Comparison of post-traumatic changes in circulating and bone marrow leukocytes between BALB/c and CD-1 mouse strains
Autoři:
Tanja Spenlingwimmer aff001; Johannes Zipperle aff001; Mohammad Jafarmadar aff001; Marcin Filip Osuchowski aff001; Susanne Drechsler aff001
Působiště autorů:
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Vienna, Austria
aff001
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222594
Souhrn
This manuscript emerged from a larger third-party funded project investigating a new poly-trauma model and its influence upon secondary sepsis. The present sub-study compared selected leukocyte subpopulations in the circulation and bone marrow after polytrauma in BALB/c versus CD-1 mice. Animals underwent unilateral femur fracture, splenectomy and hemorrhagic shock. We collected blood and bone marrow for flow cytometry analysis at 24h and 48h post-trauma. Circulating granulocytes (Ly6G+CD11+) increased in both strains after trauma. Only in BALB/c mice circulating CD8+ T-lymphocytes decreased within 48h by 30%. Regulatory T-cells (Tregs, CD4+CD25+CD127low) increased in both strains by approx. 32%. Circulating Tregs and lymphocytes (CD11b-Ly6G-MHC-2+) were always at least 1.5-fold higher in BALB/c, while the bone marrow MHC-2 expression decreased in CD-1 mice (p<0.05). Overall, immune responses to polytrauma were similar in both strains. Additionally, BALB/c expressed higher level of circulating regulatory T-cells and MHC-2-positive lymphocytes compared to CD-1 mice.
Klíčová slova:
Biology and life sciences – Cell biology – Genetics – Heredity – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Animal models – Cellular types – Animal cells – Anatomy – Medicine and health sciences – Physiology – Immunology – Immune system – Immune physiology – Body fluids – Blood – Blood cells – White blood cells – T cells – Immune cells – Mouse models – Inbred strains – Lymphocytes – Bone marrow – Inbreeding
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