Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections
Autoři:
Theresa Schwaiger aff001; Julia Sehl aff001; Claudia Karte aff003; Alexander Schäfer aff004; Jane Hühr aff004; Thomas C. Mettenleiter aff002; Charlotte Schröder aff001; Bernd Köllner aff004; Reiner Ulrich aff001; Ulrike Blohm aff004
Působiště autorů:
Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany
aff001; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany
aff002; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany
aff003; Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany
aff004; Institute of Veterinary Pathology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany
aff005
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
prolekare.web.journal.doi_sk:
https://doi.org/10.1371/journal.pone.0222943
Souhrn
Pigs are anatomically, genetically and physiologically comparable to humans and represent a natural host for influenza A virus (IAV) infections. Thus, pigs may represent a relevant biomedical model for human IAV infections. We set out to investigate the systemic as well as the local immune response in pigs upon two subsequent intranasal infections with IAV H1N1pdm09. We detected decreasing numbers of peripheral blood lymphocytes after the first infection. The simultaneous increase in the frequencies of proliferating cells correlated with an increase in infiltrating leukocytes in the lung. Enhanced perforin expression in αβ and γδ T cells in the respiratory tract indicated a cytotoxic T cell response restricted to the route of virus entry such as the nose, the lung and the bronchoalveolar lavage. Simultaneously, increasing frequencies of CD8αα expressing αβ T cells were observed rapidly after the first infection, which may have inhibited uncontrolled inflammation in the respiratory tract. Taking together, the results of this study demonstrate that experimental IAV infection in pigs mimics major characteristics of human seasonal IAV infections.
Klíčová slova:
Biology and life sciences – Cell biology – Organisms – Eukaryota – Animals – Cellular types – Animal cells – Anatomy – Medicine and health sciences – Microbiology – Medical microbiology – Microbial pathogens – Pathology and laboratory medicine – Pathogens – Vertebrates – Amniotes – Mammals – Viral pathogens – Viruses – RNA viruses – Immunology – Immune response – Pulmonology – Respiratory infections – Blood cells – White blood cells – T cells – Cytotoxic T cells – Immune cells – Orthomyxoviruses – Influenza viruses – Influenza A virus – Head – Face – Swine – Lymphatic system – Lymph nodes – Nose
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