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preLekára.sk / Odborné časopisy / PLOS One / 2019 - 9

Sequential two-step chromatographic purification of infectious poliovirus using ceramic fluoroapatite and ceramic hydroxyapatite columns

Authors: Yae Kurosawa aff001;  Shigehiro Sato aff002;  Tsuneo Okuyama aff001;  Masato Taoka aff004
Authors place of work: R&D Department, HOYA Technosurgical Corporation, Akishima-shi, Tokyo, Japan aff001;  Laboratory of Infectious Disease and Immunology, Department of Microbiology, Iwate Medical University, Shiwa, Iwate, Japan aff002;  Protein Technos Institute, Atsugi-shi, Kanagawa, Japan aff003;  Laboratory of Biophysics and Biochemistry, Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan aff004
Published in the journal: PLoS ONE 14(9)
Category: Research Article
doi: https://doi.org/10.1371/journal.pone.0222199

Summary

Infectious virus purification techniques are important for vaccine development and gene therapy applications. However, the standardized one-step purification technique using ceramic hydroxyapatite (CHAp) has proven unsuitable for poliovirus. Therefore, we designed a sequential two-step chromatographic technique for purification of the infectious Sabin type 2 vaccine strain of poliovirus from the cell culture supernatant. In the first step, we removed protein contaminants from the Sabin type 2 virus fraction by pH gradient elution on a ceramic fluoroapatite column. In the second step, we removed double-stranded DNA derived from host cells by diluting the virus fraction, directly loading it on a CHAp column, and purifying it using a phosphate gradient with 1 M sodium chloride. This process achieved removal rates of more than 99.95% and 99.99% for proteins and double-stranded DNA, respectively, and was highly reproducible and scalable. Furthermore, it is likely that it will be applicable to other virus species.

Keywords:

Biology and life sciences – Organisms – Physical sciences – Chemistry – Research and analysis methods – Medicine and health sciences – Chemical compounds – Phosphates – Microbiology – Medical microbiology – Microbial pathogens – Pathology and laboratory medicine – Pathogens – Materials science – materials – infectious diseases – Viral pathogens – viruses – RNA viruses – Flaviviruses – Infectious disease control – Vaccines – Orthomyxoviruses – Influenza viruses – Biological cultures – Cell cultures – Dengue virus – Separation processes – Elution – ceramics – Sodium phosphate – Enteroviruses – Poliovirus

Introduction

The development of safer vaccines demands a reduction in their side effects; hence, regulatory authorities such as the Food and Drug Administration require both developers and manufacturers to improve the production and purification processes [1,2]. Various procedures have been developed for vaccine purification, including ultracentrifugation, liquid chromatography (LC), electrophoresis, precipitation, and molecular sieving. In particular, ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients has been used to process several viral preparations for pharmaceutical use [3]. However, this procedure decreases the infectivity of some viruses [4] and is unable to separate particulate contaminants with physical characteristics similar to the virus particles, such as host cell DNA, from the virus fraction [5]. In addition, density-gradient separation is time-consuming and has low scalability and economic efficiency.

There is a current trend toward using LC for the purification of viral preparations because it is easy to use from the early capture stage to the final purification phase, and, unlike traditional centrifugal procedures, offers a straightforward scale-up. Furthermore, it has recently been used for large-scale purification (>1013 virus particles) using several approaches, including ion exchange, size exclusion, hydrophobic interaction, immobilized metal affinity, and hydroxyapatite chromatography [6–13].

For biosafety reasons, hydroxyapatite is commonly used in biomaterial engineering and regenerative medicine [14] as well as for the purification of pharmaceutical products [15]. Recently, our team standardized the hydroxyapatite chromatography procedure and successfully purified dengue virus type 2 and Japanese encephalitis virus from the cell culture supernatants of virus-infected C6/36 cells and mouse brain homogenate, respectively, using one-step hydroxyapatite LC [16,17]. Scanning electron microscopy analysis confirmed that the virus particles were bound to and released from the surface of the hydroxyapatite via the chromatographic processes, clearly indicating the phosphate-dependent absorption/desorption mechanism of this material [18,19]. Therefore, we considered that apatite-based materials would be suitable for the purification of vaccines for diseases and vectors for gene therapies, increasing their effectiveness and reducing their cost.

Two kinds of polio vaccines are currently available: the Salk and Sabin vaccines. The Salk vaccine is an injectable vaccine of formalin-inactivated virulent poliovirus strains, whereas the Sabin vaccine is an oral vaccine that uses live attenuated virus strains. The Sabin oral vaccine causes occasional incidences of vaccine-associated paralytic poliomyelitis by a circulating vaccine-derived poliovirus [20]; hence, the development of a noninfectious method of vaccination has become a priority. Consequently, an injectable Sabin vaccine has recently been developed from safer strains [21,22], and it is now possible for small companies to manufacture this vaccine with a smaller investment in facilities. This is because the Sabin strain is less transmissible than the other wild-type strains and does not require a high level of biosafety [23]. Polioviruses belong to acid-stable Picornaviridae and retain their infectivity at pH 3 and lower [24]. The spherical virus particles do not contain a lipid envelope, have a diameter of approximately 30 nm, and are composed of four structural proteins: VP1, VP2, VP3, and VP4. However, the standardized one-step virus purification procedure using ceramic hydroxyapatite (CHAp) is unsuitable for poliovirus. Therefore, here, we report on the design and experimental validation of a sequential ceramic fluoroapatite (CFAp)–CHAp LC procedure for purification of the Sabin type 2 strain of poliovirus.

Materials and methods

Preparation of Sabin type 2 virus-containing cell culture supernatant

Vero cells (the American Type Culture Collection, Manassas, VA, USA) were cultured in minimum essential medium (MEM; Thermo Fisher Scientific Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific Inc.) and L-glutamine (2 mM; Thermo Fisher Scientific Inc.) in 225-cm2 flasks (Sumitomo Bakelite Co., Ltd., Tokyo, Japan) at 37°C in 5% CO2 for 3 days. The medium was then changed to MEM containing 2% FBS and 2 mM L-glutamine, and the cells were grown for a further day. After changing the medium to MEM (63 mL) without FBS, Sabin type 2 virus was inoculated onto the cell monolayer at a multiplicity of infection of 0.01 and cultured at 37°C for 2 days. The cell culture supernatant was then collected, passed through a 0.45-μm filter (polyethersulfone membrane; Thermo Fisher Scientific Inc.) using vacuum to remove cell debris, and stored without any stabilizer at −80°C until use. The virus was highly stable under this condition and showed no change in its TCID50 value during the 4-month storage period (data not shown).

CHAp and CFAp columns

CHT™ Ceramic Hydroxyapatite, Type II (CHAp; 40-μm particle size) and CFT™ Ceramic Fluoroapatite, Type II (CFAp; 40-μm particle size) were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). Both are ceramic-type materials with strict specifications and their pore sizes are 80–100 nm and 60–80 nm, respectively [25,26]. The particles were packed into empty stainless steel columns (4.6 mm i.d. × 35 mm; Sugiyama Shoji Co., Ltd., Kanagawa, Japan) in-house using a dry method.

Chromatographic procedures

Chromatography was performed using a BioLogic DuoFlow™ system (Bio-Rad Laboratories Inc.) with a 10- or 20-mL sample loop at a flow rate of 1.0 mL/min. The samples were loaded onto a CHAp/CFAp column and eluted using a linear gradient of sodium phosphate buffer (NaPB) ranging from 10 mM to 600 mM, details of which are provided in the legend of each figure. The resulting eluate was monitored for ultraviolet (UV) absorbance at 260 and 280 nm and for conductivity. The collected fractions were kept at 4°C and were immediately used for the evaluations described below.

Before use, the running buffers were filtered (0.22 μm). The fraction collector was placed in a biological safety cabinet, and the attached tubes were autoclaved. In addition, the interiors of the pumps, columns, and lines were sterilized with approximately 80% ethanol (Amakasu Chemical Industries, Tokyo, Japan), washed with autoclaved ultrapure water followed by 600 mM NaPB, and equilibrated with 10 mM NaPB before use. After the experiments, the column and system were sterilized with 0.5 M NaOH for 10 column volumes and washed with autoclaved ultrapure water to remove the alkaline solution.

Measurement of infectivity of the virus-containing fractions

The Sabin type 2 virus titer was obtained by measuring the median tissue culture infectious dose (TCID50) using a confluent monolayer of Vero cells in 96-well microplates. To prepare the confluent monolayer, Vero cells (100 μL, 1 × 105 cells/mL) were cultured in MEM containing 10% FBS at 37°C for 1 day. Each fraction obtained by LC separation was 10-fold serially diluted with MEM containing 10% FBS, and the resulting diluents (50 μL) were inoculated into each well (n = 3) and cultured for 1 week. Each well of the microplates was then examined under a light microscope CKX31 (Olympus Corporation, Tokyo, Japan) to determine whether cytopathic effects had occurred. Titers were calculated using the Reed–Muench method [27].

Additional procedures

The concentrations of double-stranded DNA (dsDNA) and proteins were determined using the Quant-iT™ PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific Inc.) and the Micro BCA™ Protein Assay Kit (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions. For sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), the LC fractions were concentrated using a centrifugal filter device (Ultracel YM-10; Millipore Corporation, Billerica, MA, USA) and applied to 15% polyacrylamide gel (c-PAGEL; ATTO Corporation, Tokyo, Japan). Each protein band was visualized by silver staining [28], and the molecular weight was calculated by the Gel Doc™ EZ Imager (Bio-Rad Laboratories Inc.).

Results

Two-step procedure for the purification of Sabin type 2 virus

We initially used one-step CHAp chromatography [16,17,29] to purify live attenuated Sabin type 2 virus. However, this proved unsuccessful because both protein and DNA contaminants could not be separated from the virus fraction using this method (data not shown). Therefore, we attempted to introduce separation by CFAp chromatography as the first step before conducting CHAp chromatography. The strategy of this two-step chromatographic procedure is outlined in Fig 1. In Step 1, virions were directly separated from the cell culture supernatant containing a complex protein mixture of infected cells by CFAp chromatography using pH gradient elution. In Step 2, the resulting virus fraction was separated by CHAp chromatography using NaPB gradient elution with a high concentration of sodium chloride (NaCl) to remove contaminated dsDNA from the fraction. We optimized each step as described below to separate the virus.

Fig. 1.

Standardized two-step purification procedure.
<h2>Standardized two-step purification procedure.</h2>

CFAp, ceramic fluoroapatite; CHAp, ceramic hydroxyapatite; NaPB, sodium phosphate buffer.

Step 1: pH gradient elution of Sabin type 2 virus from a CFAp column

We separated three viruses [poliovirus Sabin type 2; dengue virus type 1 (Hawaii) (Department of Virology, Institute of Tropical Medicine, Nagasaki University, Japan); and influenza virus NYMC X-181 (National Institute for Biological Standards and Control, Hertfordshire, UK)] on a CHAp column by elution with a similar gradient of NaPB at different pH values (S1–S3 Figs). We found that the retention volumes of these viruses increased as the pH of the running buffer was lowered, regardless of the physicochemical properties of the virus; however, at pH 6.4, the Sabin type 2 virus fraction still contained protein contaminants (S1 Fig), whereas the dengue virus fraction appeared to have been separated from the protein fraction (S2 Fig). Therefore, we then attempted to apply CFAp chromatography, which shows similar separation to CHAp but allows a further reduction in the pH of the running buffer due to its greater tolerance to the acidic mobile phase (applicable pH range, 5–14) compared with CHAp (applicable pH range, 6.5–14) [25,26].

We separated Sabin type 2 virus from the cell culture supernatant on a CFAp column using a linear pH gradient from pH 5.0 to 8.2 in 300 mM NaPB because use of a pH gradient reduces the contact time between the low pH and the virus (Fig 2A). The mean retention volume of the virus was found to be 9.6 ± 0.44 mL (11 independent measurements, mean ± standard deviation), indicating that the separation was highly reproducible. The resulting fraction (Fr. A indicated in Fig 2A) showed a mean recovery of 94.1% ± 42.2% (11 independent measurements) in TCID50 and a mean protein removal rate of 91.87% (two independent measurements). Analysis of the fraction using SDS–PAGE showed that there were three bands at approximately 37, 32, and 30 kDa (Fig 2B), which agreed with the previously reported separation patterns of the capsid proteins VP1, VP2, and VP3 of Sabin type 2 virus [30], indicating that the virus had been separated from the protein contaminants. Nevertheless, the fraction still contained dsDNA (mean removal rate, 88.15%; two independent measurements) derived from the host cells that needed to be removed.

Fig. 2.

Purification of Sabin type 2 virus by pH gradient elution on a ceramic fluoroapatite (CFAp) column.
<h2>Purification of Sabin type 2 virus by pH gradient elution on a ceramic fluoroapatite (CFAp) column.</h2>

(A) Chromatogram obtained under the following conditions: column, CFAp; sample (volume), cell culture supernatant containing Sabin type 2 virus (5 mL); column wash, 10 mM sodium phosphate buffer (NaPB; pH 6.4, 10 mL) and 300 mM NaPB (pH 6.4, 20 mL); equilibration, 300 mM NaPB (pH 5, 15 mL); elution, linear gradient at 300 mM NaPB from pH 5 to pH 8.2 for 10 mL; wash after separation, 300 mM NaPB (pH 8.2, 5 mL) and 600 mM NaPB (pH 8.2, 10 mL). Blue line, ultraviolet (UV) absorbance at 280 nm; black broken line, conductivity; red line, infectivity in median tissue culture infectious dose (TCID50); and purple line, double-stranded DNA (dsDNA) contents. Fr. A was pooled for the evaluation. (B) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis. The cell culture supernatant and pooled Fr. A were concentrated 10-fold and 30-fold, respectively, by ultrafiltration using a molecular weight cutoff of 10,000. The molecular weights of the marker proteins are given in kDa.

Step 2: Separation of Sabin type 2 virus from dsDNAs by CHAp: Effect of NaCl addition to the mobile phase

We next examined whether a high concentration of NaCl in the mobile phase of Step 2 would allow dsDNA to be removed from the virus fraction based on the knowledge that excess sodium ions on the phosphate site of hydroxyapatite decrease the electrostatic repulsion between the phosphate site and DNA, causing an increase in the relative affinity of the calcium site for DNA [31]. To evaluate this, we separated two viruses (influenza virus NYMC X-181 and feline calicivirus) in the presence of 0–1.5 M NaCl. We found that the retention volume of dsDNA increased with increasing NaCl concentrations in the running buffer, whereas the retention volume of the virus particles decreased (S4 and S5 Figs). Thus, a high concentration of NaCl in the mobile phase proved effective in removing dsDNA from the virus fraction.

We used 1 and 1.5 M NaCl in the mobile phase to separate Sabin type 2 virus from dsDNA in the cell culture supernatant (Fig 3). An increased retention of dsDNA compared with the virus particle was obtained in both separations, indicating that the addition of 1 M NaCl was sufficient to remove dsDNA from the virus peak (removal rate, 97.32%). Furthermore, this separation was highly reproducible, with a virus retention volume after the start of gradient elution of 5.0 ± 0.5 mL (three independent measurements).

Fig. 3.

Chromatograms of Sabin type 2 virus-containing cell culture supernatant in the presence of NaCl.
<h2>Chromatograms of Sabin type 2 virus-containing cell culture supernatant in the presence of NaCl.</h2>

Separation was carried out in the presence of (A) 1 M NaCl and (B) 1.5 M NaCl under the following conditions: column, ceramic hydroxyapatite (CHAp); sample (volume), cell culture supernatant containing Sabin type 2 virus (5 mL); buffer pH, 7.2; column wash, 10 mM sodium phosphate buffer (NaPB; 9 mL); equilibration, 10 mM NaPB with NaCl (14 mL); elution, linear gradient from 10 mM to 600 mM NaPB with NaCl for 30 mL; wash after separation, 600 mM NaPB (10 mL). Lines are the same as in Fig 2. TCID50, median tissue culture infectious dose.

Sequential two-step chromatographic purification of Sabin type 2 virus

We performed the two-step sequential procedure (Steps 1 and 2 described above) to isolate Sabin type 2 virus from the cell culture supernatant. A representative case is shown in Fig 4 and S1 Table. The virus particles were separated by pH gradient elution on a CFAp column (Fig 4A), and the resulting viral fraction “Fr. B” was pooled, relying on the average retention volume that was observed above. Fr. B was then diluted 6.7-fold with 0.9% NaCl, loaded onto a CHAp column, and eluted with a linear gradient of NaPB (pH 7.2) with 1 M NaCl (Fig 4B). The peak TCID50 was detected at 3–7 mL (Fr. C) of the retention volume and contained highly purified capsid proteins (Fig 4B and 4C).

Fig. 4.

Sequential two-step purification of Sabin type 2 virus.
<h2>Sequential two-step purification of Sabin type 2 virus.</h2>

(A) Purification of Sabin type 2 virus by pH gradient elution on a ceramic fluoroapatite (CFAp) column (Step 1). Column, CFAp; sample (volume), cell culture supernatant containing Sabin type 2 virus (10 mL); wash, 10 mM sodium phosphate buffer (NaPB) (pH 6.4, 9 mL) and 300 mM NaPB (pH 6.4, 20 mL); equilibration, 15 mL of 300 mM NaPB (pH 5); elution, linear gradient from pH 5 to pH 8.2 at 300 mM NaPB for 10 mL; wash after separation, 300 mM NaPB (pH 8.2, 5 mL) and 600 mM NaPB (pH 8.2, 10 mL). Lines are the same as in Fig 2. Fr. B was pooled and further purified in Step 2. (B) Removal of double-stranded DNA (dsDNA) from the Fr. B fraction by NaPB elution with 1 M NaCl on a ceramic hydroxyapatite (CHAp) column (Step 2). Column, CHAp; sample (volume), Fr. B obtained in (A) diluted 6.7-fold with 0.9% NaCl (17 mL); buffer, pH 7.2; column wash, 10 mM NaPB (13 mL); equilibration, 10 mM NaPB (14 mL) with 1 M NaCl; elution, linear gradient from 10 mM to 187 mM NaPB with 1 M NaCl for 10 mL; wash, 600 mM NaPB (10 mL). Fr. C was pooled for further evaluation. (C) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis of the pooled fraction obtained through the two-step purification process. The pooled Fr. C fraction obtained in (B) and the cell culture supernatant were concentrated 100- and 10-fold, respectively, by ultrafiltration using a molecular weight cutoff of 10,000. The sizes are given in kDa on the left. Arrows indicate the major proteins in Fr. C. TCID50, median tissue culture infectious dose.

Discussion

When manufacturing vaccines for use in clinical trials, preclinical studies, or commercial medicines, the aim is to minimize impurities and maximize the recovery of the vaccine during purification. The impurities are typically DNA and proteins derived from host cells, components of the cell culture medium, and/or some ligands released from purification process [32]. In this study, we successfully purified Sabin type 2 virus directly from the cell culture supernatant without using any concentrating or buffer exchange processes, achieving the mean recovery rate in TCID50 of 58.7% ± 30.0%, and the mean removal rates of proteins and dsDNA of 99.95 ± 0.006% and 99.99% ± 0.003%, respectively, (three independent experiments). The current permissible host cell protein and DNA levels in viral vaccines as recommended by the World Health Organization (WHO) are <10 μg and <10 ng per 40 D-antigen units, respectively [33]. Because our analysis was not based on the measurement of D-antigen unit-based purity, we were unable to compare between purities obtained in this study and those recommended by WHO standards. This limitation needs to be addressed when considering future scale-ups and automation. In Step 1, the cell culture supernatant was loaded directly onto a CFAp column and eluted with a pH gradient. In Step 2, the resulting virus fraction was diluted with 0.9% NaCl, loaded on a CHAp column, and eluted with an NaPB gradient in the presence of a high concentration of NaCl. Because both Steps 1 and 2 were highly reproducible, the viral fraction could be obtained without the need for any detection processes. This retention-volume-dependent fractionation should reduce the time and cost required to make vaccines. Furthermore, although the procedure currently contains multistep and offline purification processes, it should be possible to automate all the purification steps in the future. This two-step chromatographic purification is also easier to scale up than other current procedures [34] because it was constructed using fully scalable processes (Fig 5).

Fig. 5.

Tandem column system.
<h2>Tandem column system.</h2>

CFAp, ceramic fluoroapatite; CHAp, ceramic hydroxyapatite; NaPB, sodium phosphate buffer; PV, poliovirus.

Two-step chromatographic purification may also be useful for purifying other poliovirus strains, such as Salk and Sabin types 1 and 3, as well as other nonenveloped viruses, including hepatitis A virus, norovirus, and feline calicivirus. However, this process does have some methodological limitations because Step 1 uses a low pH that will disrupt some viruses. Consequently, it may not be applicable for the purification of flaviviruses, which are unstable at acidic pH values [35].

In addition to their role in vaccine production, infective virus purification techniques also play an important role in the field of gene therapy. In particular, there is an urgent clinical need for the large-scale purification of adenovirus because this virus is considered one of the most suitable platforms for long-term gene transduction [36]. Several other gene therapies have also yielded promising results for the use of virus vectors that express clinically relevant genes [37]. For example, oncolytic adenovirus has been drawing attention recently as an antitumor medicine [38], and dozens of studies using this virus are now registered in ClinicalTrials.Gov (https://clinicaltrials.gov/), a database of clinical studies conducted around the world [39]. This new purification method should be appropriate for adenoviruses that are resistant to acidic purification conditions.

Supporting information

S1 Fig [a]
Chromatograms of Sabin type 2 virus-containing cell culture supernatant separated on a ceramic hydroxyapatite (CHAp) column at different pH values.

S2 Fig [a]
Chromatograms of dengue virus type 1-containing cell culture supernatant separated on a ceramic hydroxyapatite (CHAp) column at different pH values.

S3 Fig [a]
Chromatograms of influenza virus NYMC X-181 containing cell culture supernatant separated on a ceramic hydroxyapatite (CHAp) column at different pH values.

S4 Fig [a]
Chromatograms of influenza virus NYMC X-181 containing cell culture supernatant in the presence of NaCl.

S5 Fig [a]
Chromatograms of feline calicivirus-containing cell culture supernatant in the presence of NaCl.

S1 Table [docx]
Evaluation of the obtained fractions.

S1 Appendix [docx]

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Článek "The dead shall be raised": Multidisciplinary analysis of human skeletons reveals complexity in 19th century immigrant socioeconomic history and identity in New Haven, Connecticut
Článek Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach
Článek Drink driving engagement in women: An exploration of context, hazardous alcohol use, and behaviour
Článek The effect of birth weight on body composition: Evidence from a birth cohort and a Mendelian randomization study
Článek Dolutegravir based antiretroviral therapy compared to other combined antiretroviral regimens for the treatment of HIV-infected naive patients: A systematic review and meta-analysis
Článek Expression profile of genes encoding allatoregulatory neuropeptides in females of the spider Parasteatoda tepidariorum (Araneae, Theridiidae)
Článek “My life after stroke through a camera lens”- A photovoice study on participation in Sweden
Článek Bacterial community composition of biofilms in milking machines of two dairy farms assessed by a combination of culture-dependent and –independent methods
Článek Health differences between multiple and single job holders in precarious employment in the Netherlands: A cross-sectional study among Dutch workers
Článek Clinical evaluation and budget impact analysis of cervical cancer screening using cobas 4800 HPV screening technology in the public sector of South Africa
Článek Rare earth elements in paddy fields from eroded granite hilly land in a southern China watershed
Článek High-throughput sequencing analysis of microbial community diversity in response to indica and japonica bar-transgenic rice paddy soils
Článek Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)
Článek Long-term performance of an atrial lead capable of accelerometer based detection of cardiac contractility in patients receiving cardiac resynchronisation therapy
Článek Ectomycorrhizal fungal communities of secondary tropical forests dominated by Tristaniopsis in Bangka Island, Indonesia
Článek Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice
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Článek Competing priorities and second chances - A qualitative exploration of prisoners’ journeys through the Hepatitis C continuum of care
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Článek Amyloid β oligomers inhibit growth of human cancer cells
Článek Distraction of olfactory bulb-medial prefrontal cortex circuit may induce anxiety-like behavior in allergic rhinitis
Článek Hormonal, metabolic and inflammatory circulating biomarker profiles in obese and non-obese Brazilian middle-aged women
Článek 'He usually has what we call normal fevers’: Cultural perspectives on healthy child growth in rural Southeastern Tanzania: An ethnographic enquiry
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Článek Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts
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Článek Target women: Equity in access to mHealth technology in a non-communicable disease care intervention in Kenya
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Článek Glucocorticoid stimulation increases cardiac contractility by SGK1-dependent SOCE-activation in rat cardiac myocytes
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Článek Can they imagine the future? A qualitative study exploring the skills employers seek in pharmaceutical sciences doctoral graduates
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Článek Time trends in healthy lifestyle among adults in Germany: Results from three national health interview and examination surveys between 1990 and 2011
Článek The impact of door-to-electrocardiogram time on door-to-balloon time after achieving the guideline-recommended target rate
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Článek CCR2 knockout ameliorates obesity-induced kidney injury through inhibiting oxidative stress and ER stress
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Článek Alcohol, tobacco and cannabis use are associated with job loss at follow-up: Findings from the CONSTANCES cohort
Článek Estimation of the incidence of animal rabies in Punjab, India
Článek G-protein-coupled receptor 40 agonist GW9508 potentiates glucose-stimulated insulin secretion through activation of protein kinase Cα and ε in INS-1 cells
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Článek Cognitive and social congruence in peer-assisted learning – A scoping review
Článek The wing of the ToxR winged helix-turn-helix domain is required for DNA binding and activation of toxT and ompU
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Článek Knowledge and practices regarding toxoplasmosis in housewives: A cross sectional study in a northern Mexican city
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Článek Can we predict which species win when new habitat becomes available?
Článek Wetlands are keystone habitats for jaguars in an intercontinental biodiversity hotspot
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Článek The Edinburgh Postpartum Depression Scale: Stable structure but subscale of limited value to detect anxiety
Článek EV71 3C protease induces apoptosis by cleavage of hnRNP A1 to promote apaf-1 translation
Článek Post-exercise high-sensitivity troponin T levels in patients with suspected unstable angina
Článek Unexpected sawtooth artifact in beat-to-beat pulse transit time measured from patient monitor data
Článek Development of highly sensitive and low-cost DNA agarose gel electrophoresis detection systems, and evaluation of non-mutagenic and loading dye-type DNA-staining reagents
Článek Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
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Článek Phylogeography of the dugong (Dugong dugon) based on historical samples identifies vulnerable Indian Ocean populations
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Článek Evaluation of the effectiveness and equity of the maternity protection reform in Chile from 2000 to 2015
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Článek Are screening methods useful in feature selection? An empirical study
Článek Free will beliefs are better predicted by dualism than determinism beliefs across different cultures
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Článek Acute pancreatitis risk after kidney transplantation: Propensity score matching analysis of a national cohort
Článek Understanding the effect of producers’ attitudes, perceived norms, and perceived behavioral control on intentions to use antimicrobials prudently on New York dairy farms
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Článek Graviola (Annona muricata) attenuates behavioural alterations and testicular oxidative stress induced by streptozotocin in diabetic rats
Článek Ecological conditions experienced by offspring during pregnancy and early post-natal life determine mandible size in roe deer
Článek Calculation of the contribution rate of China’s hydraulic science and technology based on a feedforward neural network
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Článek The absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice
Článek Relationship between Helicobacter pylori infection and obesity in Chinese adults: A systematic review with meta-analysis
Článek Moving model analysis on the transient pressure and slipstream caused by a metro train passing through a tunnel
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Článek Lower dormancy with rapid germination is an important strategy for seeds in an arid zone with unpredictable rainfall
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Článek Malaria vaccine candidates displayed on novel virus-like particles are immunogenic and induce transmission-blocking activity
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Článek Falls among community-dwelling older adults in Ethiopia; A preliminary cross-sectional study
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Článek The effect of ethyl alcohol on the severity of injuries in fatal pedestrian victims of traffic crashes
Článek Drought stress has transgenerational effects on soybean seed germination and seedling vigor
Článek Prevalence of benign osseous lesions of the spine and association with spinal pain in the general population in whole body MRI
Článek Age-related differences of inter-joint coordination in elderly during squat jumping
Článek Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
Článek Diagnostic performance and image quality of iterative model-based reconstruction of coronary CT angiography using 100 kVp for heavily calcified coronary vessels
Článek CAC1 knockdown reverses drug resistance through the downregulation of P-gp and MRP-1 expression in colorectal cancer
Článek Getting your game on: Using virtual reality to improve real table tennis skills
Článek Microbiological contamination of young children’s hands in rural Bangladesh: Associations with child age and observed hand cleanliness as proxy
Článek Expansion of the agricultural frontier in the largest South American Dry Forest: Identifying priority conservation areas for snakes before everything is lost
Článek Utility of lung ultrasound in ANCA-associated vasculitis with lung involvement
Článek Aerial surveys cause large but ephemeral decreases in bear presence at salmon streams in Kodiak, Alaska
Článek Exposure to particle debris generated from passenger and truck tires induces different genotoxicity and inflammatory responses in the RAW 264.7 cell line
Článek Influence of the cultivation medium and pH on the pigmentation of Trichophyton rubrum
Článek Intra-arterial catheter-directed CT angiography for assessment of endovascular aortic aneurysm repair
Článek Association of antibiotic exposure with the mortality in metastatic colorectal cancer patients treated with bevacizumab-containing chemotherapy: A hospital-based retrospective cohort study
Článek The genetic alteration spectrum of the SWI/SNF complex: The oncogenic roles of BRD9 and ACTL6A
Článek Plasma chemistry in nesting leatherback sea turtles (Dermochelys coriacea) from Florida: Understanding the importance of sample hemolysis effects on blood analytes
Článek Myotendinous asymmetries derived from the prolonged practice of badminton in professional players
Článek Computational tools to detect signatures of mutational processes in DNA from tumours: A review and empirical comparison of performance
Článek Exposure to lipopolysaccharide (LPS) reduces contractile response of small airways from GSTCD-/- mice
Článek Generation of a protective murine monoclonal antibody against the stem of influenza hemagglutinins from group 1 viruses and identification of resistance mutations against it
Článek Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations
Článek Radiation hardness of cadmium telluride solar cells in proton therapy beam mode
Článek Bourdieu, networks, and movements: Using the concepts of habitus, field and capital to understand a network analysis of gender differences in undergraduate physics
Článek A 32-channel parallel transmit system add-on for 7T MRI
Článek Comparison between Aptima Assays (Hologic) and the Allplex STI Essential Assay (Seegene) for the diagnosis of Sexually transmitted infections
Článek Knowledge gaps of STIs in Africa; Systematic review
Článek Correction: Bovine herpesvirus 1 can cross the intact zona pellucida of bovine oocytes after artificial infection
Článek Distribution of corneal spherical aberration in a Tanzanian population
Článek Correction: Determinants of physical activity: A path model based on an ecological model of active living
Článek Wild pollinator activity negatively related to honey bee colony densities in urban context
Článek Subclinical alterations in left ventricular structure and function according to obesity and metabolic health status
Článek The epidemiological signature of influenza B virus and its B/Victoria and B/Yamagata lineages in the 21st century
Článek Identification of bovine CpG SNPs as potential targets for epigenetic regulation via DNA methylation
Článek Nitric oxide regulates the expression of heme carrier protein-1 via hypoxia inducible factor-1α stabilization
Článek Development of highly efficient protocols for extraction and amplification of cytomegalovirus DNA from dried blood spots for detection and genotyping of polymorphic immunomodulatory genes
Článek Construction of pseudomolecule sequences of Brassica rapa ssp. pekinensis inbred line CT001 and analysis of spontaneous mutations derived via sexual propagation
Článek Effects of flavoring compounds used in electronic cigarette refill liquids on endothelial and vascular function
Článek Rural pipeline and willingness to work in rural areas: Mixed method study on students in midwifery and obstetric nursing in Mali
Článek Role of phospholipase A2 receptor 1 antibody level at diagnosis for long-term renal outcome in membranous nephropathy
Článek Prevalence of anemia in diabetic adult outpatients in Northeast Ethiopia
Článek Characterization of melanin and optimal conditions for pigment production by an endophytic fungus, Spissiomyces endophytica SDBR-CMU319
Článek Genetic variability and consequence of Mycobacterium tuberculosis lineage 3 in Kampala-Uganda
Článek Comparison of in vitro and computational experiments on the relation of inter-beat interval and duration of repolarization in a specific type of human induced pluripotent stem cell-derived cardiomyocytes
Článek Current and potential contributions of community pharmacy teams to self-harm and suicide prevention: A qualitative interview study
Článek Prediction of risk scores for colorectal cancer patients from the concentration of proteins involved in mitochondrial apoptotic pathway
Článek With or without U(K): A pre-Brexit network analysis of the EU ETS
Článek Graph-theoretical analysis for energy landscape reveals the organization of state transitions in the resting-state human cerebral cortex
Článek Task-uninformative visual stimuli improve auditory spatial discrimination in humans but not the ideal observer
Článek Residual stenosis after carotid artery stenting: Effect on periprocedural and long-term outcomes
Článek gapFinisher: A reliable gap filling pipeline for SSPACE-LongRead scaffolder output
Článek A plant biostimulant made from the marine brown algae Ascophyllum nodosum and chitosan reduce Fusarium head blight and mycotoxin contamination in wheat
Článek Monte-Carlo value analysis of High-Throughput Satellites: Value levers, tradeoffs, and implications for operators and investors
Článek Educational and health outcomes associated with bronchopulmonary dysplasia in 15-year-olds born preterm
Článek Loss of prostatic acid phosphatase and α-synuclein cause motor circuit degeneration without altering cerebellar patterning
Článek Neuropathy and neural plasticity in the subcutaneous white adipose depot
Článek Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells
Článek The enemy’s gaze: Immersive virtual environments enhance peace promoting attitudes and emotions in violent intergroup conflicts
Článek Protective effect of lactobacillus plantarum on alcoholic liver injury and regulating of keap-Nrf2-ARE signaling pathway in zebrafish larvae
Článek Koopman Mode Analysis of agent-based models of logistics processes
Článek Empirical mode decomposition based long short-term memory neural network forecasting model for the short-term metro passenger flow
Článek Male sexual dysfunction in obesity: The role of sex hormones and small fibre neuropathy
Článek Multi-agent reinforcement learning with approximate model learning for competitive games
Článek Identification of olfactory genes and functional analysis of BminCSP and BminOBP21 in Bactrocera minax
Článek The use of urinary fluoride excretion to facilitate monitoring fluoride intake: A systematic scoping review
Článek Development of a family caregiver needs-assessment scale for end-of-life care for senility at home (FADE)
Článek Epidemiology of pneumonia in the pre-pneumococcal conjugate vaccine era in children 2-59 months of age, in Ulaanbaatar, Mongolia, 2015-2016
Článek H-EM: An algorithm for simultaneous cell diameter and intensity quantification in low-resolution imaging cytometry
Článek Public expenditure on Non-Communicable Diseases & Injuries in India: A budget-based analysis
Článek Doxorubicin induces trans-differentiation and MMP1 expression in cardiac fibroblasts via cell death-independent pathways
Článek uORF-Tools—Workflow for the determination of translation-regulatory upstream open reading frames
Článek Comparative analysis of postural control and vertical jump performance between three different measurement devices
Článek Hierarchical multi-view aggregation network for sensor-based human activity recognition
Článek Clavien–Dindo classification for grading complications after total pharyngolaryngectomy and free jejunum transfer
Článek Significantly different expression levels of microRNAs associated with vascular invasion in hepatocellular carcinoma and their prognostic significance after surgical resection
Článek Clinical correlates of workplace injury occurrence and recurrence in adults
Článek Medicine and the media: Medical experts’ problems and solutions while working with journalists
Článek Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrhegenic E. coli  strains isolated from community acquired infections
Článek Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique
Článek Forest resilience under global environmental change: Do we have the information we need? A systematic review
Článek Improving the production of podophyllotoxin in hairy roots of Hyptis suaveolens induced from regenerated plantlets
Článek Controls on planktonic foraminifera apparent calcification depths for the northern equatorial Indian Ocean
Článek Notch and Delta are required for survival of the germline stem cell lineage in testes of Drosophila melanogaster
Článek Children with HIV: A scoping review of auditory processing skills
Článek Correction: Two distinct actin waves correlated with turns-and-runs of crawling microglia
Článek Expression of Concern: Neurotoxicity Induced by Bupivacaine via T-Type Calcium Channels in SH-SY5Y Cells
Článek Sex-dependent and -independent transcriptional changes during haploid phase gametogenesis in the sugar kelp Saccharina latissima
Článek Vehicle modeling for the analysis of the response of detectors based on inductive loops
Článek New Mycobacteroides abscessus subsp. massiliense strains with recombinant hsp65 gene laterally transferred from Mycobacteroides abscessus subsp. abscessus: Potential for misidentification of M. abscessus strains with the hsp65-based method
Článek A prognostic Bayesian network that makes personalized predictions of poor prognostic outcome post resection of pancreatic ductal adenocarcinoma
Článek Impact of care provider network characteristics on patient outcomes: Usage of social network analysis and a multi-scale community detection
Článek Valid group comparisons can be made with the Patient Health Questionnaire (PHQ-9): A measurement invariance study across groups by demographic characteristics
Článek Comparison of risk models for mortality and cardiovascular events between machine learning and conventional logistic regression analysis
Článek Upgraded molecular models of the human KCNQ1 potassium channel
Článek Mental health and quality of life outcomes in family members of patients with chronic critical illness admitted to the intensive care units of two Brazilian hospitals serving the extremes of the socioeconomic spectrum
Článek Correction: Secretome profiling of PC3/nKR cells, a novel highly migrating prostate cancer subline derived from PC3 cells
Článek The extraordinary osteology and functional morphology of the limbs in Palorchestidae, a family of strange extinct marsupial giants
Článek Primary hyperhidrosis prevalence and characteristics among medical students in Rio de Janeiro
Článek Implementation of home blood pressure monitoring among French GPs: A long and winding road
Článek In vitro and molecular chemosensitivity in human cholangiocarcinoma tissues
Článek Saiga horn user characteristics, motivations, and purchasing behaviour in Singapore
Článek The health and cost burden of antibiotic resistant and susceptible Escherichia coli bacteraemia in the English hospital setting: A national retrospective cohort study
Článek Retraction: Comprehensive Comparison of Three Different Immunosuppressive Regimens for Liver Transplant Patients with Hepatocellular Carcinoma: Steroid-Free Immunosuppression, Induction Immunosuppression and Standard Immunosuppression
Článek Can acute suicidality be predicted by Instagram data? Results from qualitative and quantitative language analyses
Článek Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi
Článek Antibacterial activity of silver nanoparticles of different particle size against Vibrio Natriegens
Článek Genetic variations associated with response to dutasteride in the treatment of male subjects with androgenetic alopecia
Článek To eat or not to eat: Reward delay impulsivity in children with loss of control eating, attention deficit / hyperactivity disorder, a double diagnosis, and healthy children
Článek Factors associated with newborn care knowledge and practices in the upper Himalayas
Článek Correction: A more physiological approach to lipid metabolism alterations in cancer: CRC-like organoids assessment
Článek Correlates of concurrent partnerships and patterns of condom use among men who have sex with men and transgender women in Peru
Článek Ocular and systemic risk factors associated with recurrent disc hemorrhage in primary open-angle glaucoma
Článek Development of a denoising convolutional neural network-based algorithm for metal artifact reduction in digital tomosynthesis for arthroplasty: A phantom study
Článek Low-level sensory processes play a more crucial role than high-level cognitive ones in the size-weight illusion
Článek The prevalence of asthma and allergic rhinitis in Nigeria: A nationwide survey among children, adolescents and adults
Článek Retraction: Human Cystathionine-β-Synthase Phosphorylation on Serine227 Modulates Hydrogen Sulfide Production in Human Urothelium
Článek Which clinical and biochemical predictors should be used to screen for diabetes in patients with serious mental illness receiving antipsychotic medication? A large observational study
Článek Characteristics of diabetic macular edema patients refractory to anti-VEGF treatments and a dexamethasone implant
Článek Correction: Effects of dietary n-6: n-3 polyunsaturated fatty acid ratios on meat quality, carcass characteristics, tissue fatty acid profiles, and expression of lipogenic genes in growing goats
Článek Speckle tracking derived reference values of myocardial deformation and impact of cardiovascular risk factors – Results from the population-based STAAB cohort study
Článek Impact of pterygium on the ocular surface and meibomian glands
Článek Lack of association between hypothyroxinemia of prematurity and transient thyroid abnormalities with adverse long term neurodevelopmental outcome in very low birth weight infants
Článek Transcriptome divergence during leaf development in two contrasting switchgrass (Panicum virgatum L.) cultivars
Článek Study on Dalfampridine in the treatment of Multiple Sclerosis Mobility Disability: A meta-analysis
Článek Circulating progenitor cells and the expression of Cxcl12, Cxcr4 and angiopoietin-like 4 during wound healing in the murine ear
Článek EUSKOR: End-to-end coreference resolution system for Basque
Článek Inactivating pathogenic bacteria in greywater by biosynthesized Cu/Zn nanoparticles from secondary metabolite of Aspergillus iizukae; optimization, mechanism and techno economic analysis
Článek Exopolysaccharide producing rhizobacteria and their impact on growth and drought tolerance of wheat grown under rainfed conditions
Článek Structural equation modeling for hypertension and type 2 diabetes based on multiple SNPs and multiple phenotypes
Článek Correction: Association between ultraviolet radiation exposure dose and cataract in Han people living in China and Taiwan: A cross-sectional study
Článek Short-term exercise training improves cardiac function associated to a better antioxidant response and lower type 3 iodothyronine deiodinase activity after myocardial infarction
Článek Unexpected low genetic variation in the South American hystricognath rodent Lagostomus maximus (Rodentia: Chinchillidae)
Článek PRC2 activates interferon-stimulated genes indirectly by repressing miRNAs in glioblastoma
Článek Surgical resection is sufficient for incidentally discovered solitary pulmonary nodule caused by nontuberculous mycobacteria in asymptomatic patients
Článek “I do all I can but I still fail them”: Health system barriers to providing Option B+ to pregnant and lactating women in Malawi
Článek Impact of major illnesses and geographic regions on do-not-resuscitate rate and its potential cost savings in Taiwan
Článek Implementation of intermittent theta burst stimulation compared to conventional repetitive transcranial magnetic stimulation in patients with treatment resistant depression: A cost analysis
Článek Genome-wide analysis of DNA methylation profile identifies differentially methylated loci associated with human intervertebral disc degeneration
Článek Correction: Artificial neural networks reveal individual differences in metacognitive monitoring of memory
Článek Combining fish and environmental PCR for diagnostics of diseased laboratory zebrafish in recirculating systems
Článek Reading skill modulates the effect of parafoveal distractors on foveal lexical decision in deaf students
Článek Association between the posterior part of the circle of Willis and the vertebral artery hypoplasia
Článek Exposure to marital conflict: Gender differences in internalizing and externalizing problems among children
Článek Ethanol locks for the prevention of catheter-related infection in patients with central venous catheter: A systematic review and meta-analysis of randomized controlled trials
Článek The effect of age and perturbation time on online control during rapid pointing
Článek Oilseed rape (Brassica napus) resistance to growth of Leptosphaeria maculans in leaves of young plants contributes to quantitative resistance in stems of adult plants
Článek Lymphocyte proliferation induced by high-affinity peptides for HLA-B*51:01 in Behçet’s uveitis
Článek Sensitization of avian pathogenic Escherichia coli to amoxicillin in vitro and in vivo in the presence of surfactin
Článek Genomic characterization of the complete terpene synthase gene family from Cannabis sativa
Článek Inhibition of gap junctional intercellular communication by an anti-migraine agent, flunarizine
Článek Levels of serum eosinophil cationic protein are associated with hookworm infection and intensity in endemic communities in Ghana
Článek Plant growth promoting rhizobacterium Stenotrophomonas maltophilia BJ01 augments endurance against N2 starvation by modulating physiology and biochemical activities of Arachis hypogea
Článek Hierarchical integrated and segregated processing in the functional brain default mode network within attention-deficit/hyperactivity disorder
Článek Rising co-payments coincide with unwanted effects on continuity of healthcare for patients with schizophrenia in the Netherlands
Článek Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system
Článek False-negative errors in next-generation sequencing contribute substantially to inconsistency of mutation databases
Článek Identification of potassium phosphite responsive miRNAs and their targets in potato
Článek Quantification of bell-shaped size selectivity in shrimp trawl fisheries using square mesh panels and a sorting cone after a Nordmøre grid
Článek Deactivation of somatosensory and visual cortices during vestibular stimulation is associated with older age and poorer balance
Článek Combined immunization with attenuated live influenza vaccine and chimeric pneumococcal recombinant protein improves the outcome of virus-bacterial infection in mice
Článek Flooding performance evaluation of alkyl aryl sulfonate in various alkaline environments
Článek An artificial intelligent diagnostic system on mobile Android terminals for cholelithiasis by lightweight convolutional neural network
Článek Enhanced detection of prion infectivity from blood by preanalytical enrichment with peptoid-conjugated beads
Článek COI metabarcoding primer choice affects richness and recovery of indicator taxa in freshwater systems
Článek Retraction: APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway
Článek The intake pattern and feed preference of layer hens selected for high or low feed conversion ratio
Článek Individualized pattern recognition for detecting mind wandering from EEG during live lectures
Článek Ontogenetic expression of thyroid hormone signaling genes: An in vitro and in vivo species comparison
Článek Post-activation potentiation effect of eccentric overload and traditional weightlifting exercise on jumping and sprinting performance in male athletes
Článek Effectiveness of different central venous catheter fixation suture techniques: An in vitro crossover study
Článek Quantitative detection of ALK fusion breakpoints in plasma cell-free DNA from patients with non-small cell lung cancer using PCR-based target sequencing with a tiling primer set and two-step mapping/alignment
Článek Nuclei deformation reveals pressure distributions in 3D cell clusters
Článek Strategies for increasing diagnostic yield of community-onset bacteraemia within the emergency department: A retrospective study
Článek Rapid evolution of Mexican H7N3 highly pathogenic avian influenza viruses in poultry
Článek Certified service dogs – A cost-effectiveness analysis appraisal
Článek Endothelial dysfunction and low-grade inflammation in the transition to renal replacement therapy
Článek Assessing the impact of a research funder’s recommendation to consider core outcome sets
Článek Validity of six consumer-level activity monitors for measuring steps in patients with chronic heart failure
Článek Detection of deceptive motions in rugby from visual motion cues
Článek The impact of antenatal care on neonatal mortality in sub-Saharan Africa: A systematic review and meta-analysis
Článek Epidemiological investigation and management of bloody diarrhea among children in India
Článek Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation
Článek Bacteria isolated from Bengal cat (Felis catus × Prionailurus bengalensis) anal sac secretions produce volatile compounds potentially associated with animal signaling
Článek Petri net–based model of the human DNA base excision repair pathway
Článek Complexation and conformation of lead ion with poly-γ-glutamic acid in soluble state
Článek The effects of sympathetic activity induced by ice water on blood flow and brachial artery flow-mediated dilatation response in healthy volunteers
Článek The Youth-Physical Activity Towards Health (Y-PATH) intervention: Results of a 24 month cluster randomised controlled trial
Článek The COPD multi-dimensional phenotype: A new classification from the STORICO Italian observational study
Článek Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data
Článek Maintaining hope after a disabling stroke: A longitudinal qualitative study of patients’ experiences, views, information needs and approaches towards making treatment decisions
Článek Cultural differences in the use of acoustic cues for musical emotion experience
Článek Effects of a rifampicin pre-treatment on linezolid pharmacokinetics
Článek High prevalence of multidrug resistant Enterobacteriaceae among residents of long term care facilities in Amsterdam, the Netherlands
Článek Infection/inflammation-associated preterm delivery within 14 days of presentation with symptoms of preterm labour: A multivariate predictive model
Článek Prognostic value of preoperative hydronephrosis in patients with bladder cancer undergoing radical cystectomy: A meta-analysis
Článek Correction: Early life predictors of midlife allostatic load: A prospective cohort study
Článek PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
Článek Epstein-Barr virus genome packaging factors accumulate in BMRF1-cores within viral replication compartments
Článek Symbiotic incompatibility between soybean and Bradyrhizobium arises from one amino acid determinant in soybean Rj2 protein
Článek Does anticoagulation needed for distally located incidental pulmonary thromboembolism in patients with active cancer?
Článek Control of human testis-specific gene expression
Článek Speaking up culture of medical students within an academic teaching hospital: Need of faculty working in patient safety
Článek Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations
Článek Understanding parental perspectives on outcomes following paediatric encephalitis: A qualitative study
Článek Results of scoping review do not support mild traumatic brain injury being associated with a high incidence of chronic cognitive impairment: Commentary on McInnes et al. 2017
Článek Native seed, soil and atmosphere respond to boreal forest topsoil (LFH) storage
Článek Development of a quantitative polymerase chain reaction assay and environmental DNA sampling methods for Giant Gartersnake (Thamnophis gigas)
Článek Genetic diversity and population structure of four Chinese rabbit breeds
Článek Knowledge, attitude and behaviors towards patients with mental illness: Results from a national Lebanese study
Článek Correction: An impact evaluation of two rounds of mass drug administration on the prevalence of active trachoma: A clustered cross sectional survey
Článek Comparative prognostic accuracy of sepsis scores for hospital mortality in adults with suspected infection in non-ICU and ICU at an academic public hospital
Článek Graph convolutional network approach applied to predict hourly bike-sharing demands considering spatial, temporal, and global effects
Článek Impact of traffic variability on geographic accessibility to 24/7 emergency healthcare for the urban poor: A GIS study in Dhaka, Bangladesh
Článek Feeling the heat: Elevated temperature affects male display activity of a lekking grassland bird
Článek Constructing a comprehensive disaster resilience index: The case of Italy
Článek Intraocular pressure according to different types of tonometry (non-contact and Goldmann applanation) in patients with different degrees of bilateral tearing
Článek Prevalent vertebral fracture is dominantly associated with spinal microstructural deterioration rather than bone mineral density in patients with type 2 diabetes mellitus
Článek Silent volumetric multi-contrast 7 Tesla MRI of ocular tumors using Zero Echo Time imaging
Článek Nonverbal synchrony in virtual reality
Článek Do speed cameras reduce road traffic collisions?
Článek A zero-shot learning approach to the development of brain-computer interfaces for image retrieval
Článek Use of non-HIV medication among people living with HIV and receiving antiretroviral treatment in Côte d’Ivoire, West Africa: A cross-sectional study
Článek DNA barcoding of southern African crustaceans reveals a mix of invasive species and potential cryptic diversity
Článek Development of an international external quality assurance program for HIV-1 incidence using the Limiting Antigen Avidity assay
Článek Correction: The mean cell volume difference (dMCV) reflects serum hypertonicity in diabetic dogs
Článek Structural characteristics of lipocalin allergens: Crystal structure of the immunogenic dog allergen Can f 6
Článek Transport oil product consumption and GHG emission reduction potential in China: An electric vehicle-based scenario analysis
Článek Ontogenetic shift in the energy allocation strategy and physiological condition of larval plaice (Pleuronectes platessa)
Článek Increased proliferation and altered cell cycle regulation in pancreatic stem cells derived from patients with congenital hyperinsulinism
Článek Diagnostic accuracy of Xpert MTB/RIF assay and non-molecular methods for the diagnosis of tuberculosis lymphadenitis
Článek Assessment of glucose-6-phosphate dehydrogenase activity using CareStart G6PD rapid diagnostic test and associated genetic variants in Plasmodium vivax malaria endemic setting in Mauritania
Článek Spatial distribution of breast cancer in Sudan 2010-2016
Článek Human-induced fire regime shifts during 19th century industrialization: A robust fire regime reconstruction using northern Polish lake sediments
Článek Enhanced effectiveness of oil dispersants in destabilizing water-in-oil emulsions
Článek Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation
Článek Iron and manganese co-limit growth of the Southern Ocean diatom Chaetoceros debilis
Článek Bilateral and unilateral load-velocity profiling in a machine-based, single-joint, lower body exercise
Článek Veterans Health Administration nurses’ training and beliefs related to care of patients with traumatic brain injury
Článek Supplementation strategies affect the feed intake and performance of grazing replacement heifers
Článek Active transcutaneous bone conduction hearing implants: Systematic review and meta-analysis
Článek Parameterization-induced uncertainties and impacts of crop management harmonization in a global gridded crop model ensemble
Článek Acidification effects on isolation of extracellular vesicles from bovine milk
Článek GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve
Článek Mining version history to predict the class instability
Článek Humpback whale song occurrence reflects ecosystem variability in feeding and migratory habitat of the northeast Pacific
Článek A phase 2 study of an oral mTORC1/mTORC2 kinase inhibitor (CC-223) for non-pancreatic neuroendocrine tumors with or without carcinoid symptoms
Článek Asprosin response in hypoglycemia is not related to hypoglycemia unawareness but rather to insulin resistance in type 1 diabetes
Článek Muscle oxygenation maintained during repeated-sprints despite inspiratory muscle loading
Článek Reconstructing systematic persistent impacts of promotional marketing with empirical nonlinear dynamics
Článek Trans-national conservation and infrastructure development in the Heart of Borneo
Článek Prediction of cardiovascular disease risk among people with severe mental illness: A cohort study
Článek Data in question: A survey of European biobank professionals on ethical, legal and societal challenges of biobank research
Článek Hypertensive APOL1 risk allele carriers demonstrate greater blood pressure reduction with angiotensin receptor blockade compared to low risk carriers
Článek Reconstructing birth in Australopithecus sediba
Článek Value of contrast-enhanced ultrasound for preoperative assessment of liver reserve function in patients with liver tumors
Článek Correction: Identifying obesity/overweight status in children and adolescents; A cross-sectional medical record review of physicians’ weight screening practice in outpatient clinics, Saudi Arabia
Článek Incidence and risk factors of loss to follow-up among HIV-infected children in an antiretroviral treatment program
Článek Changes in intracellular folate metabolism during high-dose methotrexate and Leucovorin rescue therapy in children with acute lymphoblastic leukemia
Článek Functional role and evolutionary contributions of floral gland morphoanatomy in the Paleotropical genus Acridocarpus (Malpighiaceae)
Článek On the efficiency of HIV transmission: Insights through discrete time HIV models
Článek Metabolic cost calculations of gait using musculoskeletal energy models, a comparison study
Článek Comparison of molecular profile in triple-negative inflammatory and non-inflammatory breast cancer not of mesenchymal stem-like subtype
Článek Evolutionary analysis of six chloroplast genomes from three Persea americana ecological races: Insights into sequence divergences and phylogenetic relationships
Článek Cultural transmission in a food preparation task: The role of interactivity, innovation and storytelling
Článek Correction: Effects of affective priming through music on the use of emotion words
Článek Safety and immunogenicity of investigational seasonal influenza hemagglutinin DNA vaccine followed by trivalent inactivated vaccine administered intradermally or intramuscularly in healthy adults: An open-label randomized phase 1 clinical trial
Článek Estimation of vaccination coverage from electronic healthcare records; methods performance evaluation – A contribution of the ADVANCE-project
Článek Dietary phytogenics and galactomannan oligosaccharides in low fish meal and fish oil-based diets for European sea bass (Dicentrarchus labrax) juveniles: Effects on gut health and implications on in vivo gut bacterial translocation
Článek Suicide by hanging: Results from a national survey in Switzerland and its implications for suicide prevention
Článek Gene dysregulation in peripheral blood of moyamoya disease and comparison with other vascular disorders
Článek Genome wide genetic dissection of wheat quality and yield related traits and their relationship with grain shape and size traits in an elite × non-adapted bread wheat cross
Článek Safety and tolerability of artesunate-amodiaquine, artemether-lumefantrine and quinine plus clindamycin in the treatment of uncomplicated Plasmodium falciparum malaria in Kinshasa, the Democratic Republic of the Congo
Článek Factors influencing the admission decision for Medical Psychiatry Units: A concept mapping approach
Článek Comparison of post-traumatic changes in circulating and bone marrow leukocytes between BALB/c and CD-1 mouse strains
Článek Physiological stress reactivity and recovery related to behavioral traits in dogs (Canis familiaris)
Článek Associations between birth order with mental wellbeing and psychological distress in midlife: Findings from the 1970 British Cohort Study (BCS70)
Článek Non-steroidal anti-inflammatory drugs use in older adults decreases risk of Alzheimer’s disease mortality
Článek The cost-effectiveness of neonatal versus prenatal screening for congenital toxoplasmosis
Článek Ancient technology and punctuated change: Detecting the emergence of the Edomite Kingdom in the Southern Levant
Článek Flowers as viral hot spots: Honey bees (Apis mellifera) unevenly deposit viruses across plant species
Článek Randomized control trial of Tools of the Mind: Marked benefits to kindergarten children and their teachers
Článek Preserving cultural heritage: Analyzing the antifungal potential of ionic liquids tested in paper restoration
Článek PNPLA3 rs738409 G allele carriers with genotype 1b HCV cirrhosis have lower viral load but develop liver failure at younger age
Článek Correction: Unraveling the genetic complexity underlying sorghum response to water availability
Článek Impact of tear metrics on the reliability of perimetry in patients with dry eye
Článek Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
Článek Correction: Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge
Článek Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice
Článek Patient factors affecting successful linkage to treatment in a cervical cancer prevention program in Kenya: A prospective cohort study
Článek Is this a man’s world? The effect of gender diversity and gender equality on firm innovativeness
Článek Procalcitonin to stop antibiotics after cardiovascular surgery in a pediatric intensive care unit—The PROSACAB study
Článek Temporal trends, predictors, and outcomes of acute kidney injury and hemodialysis use in acute myocardial infarction-related cardiogenic shock
Článek Quantitation of free glycation compounds in saliva
Článek Resolving an 87-year-old taxonomical curiosity with the description of Psylla frodobagginsi sp. nov. (Hemiptera: Sternorrhyncha: Psyllidae), a second distinct Psylla species on the New Zealand endemic plant kōwhai
Článek The effect of cathodal tDCS on fear extinction: A cross-measures study
Článek Physiological responses to affiliation during conversation: Comparing neurotypical males and males with Asperger syndrome
Článek Human cord blood (hCB)-CD34+ humanized mice fail to reject human acute myeloid leukemia cells
Článek Investigating the dispersal of antibiotic resistance associated genes from manure application to soil and drainage waters in simulated agricultural farmland systems
Článek Continuous norming of psychometric tests: A simulation study of parametric and semi-parametric approaches
Článek Phylogenetic microbiota profiling in fecal samples depends on combination of sequencing depth and choice of NGS analysis method
Článek Cost-effectiveness of apixaban compared to other anticoagulants in patients with atrial fibrillation in the real-world and trial settings
Článek Biochemical profile and in vitro biological activities of extracts from seven folk medicinal plants growing wild in southern Tunisia
Článek Low genetic differentiation yet high phenotypic variation in the invasive populations of Spartina alterniflora in Guangxi, China
Článek A mathematical model for designing networks of C-Reactive Protein point of care testing
Článek Prevalence of hypochondriac symptoms among health science students in China: A systematic review and meta-analysis
Článek Femtosecond laser induced step-like structures inside transparent hydrogel due to laser induced threshold reduction
Článek From In Situ to satellite observations of pelagic Sargassum distribution and aggregation in the Tropical North Atlantic Ocean
Článek The polyether ionophore salinomycin targets multiple cellular pathways to block proliferative vitreoretinopathy pathology
Článek BRAF V600E and Pten deletion in mice produces a histiocytic disorder with features of Langerhans cell histiocytosis
Článek HIV prevalence and risk behavior among male and female adults screened for enrolment into a vaccine preparedness study in Maputo, Mozambique
Článek Sputum microbiota and inflammation at stable state and during exacerbations in a cohort of chronic obstructive pulmonary disease (COPD) patients
Článek Direct estimation of the parameters of a delayed, intermittent activation feedback model of postural sway during quiet standing
Článek Occurrence mechanism and coping paths of accidents of highly aggregated tourist crowds based on system dynamics
Článek Correction: Low Dose Aerosol Fitness at the Innate Phase of Murine Infection Better Predicts Virulence amongst Clinical Strains of Mycobacterium tuberculosis
Článek Effects of enalapril and paricalcitol treatment on diabetic nephropathy and renal expressions of TNF-α, p53, caspase-3 and Bcl-2 in STZ-induced diabetic rats
Článek Correction: Public reaction to Chikungunya outbreaks in Italy—Insights from an extensive novel data streams-based structural equation modeling analysis
Článek Correction: Comparison of neurodegenerative types using different brain MRI analysis metrics in older adults with normal cognition, mild cognitive impairment, and Alzheimer’s dementia
Článek Intra-individual variability of sleep and nocturnal cardiac autonomic activity in elite female soccer players during an international tournament
Článek Therapeutic efficacy of equine botulism heptavalent antitoxin against all seven botulinum neurotoxins in symptomatic guinea pigs
Článek Interleukin 10 knock-down in bovine monocyte-derived macrophages has distinct effects during infection with two divergent strains of Mycobacterium bovis
Článek Clinical outcomes of bortezomib-based therapy in Taiwanese patients with multiple myeloma: A nationwide population-based study and a single-institute analysis
Článek Scope and efficacy of the broad-spectrum topical antiseptic choline geranate
Článek Hematological and biochemical parameters for Chinese rhesus macaque
Článek Veterinary peer study groups as a method of continuous education—A new approach to identify and address factors associated with antimicrobial prescribing
Článek Correction: The prognosis of heart failure patients: Does sodium level play a significant role?
Článek Maternal malaria but not schistosomiasis is associated with a higher risk of febrile infection in infant during the first 3 months of life: A mother-child cohort in Benin
Článek Systematic identification of facility-based stillbirths and neonatal deaths through the piloted use of an adapted RAPID tool in Liberia and Nepal
Článek Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis
Článek Correction: Age, sex and storage time influence hair cortisol levels in a wild mammal population
Článek Reasons to care: Personal motivation as a key factor in the practice of the professional foster carer in Romania
Článek Determinants of clinical, functional and personal recovery for people with schizophrenia and other severe mental illnesses: A cross-sectional analysis
Článek Continuity of care for TB patients at a South African hospital: A qualitative participatory study of the experiences of hospital staff
Článek EGF receptor stimulation shifts breast cancer cell glucose metabolism toward glycolytic flux through PI3 kinase signaling
Článek The Better Management of Patients with Osteoarthritis Program: Outcomes after evidence-based education and exercise delivered nationwide in Sweden
Článek The negative effects of short-term extreme thermal events on the seagrass Posidonia oceanica are exacerbated by ammonium additions
Článek Pathogen invasion history elucidates contemporary host pathogen dynamics
Článek Chinese herbal formulae for the treatment of menopausal hot flushes: A systematic review and meta-analysis
Článek Healthcare resource utilization and costs for multiple sclerosis management in the Campania region of Italy: Comparison between centre-based and local service healthcare delivery
Článek Deconstruction of central line insertion guidelines based on the positive deviance approach—Reducing gaps between guidelines and implementation: A qualitative ethnographic research
Článek PBMCs transcriptome profiles identified breed-specific transcriptome signatures for PRRSV vaccination in German Landrace and Pietrain pigs
Článek The impact of admission serum lactate on children with moderate to severe traumatic brain injury
Článek Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface
Článek Determination of the bruise degree for cherry using Vis-NIR reflection spectroscopy coupled with multivariate analysis
Článek Genome-wide identification and expression profile analysis of nuclear factor Y family genes in Sorghum bicolor L. (Moench)
Článek Trends in maternal prepregnancy body mass index (BMI) and its association with birth and maternal outcomes in California, 2007–2016: A retrospective cohort study
Článek Distribution of the Duffy genotypes in Malaysian Borneo and its relation to Plasmodium knowlesi malaria susceptibility
Článek Hippocampal connectivity with sensorimotor cortex during volitional finger movements: Laterality and relationship to motor learning
Článek A self-adaptive deep learning method for automated eye laterality detection based on color fundus photography
Článek The effect of child marriage on the utilization of maternal health care in Nepal: A cross-sectional analysis of Demographic and Health Survey 2016
Článek Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)
Článek Chemical volatiles present in cotton gin trash: A by-product of cotton processing
Článek PTP1B negatively regulates nitric oxide-mediated Pseudomonas aeruginosa killing by neutrophils
Článek Differential metabolomics networks analysis of menopausal status
Článek Experimental study of the temporal profile of breath alcohol concentration in a Chinese population after a light meal
Článek Association between regional brain volumes and BMI z-score change over one year in children
Článek Relationships between Potentially Toxic Elements in intertidal sediments and their bioaccumulation by benthic invertebrates
Článek Long-term outcomes of dialysis in patients with chronic kidney disease and new-onset atrial fibrillation: A population-based cohort study
Článek Hospital burden of pulmonary arterial hypertension in France
Článek Understanding the variability of Australian fire weather between 1973 and 2017
Článek Photon-counting cine-cardiac CT in the mouse
Článek Ecophysiological impacts of Esca, a devastating grapevine trunk disease, on Vitis vinifera L.
Článek Black “Reading the Mind in the Eyes” task: The development of a task assessing mentalizing from black faces
Článek Osmolytes ameliorate the effects of stress in the absence of the heat shock protein Hsp104 in Saccharomyces cerevisiae
Článek Reliable and robust method for abdominal muscle mass quantification using CT/MRI: An explorative study in healthy subjects
Článek Validation of the Rainbow Model of Integrated Care Measurement Tools (RMIC-MTs) in renal care for patient and care providers
Článek Multiple origins and the population genetic structure of Rubus takesimensis (Rosaceae) on Ulleung Island: Implications for the genetic consequences of anagenetic speciation
Článek Genetic profiling of fatty acid desaturase polymorphisms identifies patients who may benefit from high-dose omega-3 fatty acids in cardiac remodeling after acute myocardial infarction—Post-hoc analysis from the OMEGA-REMODEL randomized controlled trial
Článek A randomized controlled trial examining the efficacy of an internet-based cognitive behavioral therapy program for adolescents with anxiety disorders
Článek Optimising outputs from a validated online instrument to measure health-related quality of life (HRQL) in dogs
Článek Young adults’ perceptions of using wearables, social media and other technologies to detect worsening mental health: A qualitative study
Článek Characteristics of prescription in 29 Level 3 Neonatal Wards over a 2-year period (2017-2018). An inventory for future research
Článek Genetic characterization of fall armyworm (Spodoptera frugiperda) in Ecuador and comparisons with regional populations identify likely migratory relationships
Článek Correction: Lkb1 Deficiency Alters Goblet and Paneth Cell Differentiation in the Small Intestine
Článek Cardiac resynchronization therapy-heart failure (CRT-HF) clinic: A novel model of care
Článek Synteny and phylogenetic analysis of paralogous thyrostimulin beta subunits (GpB5) in vertebrates
Článek Dual-Subpopulation as reciprocal optional external archives for differential evolution
Článek NGS analysis in Marfan syndrome spectrum: Combination of rare and common genetic variants to improve genotype-phenotype correlation analysis
Článek Optimization of cataract surgery follow-up: A standard set of questions can predict unexpected management changes at postoperative week one
Článek Silymarin in non-cirrhotics with non-alcoholic steatohepatitis: A randomized, double-blind, placebo controlled trial
Článek Clinical evaluation of General Electric new Swiftscan solution in bone scintigraphy on NaI-camera: A head to head comparison with Siemens Symbia
Článek Pharmacist-led academic detailing improves statin therapy prescribing for Malaysian patients with type 2 diabetes: Quasi-experimental design
Článek Proton pump inhibitors attenuate myofibroblast formation associated with thyroid eye disease through the aryl hydrocarbon receptor
Článek Clinical outcomes with neoadjuvant versus adjuvant chemotherapy for triple negative breast cancer: A report from the National Cancer Database
Článek Identification and characterization of a novel heparan sulfate-binding domain in Activin A longest variants and implications for function
Článek Wikipedia network analysis of cancer interactions and world influence
Článek Using path signatures to predict a diagnosis of Alzheimer’s disease
Článek Development and verification of prediction models for preventing cardiovascular diseases
Článek Additive and heterozygous (dis)advantage GWAS models reveal candidate genes involved in the genotypic variation of maize hybrids to Azospirillum brasilense
Článek Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis
Článek Transience effect in capture-recapture studies: The importance of its biological meaning
Článek Detailed global modelling of soil organic carbon in cropland, grassland and forest soils
Článek Oral dosing for antenatal corticosteroids in the Rhesus macaque
Článek Retraction: Modulation of the Pentose Phosphate Pathway Induces Endodermal Differentiation in Embryonic Stem Cells
Článek An analysis on HBsAg, Anti-HCV, Anti-HIV½ and VDRL test results in blood donors according to gender, age range and years
Článek Clinical factors associated with bacterial translocation in Japanese patients with type 2 diabetes: A retrospective study
Článek Meaningful work and resilience among teachers: The mediating role of work engagement and job crafting
Článek The conditional Fama-French model and endogenous illiquidity: A robust instrumental variables test
Článek Breast cancers utilize hypoxic glycogen stores via PYGB, the brain isoform of glycogen phosphorylase, to promote metastatic phenotypes
Článek Characterization of sequentially-staged cancer cells using electrorotation
Článek Vancomycin-laden calcium phosphate-calcium sulfate composite allows bone formation in a rat infection model
Článek Genetic evidence for plural introduction pathways of the invasive weed Paterson’s curse (Echium plantagineum L.) to southern Australia
Článek Risk of major autoimmune diseases in female breast cancer patients: A nationwide, population-based cohort study
Článek Real-time three-dimensional MRI for the assessment of dynamic carpal instability
Článek Interim report on the effective intraperitoneal therapy of insulin-dependent diabetes mellitus in pet dogs using “Neo-Islets,” aggregates of adipose stem and pancreatic islet cells (INAD 012-776)
Článek The frequency of bowel and bladder problems in multiple sclerosis and its relation to fatigue: A single centre experience
Článek Retraction: Analysis of Mycobacterium tuberculosis-Specific CD8 T-Cells in Patients with Active Tuberculosis and in Individuals with Latent Infection
Článek The association between role model presence and self-regulation in early adolescence: A cross-sectional study
Článek Correction: A controlled-release oral opioid supports S. aureus survival in injection drug preparation equipment and may increase bacteremia and endocarditis risk
Článek Methamphetamine regulation of activity and topology of ventral midbrain networks
Článek Evaluation and treatment of latent tuberculosis infection among healthcare workers in Korea: A multicentre cohort analysis
Článek Occult periprosthetic femoral fractures occur frequently during a long, trapezoidal, double-tapered cementless femoral stem fixation in primary THA
Článek TCF4 induces enzalutamide resistance via neuroendocrine differentiation in prostate cancer
Článek Structural characterization of EGFR exon 19 deletion mutation using molecular dynamics simulation
Článek Comparing infiltration rates in soils managed with conventional and alternative farming methods: A meta-analysis
Článek Correction: Permissivity of Primary Human Hepatocytes and Different Hepatoma Cell Lines to Cell Culture Adapted Hepatitis C Virus
Článek Radiomics features of the primary tumor fail to improve prediction of overall survival in large cohorts of CT- and PET-imaged head and neck cancer patients
Článek The complete mitochondrial genome of Calyptogena marissinica (Heterodonta: Veneroida: Vesicomyidae): Insight into the deep-sea adaptive evolution of vesicomyids
Článek Correction: In vitro larval rearing protocol for the stingless bee species Melipona scutellaris for toxicological studies
Článek Correction: Morphological and molecular identification of the dioecious “African species Volvox rousseletii (Chlorophyceae) in the water column of a Japanese lake based on field-collected and cultured materials
Článek Upper versus lower airway microbiome and metagenome in children with cystic fibrosis and their correlation with lung inflammation
Článek “…or else I close my ears” How women with obesity want to be approached and treated regarding gestational weight management: A qualitative interview study
Článek Does a spinal implant alter dual energy X-ray absorptiometry body composition measurements?
Článek Economic and livestock health impacts of birds on dairies: Evidence from a survey of Washington dairy operators
Článek Repair of subtotal tympanic membrane perforations: A temporal bone study of several tympanoplasty materials
Článek Shifts in temperature influence how Batrachochytrium dendrobatidis infects amphibian larvae
Článek Lung clearance index to detect the efficacy of Aztreonam lysine inhalation in patients with cystic fibrosis and near normal spirometry – A single-centre feasibility study
Článek Frequency and distribution of corneal astigmatism and keratometry features in adult life: Methodology and findings of the UK Biobank study
Článek Differences in the impedance of cochlear implant devices within 24 hours of their implantation
Článek Suppressive impact of metronomic chemotherapy using UFT and/or cyclophosphamide on mediators of breast cancer dissemination and invasion
Článek Expression of Concern: miRNA 17 Family Regulates Cisplatin-Resistant and Metastasis by Targeting TGFbetaR2 in NSCLC
Článek Group leaders establish cooperative norms that persist in subsequent interactions
Článek Addis Ababa population-based pattern of cancer therapy, Ethiopia
Článek Agreement between the Cochrane risk of bias tool and Physiotherapy Evidence Database (PEDro) scale: A meta-epidemiological study of randomized controlled trials of physical therapy interventions
Článek Oxidoreductase disulfide bond proteins DsbA and DsbB form an active redox pair in Chlamydia trachomatis, a bacterium with disulfide dependent infection and development
Článek DNA barcoding of coastal ray-finned fishes in Vietnam
Článek Comparison of climbing-specific strength and endurance between lead and boulder climbers
Článek The performance of different case definitions for severe influenza surveillance among HIV-infected and HIV-uninfected children aged <5 years in South Africa, 2011–2015
Článek Analgesic drug use in elderly persons: A population-based study in Southern Italy
Článek RCER: Reliable Cluster-based Energy-aware Routing protocol for heterogeneous Wireless Sensor Networks
Článek Beta-defensins and analogs in Helicobacter pylori infections: mRNA expression levels, DNA methylation, and antibacterial activity
Článek Changing educational gradient in long-term care-free life expectancy among German men, 1997-2012
Článek A non-parametric significance test to compare corpora
Článek Evaluating the impact of policies recommending PrEP to subpopulations of men and transgender women who have sex with men based on demographic and behavioral risk factors
Článek Different levels of statistical learning - Hidden potentials of sequence learning tasks
Článek Dry period heat stress induces microstructural changes in the lactating mammary gland
Článek Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter
Článek High levels of fasting glucose and glycosylated hemoglobin values are associated with hyperfiltration in a Spanish prediabetes cohort. The PREDAPS Study
Článek Genetically distinct Group B Streptococcus strains induce varying macrophage cytokine responses
Článek Dynamic up- and down-regulation of the default (DMN) and extrinsic (EMN) mode networks during alternating task-on and task-off periods
Článek ERK1/ATF-2 signaling axis contributes to interleukin-1β-induced MMP-3 expression in dermal fibroblasts
Článek Cytokine profiles of umbilical cord blood mononuclear cells upon in vitro stimulation with lipopolysaccharides of different vaginal gram-negative bacteria
Článek Everolimus in de novo kidney transplant recipients participating in the Eurotransplant senior program: Results of a prospective randomized multicenter study (SENATOR)
Článek Lipopolysaccharide induces mouse translocator protein (18 kDa) expression via the AP-1 complex in the microglial cell line, BV-2
Článek Does effectiveness in performance appraisal improve with rater training?
Článek National trends in inpatient endometriosis admissions: Patients, procedures and outcomes, 2006−2015
Článek Affective and enjoyment responses to 12 weeks of high intensity interval training and moderate continuous training in adults with Crohn’s disease
Článek Thrombophilic risk factors in hemodialysis: Association with early vascular access occlusion and patient survival in long-term follow-up
Článek Cabbage stem flea beetle’s (Psylliodes chrysocephala L.) susceptibility to pyrethroids and tolerance to thiacloprid in the Czech Republic
Článek Implicit learning of artificial grammatical structures after inferior frontal cortex lesions
Článek Potential survival benefits from optimized chemotherapy implementation in advanced ovarian cancer: Projections from a microsimulation model
Článek Predictors of SLE relapse in pregnancy and post-partum among multi-ethnic patients in Malaysia
Článek Heat stress responses in a large set of winter wheat cultivars (Triticum aestivum L.) depend on the timing and duration of stress
Článek Mice deficient in NKLAM have attenuated inflammatory cytokine production in a Sendai virus pneumonia model
Článek Dysregulation of microRNAs and target genes networks in human abdominal aortic aneurysm tissues
Článek Quality of life and associated factors among patients with breast cancer under chemotherapy at Tikur Anbessa specialized hospital, Addis Ababa, Ethiopia
Článek Evaluation of suitable reference genes in Brassica juncea and its wild relative Camelina sativa for qRT-PCR analysis under various stress conditions
Článek Time-related immunomodulation by stressors and corticosterone transdermal application in toads
Článek Machine learning discovery of longitudinal patterns of depression and suicidal ideation
Článek Multi-scale patterns of tick occupancy and abundance across an agricultural landscape in southern Africa
Článek A matter of taste: Spatial and ontogenetic variations on the trophic ecology of the tiger shark at the Galapagos Marine Reserve
Článek Retraction: Adaptive double threshold energy detection based on Markov model for cognitive radio
Článek Adrenal gland size in obstructive sleep apnea: Morphological assessment of hypothalamic pituitary adrenal axis activity
Článek Out-of-pocket health spending among Medicare beneficiaries: Which chronic diseases are most costly?
Článek A large-scale chromosomal inversion is not associated with life history development in rainbow trout from Southeast Alaska
Článek Deployment, suicide, and overdose among comorbidity phenotypes following mild traumatic brain injury: A retrospective cohort study from the Chronic Effects of Neurotrauma Consortium
Článek Association between alcohol intake and measures of incident CKD: An analysis of nationwide health screening data
Článek Exploration of icariin analog structure space reveals key features driving potent inhibition of human phosphodiesterase-5
Článek A quantitative approach for the analysis of clinician recognition of acute respiratory distress syndrome using electronic health record data
Článek Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus
Článek Recidivism rates in individuals receiving community sentences: A systematic review
Článek The association of intensive care with utilization and costs of outpatient healthcare services and quality of life
Článek How well do cancer survivor self-classifications of anxiety, depression and stress agree with a standardised tool? Results of a cross-sectional study
Článek Comparative nutritional characteristics of the three major Chinese Dendrobium species with different growth years
Článek Nilotinib, an approved leukemia drug, inhibits smoothened signaling in Hedgehog-dependent medulloblastoma
Článek Geographic differentiation of agritourism activities in Poland vs. cultural and natural attractiveness of destinations at district level
Článek Social participation reduces isolation among Japanese older people in urban area: A 3-year longitudinal study
Článek Explaining age-related decline in theory of mind: Evidence for intact competence but compromised executive function
Článek Role of GDF15 in methylseleninic acid-mediated inhibition of cell proliferation and induction of apoptosis in prostate cancer cells
Článek Black people are convicted more for being black than for being poor: The role of social norms and cultural prejudice on biased racial judgments
Článek Bulked segregant analysis RNA-seq (BSR-Seq) validated a stem resistance locus in Aegilops umbellulata, a wild relative of wheat
Článek Neurobehavioral dysfunction in non-alcoholic steatohepatitis is associated with hyperammonemia, gut dysbiosis, and metabolic and functional brain regional deficits
Článek Barriers and enablers to the implementation of a complex quality improvement intervention for acute kidney injury: A qualitative evaluation of stakeholder perceptions of the Tackling AKI study
Článek Soil and vegetation conditions changes following the different sand dune restoration measures on the Zoige Plateau
Článek Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections
Článek Emission characteristics of diethylhexyl phthalate (DEHP) from building materials determined using a passive flux sampler and micro-chamber
Článek Multi-focus microscope with HiLo algorithm for fast 3-D fluorescent imaging
Článek Pontoscolex corethrurus: A homeless invasive tropical earthworm?
Článek Modeling reciprocal effects in medical research: Critical discussion on the current practices and potential alternative models
Článek Does palliative chemotherapy really palliate and are we measuring it correctly? A mixed methods longitudinal study of health related quality of life in advanced soft tissue sarcoma
Článek Developing cookies formulated with goat cream enriched with conjugated linoleic acid
Článek The effects of combining focus of attention and autonomy support on shot accuracy in the penalty kick
Článek Association between hot flashes severity and oxidative stress among Mexican postmenopausal women: A cross-sectional study
Článek A combined strategy of neuropeptide prediction and tandem mass spectrometry identifies evolutionarily conserved ancient neuropeptides in the sea anemone Nematostella vectensis
Článek Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
Článek Antitumor activity of a novel dual functional podophyllotoxin derivative involved PI3K/AKT/mTOR pathway
Článek Genome-wide developed microsatellites reveal a weak population differentiation in the hoverfly Eupeodes corollae (Diptera: Syrphidae) across China
Článek Prognosis of severe acquired brain injury: Short and long-term outcome determinants and their potential clinical relevance after rehabilitation. A comprehensive approach to analyze cohort studies
Článek An in silico investigation of menthol metabolism
Článek Variant analysis pipeline for accurate detection of genomic variants from transcriptome sequencing data
Článek Identification of candidate flowering and sex genes in white Guinea yam (D. rotundata Poir.) by SuperSAGE transcriptome profiling
Článek Optimizing the intrinsic parallel diffusivity in NODDI: An extensive empirical evaluation
Článek The quality of guidelines for diabetic foot ulcers: A critical appraisal using the AGREE II instrument
Článek Mastitis risk effect on the economic consequences of paratuberculosis control in dairy cattle: A stochastic modeling study
Článek Confounding by indication of the safety of de-escalation in community-acquired pneumonia: A simulation study embedded in a prospective cohort
Článek Effects from diet-induced gut microbiota dysbiosis and obesity can be ameliorated by fecal microbiota transplantation: A multiomics approach
Článek Drivers of HIV-1 transmission: The Portuguese case
Článek Integration of a FT expression cassette into CRISPR/Cas9 construct enables fast generation and easy identification of transgene-free mutants in Arabidopsis
Článek A biface production older than 600 ka ago at Notarchirico (Southern Italy) contribution to understanding early Acheulean cognition and skills in Europe
Článek Mass evacuation and increases in long-term care benefits: Lessons from the Fukushima nuclear disaster
Článek Circulating levels of free 25(OH)D increase at the onset of rheumatoid arthritis
Článek The expression of equine keratins K42 and K124 is restricted to the hoof epidermal lamellae of Equus caballus
Článek Soil respiration from fields under three crop rotation treatments and three straw retention treatments
Článek Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification
Článek Linkage disequilibrium and haplotype block patterns in popcorn populations
Článek Downscaling satellite soil moisture using geomorphometry and machine learning
Článek Choosing efficient actions: Deciding where to walk
Článek The zooarchaeology and isotopic ecology of the Bahamian hutia (Geocapromys ingrahami): Evidence for pre-Columbian anthropogenic management
Článek Synergy of the flow behaviour and disperse phase of cellulose nanoparticles in enhancing oil recovery at reservoir condition
Článek Accuracy of Determine TB-LAM Ag to detect TB in HIV infected patients associated with diagnostic methods used in Brazilian public health units
Článek Human papillomavirus infection among head and neck squamous cell carcinomas in southern China
Článek Comparison of feature point detectors for multimodal image registration in plant phenotyping
Článek Quartet-based inference of cell differentiation trees from ChIP-Seq histone modification data
Článek Detection and prognostic relevance of circulating tumour cells (CTCs) in Asian breast cancers using a label-free microfluidic platform
Článek Coralline algal calcification: A morphological and process-based understanding
Článek Comparing record linkage software programs and algorithms using real-world data
Článek Antimicrobial resistance and molecular genotyping of Salmonella enterica serovar Enteritidis clinical isolates from Guizhou province of Southwestern China
Článek Measuring sexual relationship power equity among young women and young men South Africa: Implications for gender-transformative programming
Článek Effectiveness, safety/tolerability of OBV/PTV/r ± DSV in patients with HCV genotype 1 or 4 with/without HIV-1 co-infection, chronic kidney disease (CKD) stage IIIb-V and dialysis in Spanish clinical practice – Vie-KinD study
Článek The genome of Alcaligenes aquatilis strain BU33N: Insights into hydrocarbon degradation capacity
Článek A randomized pilot efficacy and safety trial of diazoxide choline controlled-release in patients with Prader-Willi syndrome
Článek LMX1A inhibits C-Myc expression through ANGPTL4 to exert tumor suppressive role in gastric cancer
Článek Association of vitamin D nutrition with neuro-developmental outcome of infants of slums in Bangladesh
Článek Effect of PEPFAR funding policy change on HIV service delivery in a large HIV care and treatment network in Nigeria
Článek Friends, relatives, sanity, and health: The costs of politics
Článek European public perceptions of homelessness: A knowledge, attitudes and practices survey
Článek An overview of the quality assurance programme for HIV rapid testing in South Africa: Outcome of a 2-year phased implementation of quality assurance program
Článek Transcatheter aortic valve implantation versus surgical aortic valve replacement in patients at low and intermediate risk: A risk specific meta-analysis of randomized controlled trials
Článek Global smoking trends in inflammatory bowel disease: A systematic review of inception cohorts
Článek Identification of inflammatory markers suitable for non-invasive, repeated measurement studies in biobehavioral research: A feasibility study
Článek Genomic analyses reveal an absence of contemporary introgressive admixture between fin whales and blue whales, despite known hybrids
Článek Tipping the balance towards long-term retention in the HIV care cascade: A mixed methods study in southern Mozambique
Článek Diagnosis and classification of pediatric acute appendicitis by artificial intelligence methods: An investigator-independent approach
Článek Stylistic variation on the Donald Trump Twitter account: A linguistic analysis of tweets posted between 2009 and 2018
Článek Kinematic determinants of scoring success in the fencing flick: Logistic and linear multiple regression analysis
Článek Preferences for formal and traditional sources of childbirth and postnatal care among women in rural Africa: A systematic review
Článek Spectral characteristics of urine specimens from healthy human volunteers analyzed using Raman chemometric urinalysis (Rametrix)
Článek Associations between industry involvement and study characteristics at the time of trial registration in biomedical research
Článek A review of the elusive bicolored iris Snouted Treefrogs (Anura: Hylidae:Scinax uruguayus group)
Článek Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1
Článek The predictive performance of SAPS 2 and SAPS 3 in an intermediate care unit for internal medicine at a German university transplant center; A retrospective analysis
Článek Advancing computational biology and bioinformatics research through open innovation competitions
Článek Dynamic changes in the physicochemical properties of fresh-cut produce wash water as impacted by commodity type and processing conditions
Článek Mapping online hate: A scientometric analysis on research trends and hotspots in research on online hate
Článek Orthogonal projection to latent structures and first derivative for manipulation of PLSR and SVR chemometric models' prediction: A case study
Článek DNA vaccine based on conserved HA-peptides induces strong immune response and rapidly clears influenza virus infection from vaccinated pigs
Článek Malaria transmission through the mosquito requires the function of the OMD protein
Článek Small molecule inhibition of lysine-specific demethylase 1 (LSD1) and histone deacetylase (HDAC) alone and in combination in Ewing sarcoma cell lines
Článek Governing Tripolye: Integrative architecture in Tripolye settlements
Článek In vitro selective cytotoxicity of the dietary chalcone cardamonin (CD) on melanoma compared to healthy cells is mediated by apoptosis
Článek Mortality, perception, and scale: Understanding how predation shapes space use in a wild prey population
Článek DNA analysis of elasmobranch products originating from Bangladesh reveals unregulated elasmobranch fishery and trade on species of global conservation concern
Článek Genome-wide analysis of methylation in giant pandas with cataract by methylation-dependent restriction-site associated DNA sequencing (MethylRAD)
Článek A spherical falling film gas-liquid equilibrator for rapid and continuous measurements of CO2 and other trace gases
Článek The role of vitamin D in increasing circulating T regulatory cell numbers and modulating T regulatory cell phenotypes in patients with inflammatory disease or in healthy volunteers: A systematic review
Článek Gastroenteritis and respiratory infection outbreaks in French nursing homes from 2007 to 2018: Morbidity and all-cause lethality according to the individual characteristics of residents
Článek A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction
Článek De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress
Článek Robust methods in Mendelian randomization via penalization of heterogeneous causal estimates
Článek HMGB1 mediates the development of tendinopathy due to mechanical overloading
Článek Effects of the Best Possible Self intervention: A systematic review and meta-analysis
Článek Metabolomic profiling of oxalate-degrading probiotic Lactobacillus acidophilus and Lactobacillus gasseri
Článek Adjusting cotton planting density under the climatic conditions of Henan Province, China
Článek Multiple biomarkers of sepsis identified by novel time-lapse proteomics of patient serum
Článek PTSD in prison settings: A systematic review and meta-analysis of comorbid mental disorders and problematic behaviours
Článek The female presence in different organisational positions and performance in secondary schools: Does a woman leader function as mediator?
Článek Beta power encodes contextual estimates of temporal event probability in the human brain
Článek Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
Článek Using metabolite profiling to construct and validate a metabolite risk score for predicting future weight gain
Článek Comprehensive analysis of the internal structure and firmness in American cranberry (Vaccinium macrocarpon Ait.) fruit
Článek Predicting spinal profile using 3D non-contact surface scanning: Changes in surface topography as a predictor of internal spinal alignment
Článek Assessment modelling approaches for stocks with spawning components, seasonal and spatial dynamics, and limited resources for data collection
Článek Qualitative analysis of ward staff experiences during research of a novel suicide-prevention psychological therapy for psychiatric inpatients: Understanding the barriers and facilitators
Článek De-climatizing food security: Lessons from climate change micro-simulations in Peru
Článek Influence of musculotendon geometry variability in muscle forces and hip bone-on-bone forces during walking
Článek An international survey of perceptions of the 2014 FIFA World Cup: National levels of corruption as a context for perceptions of institutional corruption
Článek Dissuasive effect, information provision, and consumer reactions to the term ‘Biotechnology’: The case of reproductive interventions in farmed fish
Článek "If I have a cancer, it is not my fault I am a refugee”: A qualitative study with expert stakeholders on cancer care management for Syrian refugees in Jordan
Článek The effect of the use of a decision aid with individual risk estimation on the mode of delivery after a caesarean section: A prospective cohort study
Článek Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale
Článek High-throughput RNA-sequencing identifies mesenchymal stem cell-induced immunological signature in a rat model of corneal allograft rejection
Článek Evaluation of the pain intensity differences among hospitalized cancer patients based on a nursing information system
Článek Effects of chitin synthesis inhibitor treatment on Lepeophtheirus salmonis (Copepoda, Caligidae) larvae
Článek Protective mechanism of 1-methylhydantoin against lung injury induced by paraquat poisoning
Článek Use of high-content analysis and machine learning to characterize complex microbial samples via morphological analysis
Článek Investigation of a spatial coupling relationship between carbon emission performance and regional urbanization in China
Článek A mutation in mouse Krüppel-like factor 15 alters the gut microbiome and response to obesogenic diet
Článek Excellent outcome after desensitization in high immunologic risk kidney transplantation
Článek Characterization of a new composite membrane for point of need paper-based micro-scale microbial fuel cell analytical devices
Článek Acute kidney injury during an ultra-distance race
Článek A mitochondria-targeted fatty acid analogue influences hepatic glucose metabolism and reduces the plasma insulin/glucose ratio in male Wistar rats
Článek The role of C-reactive protein levels on the association of physical activity with lung function in adults
Článek The CrowdWater game: A playful way to improve the accuracy of crowdsourced water level class data
Článek Therapeutic efficacy of neuregulin 1-expressing human adipose-derived mesenchymal stem cells for ischemic stroke
Článek CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein
Článek Exploring the microbiota of upper respiratory tract during the development of pneumonia in a mouse model
Článek Improved ICU mortality prediction based on SOFA scores and gastrointestinal parameters
Článek Social information use and collective foraging in a pursuit diving seabird
Článek Ozone effects on blood biomarkers of systemic inflammation, oxidative stress, endothelial function, and thrombosis: The Multicenter Ozone Study in oldEr Subjects (MOSES)
Článek Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis
Článek Prevalence of Epstein-Barr virus, human papillomavirus, cytomegalovirus and herpes simplex virus type 1 in patients with diabetes mellitus type 2 in south-eastern Poland
Článek Social and structural factors associated with substance use within the support network of adults living in precarious housing in a socially marginalized neighborhood of Vancouver, Canada
Článek Characterization of Monkeypox virus dissemination in the black-tailed prairie dog (Cynomys ludovicianus) through in vivo bioluminescent imaging
Článek LPS induces inflammatory chemokines via TLR-4 signalling and enhances the Warburg Effect in THP-1 cells
Článek How do seabirds modify their search behaviour when encountering fishing boats?
Článek The diagnostic value of pleural fluid homocysteine in malignant pleural effusion
Článek Spatio-temporal variations in wheat aphid populations and their natural enemies in four agro-ecological zones of Pakistan
Článek Factors associated with burnout amongst healthcare workers providing HIV care in Malawi
Článek A high-density exome capture genotype-by-sequencing panel for forestry breeding in Pinus radiata
Článek Self-management action and motivation of Pacific adults in New Zealand with end-stage renal disease
Článek Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)
Článek A prospective randomized trial on abacavir/lamivudine plus darunavir/ritonavir or raltegravir in HIV-positive drug-naïve patients with CD4<200 cells/uL (the PRADAR study)
Článek Analyzing data from the digital healthcare exchange platform for surveillance of antibiotic prescriptions in primary care in urban Kenya: A mixed-methods study
Článek Multi-dimensional evaluation of response to salt stress in wheat
Článek Tuberculosis in the wild boar: Frequentist and Bayesian estimations of diagnostic test parameters when Mycobacterium bovis is present in wild boars but at low prevalence
Článek Enhanced mechanical and thermal properties of electrically conductive TPNR/GNP nanocomposites assisted with ultrasonication
Článek Geospatial modeling of microcephaly and zika virus spread patterns in Brazil
Článek Maternal cardiovascular-related single nucleotide polymorphisms, genes, and pathways associated with early-onset preeclampsia
Článek A ten-year review of ESBL and non-ESBL Escherichia coli bloodstream infections among children at a tertiary referral hospital in South Africa
Článek Reference values for the cervical spinal canal and the vertebral bodies by MRI in a general population
Článek Dynamic mechanical characteristics and failure mode of serpentine under a three-dimensional high static load and frequent dynamic disturbance
Článek Incorporating statistical strategy into image analysis to estimate effects of steam and allyl isocyanate on weed control
Článek Regulation of NF-κB- and STAT1-mediated plasmacytoid dendritic cell functions by A20
Článek The erythrocyte membrane stability is associated with sleep time and social jetlag in shift workers
Článek New space-time block codes from spectral norm
Článek Mexican BRCA1 founder mutation: Shortening the gap in genetic assessment for hereditary breast and ovarian cancer patients
Článek GIVE me your attention: Differentiating goal identification and goal execution components of the anti-saccade effect
Článek Maternal serum retinol, 25(OH)D and 1,25(OH)2D concentrations during pregnancy and peak bone mass and trabecular bone score in adult offspring at 26-year follow-up
Článek Self Multi-Head Attention-based Convolutional Neural Networks for fake news detection
Článek Why are Chinese workers so unhappy? A comparative cross-national analysis of job satisfaction, job expectations, and job attributes
Článek Fecal indicator bacteria and virus removal in stormwater biofilters: Effects of biochar, media saturation, and field conditioning
Článek Personalized microstructural evaluation using a Mahalanobis-distance based outlier detection strategy on epilepsy patients’ DTI data – Theory, simulations and example cases
Článek Radiographic assessment of pectoral flipper bone maturation in bottlenose dolphins (Tursiops truncatus), as a novel technique to accurately estimate chronological age
Článek Intraoperative measurement of intraventricular pressure in dogs with communicating internal hydrocephalus
Článek Relative importance of gene effects for nitrogen-use efficiency in popcorn
Článek Comparative analysis uncovers the limitations of current molecular detection methods for Fusarium oxysporum f. sp. cubense race 4 strains
Článek The accuracy of the frontal extent in stereoscopic environments: A comparison of direct selection and virtual cursor techniques
Článek Confronting pastoralists’ knowledge of cattle breeds raised in the extensive production systems of Benin with multivariate analyses of morphological traits
Článek CH(II), a cerebroprotein hydrolysate, exhibits potential neuro-protective effect on Alzheimer’s disease
Článek Decontamination of aerosolised bacteria from a pig farm environment using a pH neutral electrochemically activated solution (Ecas4 anolyte)
Článek Transcriptomic changes triggered by ouabain in rat cerebellum granule cells: Role of α3- and α1-Na+,K+-ATPase-mediated signaling
Článek Simultaneous transcriptome analysis of oil palm clones and Phytophthora palmivora reveals oil palm defense strategies
Článek Antifungal effects of a 1,3,4-thiadiazole derivative determined by cytochemical and vibrational spectroscopic studies
Článek Risk factors for unsuccessful tuberculosis treatment outcomes in children
Článek CRISPR-based tools for targeted transcriptional and epigenetic regulation in plants
Článek Reliability of high-resolution ultrasound and magnetic resonance arthrography of the shoulder in patients with sports-related shoulder injuries
Článek Spatial-temporal variation characteristics and evolution of the global industrial robot trade: A complex network analysis
Článek Axial variation of deoxyhemoglobin density as a source of the low-frequency time lag structure in blood oxygenation level-dependent signals
Článek On the sustainability of a family planning program in Nigeria when funding ends
Článek The epidemiology of antidepressant use in South Korea: Does short-term antidepressant use affect the relapse and recurrence of depressive episodes?
Článek Injury severity level and associated factors among road traffic accident victims attending emergency department of Tirunesh Beijing Hospital, Addis Ababa, Ethiopia: A cross sectional hospital-based study
Článek How chimpanzees (Pan troglodytes) share the spoils with collaborators and bystanders
Článek Seed treatment using methyl jasmonate induces resistance to rice water weevil but reduces plant growth in rice
Článek Placebo analgesia induced by verbal suggestion in the context of experimentally induced fear and anxiety
Článek Reconsidering the associations between self-reported alcohol use disorder and mental health problems in the light of co-occurring addictions in young Swiss men
Článek Learning to assist smokers through encounters with standardized patients: An innovative training for physicians in an Eastern European country
Článek Serum E-selectin concentration is associated with risk of metabolic syndrome in females
Článek Lipid and fatty acid dynamics by maternal Pacific bluefin tuna
Článek Adherence to evidence-based recommendations for surgical site infection prevention: Results among Italian surgical ward nurses
Článek Novel synergistic fungicidal mixtures of oxathiapiprolin protect sunflower seeds from downy mildew caused by Plasmopara halstedii
Článek Adjustment of a numerical model for pore pressure generation during an earthquake
Článek The risk, perceived and actual, of developing type 2 diabetes mellitus for mothers of preschool children in urban China
Článek Effects of turning frequency on the nutrients of Camellia oleifera shell co-compost with goat dung and evaluation of co-compost maturity
Článek Imaging disease activity of rheumatoid arthritis by macrophage targeting using second generation translocator protein positron emission tomography tracers
Článek Distribution of SET/I2PP2A protein in gastrointestinal tissues
Článek Spontaneous lymphoblastoid cell lines from patients with Epstein-Barr virus infection show highly variable proliferation characteristics that correlate with the expression levels of viral microRNAs
Článek Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor α-motoneurons: Anatomical and neurochemical study
Článek Anatomy of a demand shock: Quantitative analysis of crowding in hospital emergency departments in Victoria, Australia during the 2009 influenza pandemic
Článek Oregano essential oil vapour prevents Plasmopara viticola infection in grapevine (Vitis Vinifera) and primes plant immunity mechanisms
Článek Dual inhibitory action of trazodone on dorsal raphe serotonergic neurons through 5-HT1A receptor partial agonism and α1-adrenoceptor antagonism
Článek Optimising poly(lactic-co-glycolic acid) microparticle fabrication using a Taguchi orthogonal array design-of-experiment approach
Článek Repetitive finger movement and circle drawing in persons with Parkinson’s disease
Článek The association between family members’ migration and cognitive function among people left behind in China
Článek Association between long-term adherence to class-I recommended medications and risk for potentially preventable heart failure hospitalizations among younger adults
Článek Differential role of r-met-hu G-CSF on male reproductive function and development in prepubertal domestic mammals
Článek Agreement between the spatiotemporal gait parameters from two different wearable devices and high-speed video analysis
Článek In-hospital cardiopulmonary resuscitation of patients with cirrhosis: A population-based analysis
Článek Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
Článek Someone like me: Size-assortative pairing and mating in an Amazonian fish, sailfin tetra Crenuchus spilurus
Článek Seasonality of antenatal care attendance, maternal dietary intake, and fetal growth in the VHEMBE birth cohort, South Africa
Článek Are trials of psychological and psychosocial interventions for schizophrenia and psychosis included in the NICE guidelines pragmatic? A systematic review
Článek Incidence of retinal vein occlusion with long-term exposure to ambient air pollution
Článek Inclusion of enclosed hydration effects in the binding free energy estimation of dopamine D3 receptor complexes
Článek Intensity modulated radiation therapy following lumpectomy in early-stage breast cancer: Patterns of use and cost consequences among Medicare beneficiaries
Článek The ‘Saw but Forgot’ error: A role for short-term memory failures in understanding junction crashes?
Článek Multiscale Modelling Tool: Mathematical modelling of collective behaviour without the maths
Článek A comparison of machine learning algorithms for the surveillance of autism spectrum disorder
Článek Drivers of deforestation in the basin of the Usumacinta River: Inference on process from pattern analysis using generalised additive models
Článek Structure of Dictyostelium discoideum telomeres. Analysis of possible replication mechanisms
Článek Multidrug-resistant profile and prevalence of extended spectrum β-lactamase and carbapenemase production in fermentative Gram-negative bacilli recovered from patients and specimens referred to National Reference Laboratory, Addis Ababa, Ethiopia
Článek Primary myelofibrosis marrow-derived CD14+/CD34- monocytes induce myelofibrosis-like phenotype in immunodeficient mice and give rise to megakaryocytes
Článek Intersubject MVPD: Empirical comparison of fMRI denoising methods for connectivity analysis
Článek Why Cohen’s Kappa should be avoided as performance measure in classification
Článek A novel strategy for interpreting the T-SPOT.TB test results read by an ELISPOT plate imager
Článek Computational identification of key genes that may regulate gene expression reprogramming in Alzheimer’s patients
Článek NPHP proteins are binding partners of nucleoporins at the base of the primary cilium
Článek Intensive measures of luminescence in GaN/InGaN heterostructures
Článek The effect of a web-based training for improving primary health care providers’ knowledge about diabetes mellitus management in rural China: A pre-post intervention study
Článek Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
Článek Quantitative magnetic resonance imaging indicates brain tissue alterations in patients after liver transplantation
Článek DNA analysis of Castanea sativa (sweet chestnut) in Britain and Ireland: Elucidating European origins and genepool diversity
Článek Forecasting the impact of population ageing on tuberculosis incidence
Článek Conformation and mechanical property of rpoS mRNA inhibitory stem studied by optical tweezers and X-ray scattering
Článek Voxelwise statistical methods to localize practice variation in brain tumor surgery
Článek Identification and evolutionary characterization of salt-responsive transcription factors in the succulent halophyte Suaeda fruticosa
Článek Integrating temperature-dependent life table data into Insect Life Cycle Model for predicting the potential distribution of Scapsipedus icipe Hugel & Tanga
Článek Rollout of ShangRing circumcision with active surveillance for adverse events and monitoring for uptake in Kenya
Článek Comparative phenotypic profiling of the JAK2 inhibitors ruxolitinib, fedratinib, momelotinib, and pacritinib reveals distinct mechanistic signatures
Článek Analyzing a networked social algorithm for collective selection of representative committees
Článek Gene expression profiling of skeletal myogenesis in human embryonic stem cells reveals a potential cascade of transcription factors regulating stages of myogenesis, including quiescent/activated satellite cell-like gene expression
Článek Ex vivo physiological compression of human osteoarthritis cartilage modulates cellular and matrix components
Článek EFForTS-LGraf: A landscape generator for creating smallholder-driven land-use mosaics
Článek Impact of the severity of negative energy balance on gene expression in the subcutaneous adipose tissue of periparturient primiparous Holstein dairy cows: Identification of potential novel metabolic signals for the reproductive system
Článek Precision and consistency of the passive leg raising maneuver for determining fluid responsiveness with bioreactance non-invasive cardiac output monitoring in critically ill patients and healthy volunteers
Článek Optimizing bacterial DNA extraction in urine
Článek Adherences to oral nutritional supplementation among hospital outpatients: An online cross-sectional survey in Japan
Článek Effects of inorganic nitrogen and litters of Masson Pine on soil organic carbon decomposition
Článek Antioxidant properties of potato tubers (Solanum tuberosum L.) as a consequence of genetic potential and growing conditions
Článek Transcatheter aortic valve implantation versus conservative management for severe aortic stenosis in real clinical practice
Článek “I put it in my head that the supplement would help me”: Open-placebo improves exercise performance in female cyclists
Článek Automated diagnosis of heart valve degradation using novelty detection algorithms and machine learning
Článek Spatiotemporal clustering of malaria in southern-central Ethiopia: A community-based cohort study
Článek Studying the link between physiological performance of Crotalaria ochroleuca and the distribution of Ca, P, K and S in seeds with X-ray fluorescence
Článek Characterization of bony changes localized to the cervical articular processes in a mixed population of horses
Článek Heat shock-induced chaperoning by Hsp70 is enabled in-cell
Článek Disability and its association with sociodemographic factors among elderly persons residing in an urban resettlement colony, New Delhi, India
Článek Correlates of prenatal and postnatal mother-to-infant bonding quality: A systematic review
Článek IGFBP2 promotes immunosuppression associated with its mesenchymal induction and FcγRIIB phosphorylation in glioblastoma
Článek Dietary supplementation with the extract from Eucommia ulmoides leaves changed epithelial restitution and gut microbial community and composition of weanling piglets
Článek How did that interaction make you feel? The relationship between quality of everyday social experiences and emotion in people with and without schizophrenia
Článek Effects of Lyse-It on endonuclease fragmentation, function and activity
Článek Overexpression of rice gene OsATG8b confers tolerance to nitrogen starvation and increases yield and nitrogen use efficiency (NUE) in Arabidopsis
Článek Gait can reveal sleep quality with machine learning models
Článek Blast vacuolization in AML patients indicates adverse-risk AML and is associated with impaired survival after intensive induction chemotherapy
Článek Mitochondrial fragmentation and network architecture in degenerative diseases
Článek Quadriceps muscle strength is a discriminant predictor of dependence in daily activities in nursing home residents
Článek Discovery of novel West Nile Virus protease inhibitor based on isobenzonafuranone and triazolic derivatives of eugenol and indan-1,3-dione scaffolds
Článek Skin-associated lactic acid bacteria from North American bullfrogs as potential control agents of Batrachochytrium dendrobatidis
Článek Microbiota of MR1 deficient mice confer resistance against Clostridium difficile infection
Článek The responses of extracellular enzyme activities and microbial community composition under nitrogen addition in an upland soil
Článek Post-translational S-glutathionylation of cofilin increases actin cycling during cocaine seeking
Článek Absolute pitch can be learned by some adults
Článek Anger while driving in Mexico City
Článek Glomerular hyperfiltration may be a novel risk factor of restrictive spirometry pattern: Analysis of the Korea National Health and Nutrition Examination Survey (KNHANES) 2009-2015
Článek Survival of infants ≤24 months of age with brain tumors: A population-based study using the SEER database
Článek Multiple evoked and induced alpha modulations in a visual attention task: Latency, amplitude and topographical profiles
Článek Heart rate variability reduction is related to a high amount of visceral adiposity in healthy young women
Článek Serum and urinary metabolomics and outcomes in cirrhosis
Článek Optimal threshold of adherence to lipid lowering drugs in predicting acute coronary syndrome, stroke, or mortality: A cohort study
Článek Systemic antibiotic prophylaxis does not affect infectious complications in pediatric burn injury: A meta-analysis
Článek How Knowledge Stock Exchanges can increase student success in Massive Open Online Courses
Článek CT-perfusion in peripheral arterial disease – Correlation with angiographic and hemodynamic parameters
Článek Pattern of health care utilization and traditional and complementary medicine use among Ebola survivors in Sierra Leone
Článek Respiratory variations in pulse pressure and photoplethysmographic waveform amplitude during positive expiratory pressure and continuous positive airway pressure in a model of progressive hypovolemia
Článek Dorsal tongue porphyrin autofluorescence and Candida saprophytism: A prospective observational study
Článek A usability study to improve a clinical decision support system for the prescription of antibiotic drugs
Článek Occurrence of and risk factors for extended-spectrum cephalosporin-resistant Enterobacteriaceae determined by sampling of all Norwegian broiler flocks during a six month period
Článek Healthcare facility-based strategies to improve tuberculosis testing and linkage to care in non-U.S.-born population in the United States: A systematic review
Článek Correction: Reward abundance interferes with error-based learning in a visuomotor adaptation task
Článek Correction: Potential user interest in new long-acting contraceptives: Results from a mixed methods study in Burkina Faso and Uganda
Článek Correction: Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits
Článek Critically ill patients with community-onset intraabdominal infections: Influence of healthcare exposure on resistance rates and mortality
Článek Validating a popular outpatient antibiotic database to reliably identify high prescribing physicians for patients 65 years of age and older
Článek Metabolisable energy content in canine and feline foods is best predicted by the NRC2006 equation
Článek Group conquers efficacy: Preschoolers’ imitation under conflict between minimal group membership and behavior efficacy
Článek Warmth and competence stereotypes about immigrant groups in Germany
Článek Social resistance drives persistent transmission of Ebola virus disease in Eastern Democratic Republic of Congo: A mixed-methods study
Článek ER stress activation in the intestinal mucosa but not in mesenteric adipose tissue is associated with inflammation in Crohn’s disease patients
Článek Enhancing percutaneous pedicle screw fixation with hydroxyapatite granules: A biomechanical study using an osteoporotic bone model
Článek Optimum plant density for crowding stress tolerant processing sweet corn
Článek Sign and goal tracker rats process differently the incentive salience of a conditioned stimulus
Článek Interocular symmetry, intraobserver repeatability, and interobserver reliability of cone density measurements in the 13-lined ground squirrel
Článek Plasma metabolite biomarkers for multiple system atrophy and progressive supranuclear palsy
Článek Knowledge, attitudes and practices of livestock and aquaculture producers regarding antimicrobial use and resistance in Vietnam
Článek Scientific sinkhole: The pernicious price of formatting
Článek Resource consumption of multi-substance users in the emergency room: A neglected patient group
Článek Association between distress and knowledge among parents of autistic children
Článek Surgical approach for complete cochlear coverage in EAS-patients after residual hearing loss
Článek The impact of a rapid molecular identification test on positive blood cultures from critically ill with bacteremia: A pre-post intervention study
Článek Shortening duration of ertapenem in outpatient parenteral antimicrobial therapy for complicated urinary tract infections: A retrospective study
Článek Measurement and analysis of partial lightning currents in a head phantom
Článek Within species expressed genetic variability and gene expression response to different temperatures in the rotifer Brachionus calyciflorus sensu stricto
Článek Repeated transspinal stimulation decreases soleus H-reflex excitability and restores spinal inhibition in human spinal cord injury
Článek Expression of Concern: Resveratrol Enhances Antitumor Activity of TRAIL in Prostate Cancer Xenografts through Activation of FOXO Transcription Factor
Článek Correlation of CT texture changes with treatment response during radiation therapy for esophageal cancer: An exploratory study
Článek Modeling spatial variation in density of golden eagle nest sites in the western United States
Článek Retraction: Autophagy in Muscle of Glucose-Infusion Hyperglycemia Rats and Streptozotocin-Induced Hyperglycemia Rats via Selective Activation of m-TOR or FoxO3
Článek Correction: In Porphyromonas gingivalis VimF Is Involved in Gingipain Maturation through the Transfer of Galactose
Článek Gibberellic acid in Citrus spp. flowering and fruiting: A systematic review
Článek Discharge care quality in hospitalised elderly patients: Extended validation of the Discharge Care Experiences Survey
Článek A novel therapeutic strategy for esophageal varices using endoscopic treatment combined with splenic artery embolization according to the Child-Pugh classification
Článek Correction: Development of a high-throughput assay to measure measles neutralizing antibodies
Článek Correction: Provider preference for payment method under a national health insurance scheme: A survey of health insurance-credentialed health care providers in Ghana
Článek Correction: Second-line HIV treatment failure in sub-Saharan Africa: A systematic review and meta-analysis
Článek Potential impact of efflux pump genes in mediating rifampicin resistance in clinical isolates of Mycobacterium tuberculosis from India
Článek Feed-forward visual processing suffices for coarse localization but fine-grained localization in an attention-demanding context needs feedback processing
Článek Level Set method-based two-dimensional numerical model for simulation of nonuniform open-channel flow
Článek Compared to non-drinkers, individuals who drink alcohol have a more favorable multisystem physiologic risk score as measured by allostatic load
Článek Effect of poor glycaemic control on plasma levels and activity of protein C, protein S, and antithrombin III in type 2 diabetes mellitus
Článek Retraction: IL-21 Regulates the Differentiation of a Human γδ T Cell Subset Equipped with B Cell Helper Activity
Článek Sugar transporters in Fabaceae, featuring SUT MST and SWEET families of the model plant Medicago truncatula and the agricultural crop Pisum sativum
Článek Prediction of lymphovascular space invasion in endometrial cancer using the 55-gene signature selected by DNA microarray analysis
Článek Research on multi-agent genetic algorithm based on tabu search for the job shop scheduling problem
Článek Diagnosing the current state of out-of-field teaching in high school science and mathematics
Článek Sensitivity of the fasciae to sex hormone levels: Modulation of collagen-I, collagen-III and fibrillin production
Článek Comorbidity of age-related macular degeneration with Alzheimer’s disease: A histopathologic case-control study
Článek Acute ex vivo changes in brain white matter diffusion tensor metrics
Článek Regulatory interaction between the ZPBP2-ORMDL3/Zpbp2-Ormdl3 region and the circadian clock
Článek The effect of bacteria on planula-larvae settlement and metamorphosis in the octocoral Rhytisma fulvum fulvum
Článek Sodium alginate potentiates antioxidant defense and PR proteins against early blight disease caused by Alternaria solani in Solanum lycopersicum Linn.
Článek Ground reaction forces and muscle activity while walking on sand versus stable ground in individuals with pronated feet compared with healthy controls
Článek Correction: Roadway traffic crash prediction using a state-space model based support vector regression approach
Článek MET as resistance factor for afatinib therapy and motility driver in gastric cancer cells
Článek Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice
Článek Clinical features of pulmonary embolism in patients with lung cancer: A meta-analysis
Článek Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota
Článek Factors restraining the population growth of Varroa destructor in Ethiopian honey bees (Apis mellifera simensis)
Článek Coastal proximity of populations in 22 Pacific Island Countries and Territories
Článek DKK1 promotes hepatocellular carcinoma inflammation, migration and invasion: Implication of TGF-β1
Článek Genetic characterization of Angiostrongylus larvae and their intermediate host, Achatina fulica, in Thailand
Článek Targeted in-vitro-stimulation reveals highly proliferative multi-virus-specific human central memory T cells as candidates for prophylactic T cell therapy
Článek Influence of ocean circulation and the Kuroshio large meander on the 2018 Japanese eel recruitment season
Článek Growth response of the ichthyotoxic haptophyte, Prymnesium parvum Carter, to changes in sulfate and fluoride concentrations
Článek Letrozole treatment of pubertal female mice results in activational effects on reproduction, metabolism and the gut microbiome
Článek Prognostic nomogram predicts overall survival in pulmonary large cell neuroendocrine carcinoma
Článek Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)
Článek Characteristics of proximal early gastric cancer differentiating distal early gastric cancer
Článek Retraction: Impacts of rock properties on Danxia landform formation based on lithological experiments at Kongtongshan National Geopark, northwest China
Článek Correction: SVR12 rates higher than 99% after sofosbuvir/velpatasvir combination in HCV infected patients with F0-F1 fibrosis stage: A real world experience
Článek Retraction: Plexin-B1 Activates NF-κB and IL-8 to Promote a Pro-Angiogenic Response in Endothelial Cells
Článek Relationship between cognitive behavioral variables and mental health status among university students: A meta-analysis
Článek Association between temperature, sunlight hours and alcohol consumption
Článek Using graph learning to understand adverse pregnancy outcomes and stress pathways
Článek Correction: The step-to-step transition mode: A potential indicator of first-fall risk in elderly adults?
Článek Surgical referral systems in low- and middle-income countries: A review of the evidence
Článek In vivo clearance of nanoparticles by transcytosis across alveolar epithelial cells
Článek Correction: The Economic Impact of Malignant Catarrhal Fever on Pastoralist Livelihoods
Článek Correction: Identification of miRNAs involved in fruit ripening by deep sequencing of Olea europaea L. transcriptome
Článek Correction: Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach
Článek Correction: Vascular Endothelial Growth Factor Receptor-2 Couples Cyclo-Oxygenase-2 with Pro-Angiogenic Actions of Leptin on Human Endothelial Cells
Článek Correction: Plant traits linked to field-scale flammability metrics in prescribed burns in Eucalyptus forest
Článek Correction: Maturation of three-dimensional, hiPSC-derived cardiomyocyte spheroids utilizing cyclic, uniaxial stretch and electrical stimulation
Článek Correction: To keep or not to keep? Decision making in adolescent pregnancies in Jamestown, Ghana
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PLOS One

2019 Číslo 9
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Aktuální možnosti diagnostiky a léčby litiáz
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Autori: MUDr. Tomáš Ürge, PhD.
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