Comparison between Aptima Assays (Hologic) and the Allplex STI Essential Assay (Seegene) for the diagnosis of Sexually transmitted infections

English version

Autoři: Adolfo de Salazar ^aff001; Beatriz Espadafor ^aff002; Ana Fuentes-López ^aff001; Antonio Barrientos-Durán ^aff001; Luis Salvador ^aff002; Marta Álvarez ^aff001; Federico García ^aff001
Působiště autorů: Hospital Universitario San Cecilio, Servicio de Microbiología, Instituto de Investigación Ibs, Granada, Spain ^aff001; Hospital Universitario Virgen de las Nieves, Servicio de Dermatología, Centro de ETS, Granada, Spain ^aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0222439

Souhrn

Sexually transmitted infections (STIs) remain a worldwide problem and a severe threat to public health. The purpose of this study was to compare Aptima^® Assays (Hologic^®) and the Allplex^™ STI Essential Assay (Seegene^®) for the simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium in clinical practice. The Aptima^® assays (Hologic^®) are based on a transcription-mediated amplification (TMA) method. The Allplex^™ STI Essential assay (Seegene^®) is based on a multiplex Real-Time PCR (RT-PCR) method. A total of 622 clinical samples from different anatomical sites were tested using both methods. A total of 88 (14.1%) and 66 (10.6%) positive samples were found for any of the TMA assays used and for the RT-PCR assay, respectively. Aptima^® assays showed a slightly higher rate of positive results for all pathogens except for T. vaginalis, the results of which were similar to those obtained with Allplex^™. The most commonly detected pathogen was C. trachomatis (37 samples; 5.9% using TMA assays) and the anatomical site with the highest prevalence of microorganisms was a non-urogenital site, the pharynx (27 positive samples; 4.3%). Using the Aptima^® assays as reference method, the comparison showed that the average specificity of multiplex RT-PCR was 100.0% for the four pathogens. However an average sensitivity of 74.5% was observed, showing 95.2% (CI95%; 93.6–96.9) of overall concordance (κ = 0.80). In conclusion, the Aptima^® assays show a higher sensitivity on a wide range of sample types compared to the Allplex^™ assay.

Klíčová slova:

Biology and life sciences – Biochemistry – Nucleic acids – Organisms – Eukaryota – Research and analysis methods – Molecular biology – Molecular biology techniques – Anatomy – Medicine and health sciences – Microbiology – Medical microbiology – Microbial pathogens – Bacterial pathogens – Bacteria – Pathology and laboratory medicine – Pathogens – Physiology – Infectious diseases – Body fluids – Artificial gene amplification and extension – Polymerase chain reaction – Sexually transmitted diseases – Reverse transcriptase-polymerase chain reaction – Urine – Chlamydia – Neisseria – Protists – Trichomonas – Trichomonas vaginalis – Chlamydia trachomatis – Neisseria gonorrhoeae – Chlamydia infection

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Comparison between Aptima Assays (Hologic) and the Allplex STI Essential Assay (Seegene) for the diagnosis of Sexually transmitted infections

Souhrn

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Zdroje

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