan synthesis in a forage legume Astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) Gene. Plant Physiol. 123: 1069–1076. 10889256
10. Zhang XH, Brotherton JE, Widholm JM, Portis AR Jr. (2001) Targeting a nuclear anthranilate synthase α-subunit gene to the tobacco plastid genome results in enhanced tryptophan biosynthesis. Return of a gene to its pre-endosymbiotic origin. Plant Physiol. 127: 131–141. 11553741
11. Hanafy MS, Rahman SM, Khalafalla M, El-Shemy HA, Nakamoto Y, Ishimoto M, et al. (2006) Accumulation of free tryptophan in Azuki bean (Vigna angularis) induced by expression of a gene (OASA1D) for a modified α-subunit of rice anthranilate synthase. Plant Science. 171: 670–676.
12. Lee HY and Kameya T (1991) Selection and characterization of a rice mutant resistant to 5-methyltryptophan. Theor Appl Genet. 82: 405–408. doi: 10.1007/BF00588590 24213253
13. Tozawa Y, Hasegawa H, Terakawa T, Wakasa K (2001) Characterization of rice anthranilate synthase alpha-subunit genes OASA1 and OASA2. Plant Physiology. 126(4): 1493–1506. 11500548
14. Kim D, Le I, Jang C, Kang S, Park I, Song H, et al. (2005) High amino acid accumulating 5-methyltryptophan-resistant rice mutants may include an increased antioxidative response system. Physiologia Plantarum. 123(3): 302–313.
15. Kanno T, Kasai K, Ikejiri-Kanno Y, Wakasa K, Tozawa Y (2004) In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the α subunits OASA1 and OASA2. Plant Molecular Biology. 54(1): 11–22. 15159631
16. Yamada T, Tozawa Y, Hasegawa H, Terakawa T, Ohkawa Y, Wakasa K (2004) Use of a feedback-insensitive subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. Molecular Breeding. 14: 363–373.
17. Kim DS, Kim JB, Lee GJ, Kang SY, Jang CS, Lee SY, et al. (2007) Identification of expressed sequence tags from a cDNA library of 5-Methyltryptophan resistant rice mutants. Journal of Radiation Industry. 1(1): 1–13.
18. Cho YG, Lee HJ, Nogoy FM, Nino MC. (2014) Development of high quality rice variety producing high content tryptophan by gene targeting technology. Agriculture, Forestry and Livestock 11-1543000-000753-01.
19. Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res. 16(22): 10881–10890. doi: 10.1093/nar/16.22.10881 2849754
20. Juliano BO (1971) A simplified assay for milled rice amylose. Cereal Science Today. 16: 334–338.
21. Kambhampati S, Li J, Evans BS, Allen DK (2019) Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry. Plant Methods. 15:46. doi: 10.1186/s13007-019-0430-z 31110556
22. Lee HJ, Jee MG, Kim JK, Nogoy FMC, Niño MC, Yu DA, et al. (2014) Modification of starch composition using RNAi targeting soluble starch synthase I in Japonica rice. Plant Breed. Biotech. 2: 301–312.
23. Nogoy FM, Jung YJ, Kang KK, Cho YG (2018) Characterization of ‘GolSam’ lines developed from the cross between Samgwang and 5MT resistant lines in rice. Plant Breed. Biotech. 6: 233–244.
24. Mi H, Thomas P (2009) Protein networks and pathway analysis. Methods in Molecular Biology. 563(2): 123–140.
25. Mi H, Muruganujan A, Casagrande JT, Thomas PD (2013) Large-scale gene function analysis with the PANTHER classification system. Nature Protocols. 8: 1551–1566. doi: 10.1038/nprot.2013.092 23868073
26. Mi H, Huang X, Muruganujan A, Tang H, Mills C, Kang D, et al. (2016) PANTHER version 11: expanded annotation data from gene ontology and reactome pathways, and data analysis tool enhancements. Nucl. Acids Res. 45(D1): D183–D189. doi: 10.1093/nar/gkw1138 27899595
27. Gene Ontology Consortium (2016) Expansion of the gene ontology knowledgebase and resources. Nucleic Acids Res. 45(D1): D331–D338. doi: 10.1093/nar/gkw1108 27899567
28. Lester G (1968) In vivo regulation of intermediate reactions in the pathway of tryptophan biosynthesis in Neurospora crassa. Journal of Bacteriology. 96: 1768–1773. 5726311
29. Marmorstein RQ, Sigler PB (1989) Stereochemical effects of L-Tryptophan and its analogues on trp repressor’s affinity for operator-DNA. Journal of Biological Chemistry. 264(16): 9149–9154. 2656696
30. Hyde EI, Ramesh V, Frederick R, Roberts GCK (1991) NMR studies of the activation of the Escherichia coli trp repressor. FEBS Journal. 201(3): 569–579.
31. Wakasa K, Hasegawa H, Nemoto H, Matsuda F, Miyazawa H, Tozawa Y, et al. (2006) High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. Journal of Experimental Botany. 57: 3069–3078. 16908506
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