Time-related immunomodulation by stressors and corticosterone transdermal application in toads

English version

Autoři: Stefanny Christie Monteiro Titon ^aff001; Braz Titon, Jr ^aff001; Adriana Maria Giorgi Barsotti ^aff001; Fernando Ribeiro Gomes ^aff001; Vania Regina Assis ^aff001
Působiště autorů: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil ^aff001
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0222856

Souhrn

Immune responses have been mostly studied at a specific time in anuran species. However, time-changes related to immunomodulation associated with glucocorticoid (GC) alterations following stressors and GC treatment are complex. The present study describes time-related changes in immune response and corticosterone (CORT) plasma levels following restraint challenge, short, mid and long-term captivity, and CORT exogenous administration by transdermal application (TA) in Rhinella ornata toads. We observed increased neutrophil: lymphocyte ratios after restraint challenge and CORT TA, without changes following short and mid-term captivity. Plasma bacterial killing ability was sustained in all treatments, except long-term captivity, with decreased values after 90 days under such conditions. Phagocytic activity of peritoneal cells increased after mid-term captivity, and the phytohemagglutinin swelling response was impaired in those animals treated with CORT TA for 20 consecutive days. Plasma CORT levels increased or were sustained after restraint challenge (depending on initial values), decreased following mid and long-term captivity (for those animals showing high CORT in the field) and increased after 20 days of CORT TA. By performing assessments of time-changes in immune processes and CORT plasma levels in R. ornata, we demonstrate immuno-enhancing effects following restraint, short and mid-term stressors, while long-term stressors and CORT TA promoted immunosuppression in these toads.

Klíčová slova:

Biology and life sciences – Cell biology – Organisms – Eukaryota – Cell processes – Animals – Cellular types – Animal cells – Anatomy – Medicine and health sciences – Pathology and laboratory medicine – Physiology – Vertebrates – Diagnostic medicine – Signs and symptoms – Immunology – Immune response – Body fluids – Blood – Blood plasma – Blood cells – White blood cells – Immune cells – Amphibians – Lymphocytes – Cellular stress responses – Edema – Neutrophils – Phagocytosis – Toads

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Time-related immunomodulation by stressors and corticosterone transdermal application in toads

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